Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i>
Although the war between wheat and <i>Fusarium</i> has been widely investigated for years, long noncoding RNAs (lncRNAs), which have been proven to regulate important processes in the development and stress responses of plants, are still poorly known in wheat against <i>Fusarium.&l...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/11/10/1135 |
| _version_ | 1797552404241055744 |
|---|---|
| author | Xiaoxin Duan Xiushi Song Jianxin Wang Mingguo Zhou |
| author_facet | Xiaoxin Duan Xiushi Song Jianxin Wang Mingguo Zhou |
| author_sort | Xiaoxin Duan |
| collection | DOAJ |
| description | Although the war between wheat and <i>Fusarium</i> has been widely investigated for years, long noncoding RNAs (lncRNAs), which have been proven to regulate important processes in the development and stress responses of plants, are still poorly known in wheat against <i>Fusarium.</i> Herein, we systematically reveal the roles of wheat lncRNAs in the process of <i>Fusarium graminearum</i> infection by high-throughput RNA sequencing. Well over 4130 of the total 4276 differentially expressed lncRNAs were already specifically expressed at 12 h postinoculation (hpi), but only 89 of these were specifically expressed at 24 hpi, indicating that the initial stage was the crucial stage for lncRNA-mediated gene regulation of wheat defense against <i>F. graminearum</i>. Target analysis showed the lncRNAs participated in various biological stress processes and had exclusive regulation models at different infection stages. Further H<sub>2</sub>O<sub>2</sub> accumulation and protein ubiquitination assays supported this idea. Moreover, two lncRNAs (XLOC_302848 and XLOC_321638) were identified as Fusarium seedling blight resistance candidates by lncRNA-target expression pattern validation, and two lncRNAs (XLOC_113815, XLOC_123624) were Fusarium head blight resistance potential regulators by cross-validating the RNAseq data with the refined meta-QTL of wheat FHB resistance. These findings extend our knowledge on wheat lncRNAs response to <i>F. graminearum</i> attack and provide new insights for the functional and molecular research of future interactions between wheat and <i>Fusarium</i>. |
| first_indexed | 2024-03-10T16:00:33Z |
| format | Article |
| id | doaj.art-2dc580e4380d45a09ee6704fbabdc219 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T16:00:33Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-2dc580e4380d45a09ee6704fbabdc2192023-11-20T15:19:06ZengMDPI AGGenes2073-44252020-09-011110113510.3390/genes11101135Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i>Xiaoxin Duan0Xiushi Song1Jianxin Wang2Mingguo Zhou3Laboratory of Plant Disease Control and Phytopharmacy, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Plant Disease Control and Phytopharmacy, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Plant Disease Control and Phytopharmacy, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Plant Disease Control and Phytopharmacy, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaAlthough the war between wheat and <i>Fusarium</i> has been widely investigated for years, long noncoding RNAs (lncRNAs), which have been proven to regulate important processes in the development and stress responses of plants, are still poorly known in wheat against <i>Fusarium.</i> Herein, we systematically reveal the roles of wheat lncRNAs in the process of <i>Fusarium graminearum</i> infection by high-throughput RNA sequencing. Well over 4130 of the total 4276 differentially expressed lncRNAs were already specifically expressed at 12 h postinoculation (hpi), but only 89 of these were specifically expressed at 24 hpi, indicating that the initial stage was the crucial stage for lncRNA-mediated gene regulation of wheat defense against <i>F. graminearum</i>. Target analysis showed the lncRNAs participated in various biological stress processes and had exclusive regulation models at different infection stages. Further H<sub>2</sub>O<sub>2</sub> accumulation and protein ubiquitination assays supported this idea. Moreover, two lncRNAs (XLOC_302848 and XLOC_321638) were identified as Fusarium seedling blight resistance candidates by lncRNA-target expression pattern validation, and two lncRNAs (XLOC_113815, XLOC_123624) were Fusarium head blight resistance potential regulators by cross-validating the RNAseq data with the refined meta-QTL of wheat FHB resistance. These findings extend our knowledge on wheat lncRNAs response to <i>F. graminearum</i> attack and provide new insights for the functional and molecular research of future interactions between wheat and <i>Fusarium</i>.https://www.mdpi.com/2073-4425/11/10/1135wheat<i>Fusarium graminearum</i>lncRNAidentificationmeta-QTL |
| spellingShingle | Xiaoxin Duan Xiushi Song Jianxin Wang Mingguo Zhou Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i> Genes wheat <i>Fusarium graminearum</i> lncRNA identification meta-QTL |
| title | Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i> |
| title_full | Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i> |
| title_fullStr | Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i> |
| title_full_unstemmed | Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i> |
| title_short | Genome-Wide Identification and Characterization of <i>Fusarium graminearum</i>-Responsive lncRNAs in <i>Triticum aestivum</i> |
| title_sort | genome wide identification and characterization of i fusarium graminearum i responsive lncrnas in i triticum aestivum i |
| topic | wheat <i>Fusarium graminearum</i> lncRNA identification meta-QTL |
| url | https://www.mdpi.com/2073-4425/11/10/1135 |
| work_keys_str_mv | AT xiaoxinduan genomewideidentificationandcharacterizationofifusariumgraminearumiresponsivelncrnasinitriticumaestivumi AT xiushisong genomewideidentificationandcharacterizationofifusariumgraminearumiresponsivelncrnasinitriticumaestivumi AT jianxinwang genomewideidentificationandcharacterizationofifusariumgraminearumiresponsivelncrnasinitriticumaestivumi AT mingguozhou genomewideidentificationandcharacterizationofifusariumgraminearumiresponsivelncrnasinitriticumaestivumi |