The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening
Problems with the male reproductive system are of both medical and social significance. As a rule, spermatozoa and seminal plasma proteomes are investigated separately to assess sperm quality. The current study aimed to compare ejaculate proteomes with spermatozoa and seminal plasma protein profiles...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/49 |
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| author | Timur Shkrigunov Victor Zgoda Peter Klimenko Anna Kozlova Maria Klimenko Andrey Lisitsa Mark Kurtser Natalia Petushkova |
| author_facet | Timur Shkrigunov Victor Zgoda Peter Klimenko Anna Kozlova Maria Klimenko Andrey Lisitsa Mark Kurtser Natalia Petushkova |
| author_sort | Timur Shkrigunov |
| collection | DOAJ |
| description | Problems with the male reproductive system are of both medical and social significance. As a rule, spermatozoa and seminal plasma proteomes are investigated separately to assess sperm quality. The current study aimed to compare ejaculate proteomes with spermatozoa and seminal plasma protein profiles regarding the identification of proteins related to fertility scores. A total of 1779, 715, and 2163 proteins were identified in the ejaculate, seminal plasma, and spermatozoa, respectively. Among these datasets, 472 proteins were shared. GO enrichment analysis of the common proteins enabled us to distinguish biological processes such as single fertilization (GO:0007338), spermatid development (GO:0007286), and cell motility (GO:0048870). Among the abundant terms for GO cellular components, zona pellucida receptor complex, sperm fibrous sheath, and outer dense fiber were revealed. Overall, we identified 139 testis-specific proteins. For these proteins, PPI networks that are common in ejaculate, spermatozoa, and seminal plasma were related to the following GO biological processes: cilium movement (GO:0003341), microtubule-based movement (GO:0007018), and sperm motility (GO:0097722). For ejaculate and spermatozoa, they shared 15 common testis-specific proteins with spermatogenesis (GO:0007283) and male gamete generation (GO:0048232). Therefore, we speculated that ejaculate-based proteomics could yield new insights into the peculiar reproductive physiology and spermatozoa function of men and potentially serve as an explanation for male infertility screening. |
| first_indexed | 2024-03-08T09:57:35Z |
| format | Article |
| id | doaj.art-2dc906e4a20b4318bd0ce069da571654 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:57:35Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-2dc906e4a20b4318bd0ce069da5716542024-01-29T13:46:45ZengMDPI AGBiomedicines2227-90592023-12-011214910.3390/biomedicines12010049The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility ScreeningTimur Shkrigunov0Victor Zgoda1Peter Klimenko2Anna Kozlova3Maria Klimenko4Andrey Lisitsa5Mark Kurtser6Natalia Petushkova7Laboratory of Protein Biochemistry and Pathology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Systems Biology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaDepartment of Obstetrics and Gynecology, Pirogov Russian National Research Medical University, 117997 Moscow, RussiaCenter of Scientific and Practical Education, Institute of Biomedical Chemistry, 119121 Moscow, RussiaCenter for Family Planning and Reproduction, Moscow Department of Health, 117209 Moscow, RussiaLaboratory of Protein Biochemistry and Pathology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaDepartment of Obstetrics and Gynecology, Pirogov Russian National Research Medical University, 117997 Moscow, RussiaLaboratory of Protein Biochemistry and Pathology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaProblems with the male reproductive system are of both medical and social significance. As a rule, spermatozoa and seminal plasma proteomes are investigated separately to assess sperm quality. The current study aimed to compare ejaculate proteomes with spermatozoa and seminal plasma protein profiles regarding the identification of proteins related to fertility scores. A total of 1779, 715, and 2163 proteins were identified in the ejaculate, seminal plasma, and spermatozoa, respectively. Among these datasets, 472 proteins were shared. GO enrichment analysis of the common proteins enabled us to distinguish biological processes such as single fertilization (GO:0007338), spermatid development (GO:0007286), and cell motility (GO:0048870). Among the abundant terms for GO cellular components, zona pellucida receptor complex, sperm fibrous sheath, and outer dense fiber were revealed. Overall, we identified 139 testis-specific proteins. For these proteins, PPI networks that are common in ejaculate, spermatozoa, and seminal plasma were related to the following GO biological processes: cilium movement (GO:0003341), microtubule-based movement (GO:0007018), and sperm motility (GO:0097722). For ejaculate and spermatozoa, they shared 15 common testis-specific proteins with spermatogenesis (GO:0007283) and male gamete generation (GO:0048232). Therefore, we speculated that ejaculate-based proteomics could yield new insights into the peculiar reproductive physiology and spermatozoa function of men and potentially serve as an explanation for male infertility screening.https://www.mdpi.com/2227-9059/12/1/49ejaculatespermatozoashotgun proteomicstestis-specific proteinsmale infertility |
| spellingShingle | Timur Shkrigunov Victor Zgoda Peter Klimenko Anna Kozlova Maria Klimenko Andrey Lisitsa Mark Kurtser Natalia Petushkova The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening Biomedicines ejaculate spermatozoa shotgun proteomics testis-specific proteins male infertility |
| title | The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening |
| title_full | The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening |
| title_fullStr | The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening |
| title_full_unstemmed | The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening |
| title_short | The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening |
| title_sort | application of ejaculate based shotgun proteomics for male infertility screening |
| topic | ejaculate spermatozoa shotgun proteomics testis-specific proteins male infertility |
| url | https://www.mdpi.com/2227-9059/12/1/49 |
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