Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs
Objective The hypothesis was that apparent ileal digestibility (AID), basal endogenous losses, and standardized ileal digestibility (SID) of amino acids (AA) are not affected by adding acid to collection containers or bags used to collect ileal digesta from pigs. Methods Twenty-four growing barrows...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2021-10-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-20-0838.pdf |
| _version_ | 1819130841850183680 |
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| author | Su A Lee Laia Blavi Diego M. D. L. Navarro Hans H. Stein |
| author_facet | Su A Lee Laia Blavi Diego M. D. L. Navarro Hans H. Stein |
| author_sort | Su A Lee |
| collection | DOAJ |
| description | Objective The hypothesis was that apparent ileal digestibility (AID), basal endogenous losses, and standardized ileal digestibility (SID) of amino acids (AA) are not affected by adding acid to collection containers or bags used to collect ileal digesta from pigs. Methods Twenty-four growing barrows (initial body weight: 77.8±4.5 kg) that were fitted with a T-cannula in the distal ileum were fed diets for three 7-d periods. An N-free diet and 3 diets containing corn, soybean meal, or wheat middlings as the sole source of AA were used. Within each period, each of the 4 diets were fed to 6 pigs. Among the 6 pigs, digesta from 3 pigs were collected in bags containing no HCl, whereas 40 mL of 3 N HCl was included in the bags used to collect digesta from the remaining 3 pigs. Every other bag collected from each pig was emptied into a container without adding HCl, whereas the remaining bags were added to a container along with 40 mL of 3 N HCl for each bag. All digesta were stored at −20°C immediately after collection. Data were analyzed using a model that included feed ingredient, HCl in bags, HCl in containers, and all 2-way and 3-way interactions as fixed effects. No 3-way interactions were significant, and data were, therefore, reanalyzed independently for each diet as a 2×2 factorial. Results There were no interactions between adding HCl to collection bags and to containers, and no effects of adding HCl to collection bags or containers for AID, basal endogenous losses, or SID of most AA were observed. Conclusion It is not necessary to add acid to digesta collection bags or collection containers if ileal digesta are stored at −20°C immediately after collection. |
| first_indexed | 2024-12-22T09:06:02Z |
| format | Article |
| id | doaj.art-2dc8bd7f2c65437db0c9b1bb0b5b4d65 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-12-22T09:06:02Z |
| publishDate | 2021-10-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-2dc8bd7f2c65437db0c9b1bb0b5b4d652022-12-21T18:31:35ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352021-10-0134101632164210.5713/ab.20.083824683Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigsSu A Lee0Laia Blavi1Diego M. D. L. Navarro2Hans H. Stein3 Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USAObjective The hypothesis was that apparent ileal digestibility (AID), basal endogenous losses, and standardized ileal digestibility (SID) of amino acids (AA) are not affected by adding acid to collection containers or bags used to collect ileal digesta from pigs. Methods Twenty-four growing barrows (initial body weight: 77.8±4.5 kg) that were fitted with a T-cannula in the distal ileum were fed diets for three 7-d periods. An N-free diet and 3 diets containing corn, soybean meal, or wheat middlings as the sole source of AA were used. Within each period, each of the 4 diets were fed to 6 pigs. Among the 6 pigs, digesta from 3 pigs were collected in bags containing no HCl, whereas 40 mL of 3 N HCl was included in the bags used to collect digesta from the remaining 3 pigs. Every other bag collected from each pig was emptied into a container without adding HCl, whereas the remaining bags were added to a container along with 40 mL of 3 N HCl for each bag. All digesta were stored at −20°C immediately after collection. Data were analyzed using a model that included feed ingredient, HCl in bags, HCl in containers, and all 2-way and 3-way interactions as fixed effects. No 3-way interactions were significant, and data were, therefore, reanalyzed independently for each diet as a 2×2 factorial. Results There were no interactions between adding HCl to collection bags and to containers, and no effects of adding HCl to collection bags or containers for AID, basal endogenous losses, or SID of most AA were observed. Conclusion It is not necessary to add acid to digesta collection bags or collection containers if ileal digesta are stored at −20°C immediately after collection.http://www.animbiosci.org/upload/pdf/ab-20-0838.pdfamino acidscollectiondigestibilityhydrogen chlorideileal digestapigs |
| spellingShingle | Su A Lee Laia Blavi Diego M. D. L. Navarro Hans H. Stein Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs Animal Bioscience amino acids collection digestibility hydrogen chloride ileal digesta pigs |
| title | Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs |
| title_full | Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs |
| title_fullStr | Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs |
| title_full_unstemmed | Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs |
| title_short | Addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn, soybean meal, or wheat middlings fed to growing pigs |
| title_sort | addition of hydrochloric acid to collection bags or collection containers did not change basal endogenous losses or ileal digestibility of amino acid in corn soybean meal or wheat middlings fed to growing pigs |
| topic | amino acids collection digestibility hydrogen chloride ileal digesta pigs |
| url | http://www.animbiosci.org/upload/pdf/ab-20-0838.pdf |
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