Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo

AIM: To study <i>in vivo</i> the effect of androgen on mice tear film stability and Mucins expressions in corneal epithelial cells in BALB/c mice after orchectomy.<p>METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by...

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Main Authors: Li Li, Xuan Zheng, Shuang-Mei Wang, Ge Gao, Qian-Yan Kang
Format: Article
Language:English
Published: Press of International Journal of Ophthalmology (IJO PRESS) 2016-07-01
Series:Guoji Yanke Zazhi
Subjects:
Online Access:http://ies.ijo.cn/cn_publish/2016/7/201607006.pdf
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author Li Li
Xuan Zheng
Shuang-Mei Wang
Ge Gao
Qian-Yan Kang
author_facet Li Li
Xuan Zheng
Shuang-Mei Wang
Ge Gao
Qian-Yan Kang
author_sort Li Li
collection DOAJ
description AIM: To study <i>in vivo</i> the effect of androgen on mice tear film stability and Mucins expressions in corneal epithelial cells in BALB/c mice after orchectomy.<p>METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by radioimmunoassay. Break-up time(BUT)of tear film was tested in the different experimental points. Mice corneal epithelia were peeled and MUC1 and MUC4 mRNA and protein levels were measured by RT-PCR and Western blot. <p>RESULTS: The serum androgen concentration reduced to 0ng/μL at 1wk after orchiectomy. The BUT values were 68.33±12.86s, 62.47±3.75s, 58.67±10.03s, 47.17±7.64s, 39.51±3.39s, 32.67±3.88s and 31.00±2.36s in the normal control group, sham group and in orchectomy group at 1, 2, 4, 6 and 8wk, respectively, and the BUTs were significantly shorter in the orchectomy at 2, 4, 6 and 8wk groups than those in the normal control group(all at <i>P</i><0.05). MUC1 and MUC4 mRNA and proteins levels decreased with androgen level lowering(<i>P</i><0.05). Mucin1 level was the lowest at 2wk after orchiectomy, and the lowest Mucin4 level was found at 1wk after orchiectomy. <p>CONCLUSION:<i>In vivo</i>, androgen regulates Mucins expressions in mice corneal epithelial cells, makes BUT shorter,and influence the stability of tear film.
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spelling doaj.art-2db5bfbb788a484fbeaab606fd6bb9c62022-12-21T22:46:12ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232016-07-011671228123110.3980/j.issn.1672-5123.2016.7.06Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivoLi Li0Xuan Zheng1Shuang-Mei Wang2Ge Gao3Qian-Yan Kang4Department of Ophthalmology, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an 710061, Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an No.1 Hospital, Xi'an 710002, Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an No.4 Hospital, Xi'an 710004,Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an No.4 Hospital, Xi'an 710004,Shaanxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an 710061, Shaanxi Province, ChinaAIM: To study <i>in vivo</i> the effect of androgen on mice tear film stability and Mucins expressions in corneal epithelial cells in BALB/c mice after orchectomy.<p>METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by radioimmunoassay. Break-up time(BUT)of tear film was tested in the different experimental points. Mice corneal epithelia were peeled and MUC1 and MUC4 mRNA and protein levels were measured by RT-PCR and Western blot. <p>RESULTS: The serum androgen concentration reduced to 0ng/μL at 1wk after orchiectomy. The BUT values were 68.33±12.86s, 62.47±3.75s, 58.67±10.03s, 47.17±7.64s, 39.51±3.39s, 32.67±3.88s and 31.00±2.36s in the normal control group, sham group and in orchectomy group at 1, 2, 4, 6 and 8wk, respectively, and the BUTs were significantly shorter in the orchectomy at 2, 4, 6 and 8wk groups than those in the normal control group(all at <i>P</i><0.05). MUC1 and MUC4 mRNA and proteins levels decreased with androgen level lowering(<i>P</i><0.05). Mucin1 level was the lowest at 2wk after orchiectomy, and the lowest Mucin4 level was found at 1wk after orchiectomy. <p>CONCLUSION:<i>In vivo</i>, androgen regulates Mucins expressions in mice corneal epithelial cells, makes BUT shorter,and influence the stability of tear film.http://ies.ijo.cn/cn_publish/2016/7/201607006.pdfandrogentear film break timemucins: corneal epithelium cellmucins: corneal epithelium cell
spellingShingle Li Li
Xuan Zheng
Shuang-Mei Wang
Ge Gao
Qian-Yan Kang
Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo
Guoji Yanke Zazhi
androgen
tear film break time
mucins: corneal epithelium cell
mucins: corneal epithelium cell
title Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo
title_full Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo
title_fullStr Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo
title_full_unstemmed Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo
title_short Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo
title_sort effect of androgen on mice tear film stability and mucins expressions in mice corneal epithelial cells i in vivo
topic androgen
tear film break time
mucins: corneal epithelium cell
mucins: corneal epithelium cell
url http://ies.ijo.cn/cn_publish/2016/7/201607006.pdf
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