Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo
AIM: To study <i>in vivo</i> the effect of androgen on mice tear film stability and Mucins expressions in corneal epithelial cells in BALB/c mice after orchectomy.<p>METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by...
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Press of International Journal of Ophthalmology (IJO PRESS)
2016-07-01
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Series: | Guoji Yanke Zazhi |
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Online Access: | http://ies.ijo.cn/cn_publish/2016/7/201607006.pdf |
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author | Li Li Xuan Zheng Shuang-Mei Wang Ge Gao Qian-Yan Kang |
author_facet | Li Li Xuan Zheng Shuang-Mei Wang Ge Gao Qian-Yan Kang |
author_sort | Li Li |
collection | DOAJ |
description | AIM: To study <i>in vivo</i> the effect of androgen on mice tear film stability and Mucins expressions in corneal epithelial cells in BALB/c mice after orchectomy.<p>METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by radioimmunoassay. Break-up time(BUT)of tear film was tested in the different experimental points. Mice corneal epithelia were peeled and MUC1 and MUC4 mRNA and protein levels were measured by RT-PCR and Western blot. <p>RESULTS: The serum androgen concentration reduced to 0ng/μL at 1wk after orchiectomy. The BUT values were 68.33±12.86s, 62.47±3.75s, 58.67±10.03s, 47.17±7.64s, 39.51±3.39s, 32.67±3.88s and 31.00±2.36s in the normal control group, sham group and in orchectomy group at 1, 2, 4, 6 and 8wk, respectively, and the BUTs were significantly shorter in the orchectomy at 2, 4, 6 and 8wk groups than those in the normal control group(all at <i>P</i><0.05). MUC1 and MUC4 mRNA and proteins levels decreased with androgen level lowering(<i>P</i><0.05). Mucin1 level was the lowest at 2wk after orchiectomy, and the lowest Mucin4 level was found at 1wk after orchiectomy. <p>CONCLUSION:<i>In vivo</i>, androgen regulates Mucins expressions in mice corneal epithelial cells, makes BUT shorter,and influence the stability of tear film. |
first_indexed | 2024-12-14T21:52:15Z |
format | Article |
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institution | Directory Open Access Journal |
issn | 1672-5123 1672-5123 |
language | English |
last_indexed | 2024-12-14T21:52:15Z |
publishDate | 2016-07-01 |
publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
record_format | Article |
series | Guoji Yanke Zazhi |
spelling | doaj.art-2db5bfbb788a484fbeaab606fd6bb9c62022-12-21T22:46:12ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232016-07-011671228123110.3980/j.issn.1672-5123.2016.7.06Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivoLi Li0Xuan Zheng1Shuang-Mei Wang2Ge Gao3Qian-Yan Kang4Department of Ophthalmology, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an 710061, Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an No.1 Hospital, Xi'an 710002, Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an No.4 Hospital, Xi'an 710004,Shaanxi Province, ChinaDepartment of Ophthalmology, Xi'an No.4 Hospital, Xi'an 710004,Shaanxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an 710061, Shaanxi Province, ChinaAIM: To study <i>in vivo</i> the effect of androgen on mice tear film stability and Mucins expressions in corneal epithelial cells in BALB/c mice after orchectomy.<p>METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by radioimmunoassay. Break-up time(BUT)of tear film was tested in the different experimental points. Mice corneal epithelia were peeled and MUC1 and MUC4 mRNA and protein levels were measured by RT-PCR and Western blot. <p>RESULTS: The serum androgen concentration reduced to 0ng/μL at 1wk after orchiectomy. The BUT values were 68.33±12.86s, 62.47±3.75s, 58.67±10.03s, 47.17±7.64s, 39.51±3.39s, 32.67±3.88s and 31.00±2.36s in the normal control group, sham group and in orchectomy group at 1, 2, 4, 6 and 8wk, respectively, and the BUTs were significantly shorter in the orchectomy at 2, 4, 6 and 8wk groups than those in the normal control group(all at <i>P</i><0.05). MUC1 and MUC4 mRNA and proteins levels decreased with androgen level lowering(<i>P</i><0.05). Mucin1 level was the lowest at 2wk after orchiectomy, and the lowest Mucin4 level was found at 1wk after orchiectomy. <p>CONCLUSION:<i>In vivo</i>, androgen regulates Mucins expressions in mice corneal epithelial cells, makes BUT shorter,and influence the stability of tear film.http://ies.ijo.cn/cn_publish/2016/7/201607006.pdfandrogentear film break timemucins: corneal epithelium cellmucins: corneal epithelium cell |
spellingShingle | Li Li Xuan Zheng Shuang-Mei Wang Ge Gao Qian-Yan Kang Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo Guoji Yanke Zazhi androgen tear film break time mucins: corneal epithelium cell mucins: corneal epithelium cell |
title | Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo |
title_full | Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo |
title_fullStr | Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo |
title_full_unstemmed | Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo |
title_short | Effect of androgen on mice tear film stability and Mucins expressions in mice corneal epithelial cells <i>in vivo |
title_sort | effect of androgen on mice tear film stability and mucins expressions in mice corneal epithelial cells i in vivo |
topic | androgen tear film break time mucins: corneal epithelium cell mucins: corneal epithelium cell |
url | http://ies.ijo.cn/cn_publish/2016/7/201607006.pdf |
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