Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities
The aims of this study were to characterize the curcumin/cyclodextrin polymer inclusion complex using X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and UV–vis spectroscopy, and to determine the antioxidant activity of this comple...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-05-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/23/5/1179 |
| _version_ | 1818858226875105280 |
|---|---|
| author | Jianping Chen Xiaoming Qin Saiyi Zhong Suhua Chen Weiming Su Ying Liu |
| author_facet | Jianping Chen Xiaoming Qin Saiyi Zhong Suhua Chen Weiming Su Ying Liu |
| author_sort | Jianping Chen |
| collection | DOAJ |
| description | The aims of this study were to characterize the curcumin/cyclodextrin polymer inclusion complex using X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and UV–vis spectroscopy, and to determine the antioxidant activity of this complex by methods of scavenging 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals assays and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals assays. The inhibitory effect of inclusion complex on A375 cells was also investigated by CCK-8 assay, Annexin-V/PI staining assay, and caspase activity assay. The results showed that the complex exhibited different physicochemical characteristics from that of free curcumin. Moreover, the inclusion complex exhibited novel antioxidant activity by scavenging the ABTS and DPPH free radicals and displayed higher antiproliferative activity on A375 cells. Further investigation revealed that inclusion complex could induce A375 cell apoptosis. These findings suggest that inclusion complex could be developed as a novel natural antioxidant with potential applications in cancer chemoprevention. |
| first_indexed | 2024-12-19T08:52:56Z |
| format | Article |
| id | doaj.art-2dbdc7ea971546f380d8353bbb3a94c7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T08:52:56Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2dbdc7ea971546f380d8353bbb3a94c72022-12-21T20:28:41ZengMDPI AGMolecules1420-30492018-05-01235117910.3390/molecules23051179molecules23051179Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative ActivitiesJianping Chen0Xiaoming Qin1Saiyi Zhong2Suhua Chen3Weiming Su4Ying Liu5College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, ChinaFaculty of Agricultural Science, Guangdong Ocean University, Zhanjiang 524088, ChinaThe aims of this study were to characterize the curcumin/cyclodextrin polymer inclusion complex using X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and UV–vis spectroscopy, and to determine the antioxidant activity of this complex by methods of scavenging 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals assays and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals assays. The inhibitory effect of inclusion complex on A375 cells was also investigated by CCK-8 assay, Annexin-V/PI staining assay, and caspase activity assay. The results showed that the complex exhibited different physicochemical characteristics from that of free curcumin. Moreover, the inclusion complex exhibited novel antioxidant activity by scavenging the ABTS and DPPH free radicals and displayed higher antiproliferative activity on A375 cells. Further investigation revealed that inclusion complex could induce A375 cell apoptosis. These findings suggest that inclusion complex could be developed as a novel natural antioxidant with potential applications in cancer chemoprevention.http://www.mdpi.com/1420-3049/23/5/1179curcuminβ-cyclodextrin polymerinclusion complexantioxidant activityanticancer activity |
| spellingShingle | Jianping Chen Xiaoming Qin Saiyi Zhong Suhua Chen Weiming Su Ying Liu Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities Molecules curcumin β-cyclodextrin polymer inclusion complex antioxidant activity anticancer activity |
| title | Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities |
| title_full | Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities |
| title_fullStr | Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities |
| title_full_unstemmed | Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities |
| title_short | Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities |
| title_sort | characterization of curcumin cyclodextrin polymer inclusion complex and investigation on its antioxidant and antiproliferative activities |
| topic | curcumin β-cyclodextrin polymer inclusion complex antioxidant activity anticancer activity |
| url | http://www.mdpi.com/1420-3049/23/5/1179 |
| work_keys_str_mv | AT jianpingchen characterizationofcurcumincyclodextrinpolymerinclusioncomplexandinvestigationonitsantioxidantandantiproliferativeactivities AT xiaomingqin characterizationofcurcumincyclodextrinpolymerinclusioncomplexandinvestigationonitsantioxidantandantiproliferativeactivities AT saiyizhong characterizationofcurcumincyclodextrinpolymerinclusioncomplexandinvestigationonitsantioxidantandantiproliferativeactivities AT suhuachen characterizationofcurcumincyclodextrinpolymerinclusioncomplexandinvestigationonitsantioxidantandantiproliferativeactivities AT weimingsu characterizationofcurcumincyclodextrinpolymerinclusioncomplexandinvestigationonitsantioxidantandantiproliferativeactivities AT yingliu characterizationofcurcumincyclodextrinpolymerinclusioncomplexandinvestigationonitsantioxidantandantiproliferativeactivities |