Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction
Cardiac dysfunction accelerates the risk of heart failure, and its pathogenesis involves a complex interaction between genetic and environmental factors. Variations in myosin affect contractile abilities of cardiomyocytes and cause structural and functional abnormalities in myocardium. The study aim...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/9/1554 |
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| author | Memoona Yousaf Waqas Ahmed Khan Khurrum Shahzad Haq Nawaz Khan Basharat Ali Misbah Hussain Fazli Rabbi Awan Hamid Mustafa Farah Nadia Sheikh |
| author_facet | Memoona Yousaf Waqas Ahmed Khan Khurrum Shahzad Haq Nawaz Khan Basharat Ali Misbah Hussain Fazli Rabbi Awan Hamid Mustafa Farah Nadia Sheikh |
| author_sort | Memoona Yousaf |
| collection | DOAJ |
| description | Cardiac dysfunction accelerates the risk of heart failure, and its pathogenesis involves a complex interaction between genetic and environmental factors. Variations in myosin affect contractile abilities of cardiomyocytes and cause structural and functional abnormalities in myocardium. The study aims to find the association of <i>MYH7</i> rs121913642 (c.1594 T>C) and rs121913645 (c.667G>A) variants with cardiac dysfunction in the Punjabi Pakistani population. Patients with heart failure (<i>n</i> = 232) and healthy controls (<i>n</i> = 205) were enrolled in this study. <i>MYH7</i> variant genotyping was performed using tetra ARMS-PCR. <i>MYH7</i> rs121913642 TC genotype was significantly more prevalent in the patient group (<i>p</i> < 0.001). However, <i>MYH7</i> rs121913645 genotype frequencies were not significantly different between the patient and control groups (<i>p</i> < 0.666). Regression analysis also revealed that the rs121913642 C allele increases the risk of cardiac failure by ~2 [OR:1.98, CI: 1.31–2.98, <i>p</i> < 0.001] in comparison to the T allele. High levels of the cardiac enzymes cardiac troponin I (cTnI) and CK-MB were observed in patients. There was also an increase in total cholesterol, LDL cholesterol, and uric acid in patients compared to the healthy control group (<i>p</i> < 0.001). In conclusion, the <i>MYH7</i> gene variant rs121913642 is genetically associated with cardiac dysfunction and involved in the pathogenesis of HF. |
| first_indexed | 2024-03-09T23:56:14Z |
| format | Article |
| id | doaj.art-2dcacbce832e48de93f95a6ef30921dd |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:56:14Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-2dcacbce832e48de93f95a6ef30921dd2023-11-23T16:24:04ZengMDPI AGGenes2073-44252022-08-01139155410.3390/genes13091554Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac DysfunctionMemoona Yousaf0Waqas Ahmed Khan1Khurrum Shahzad2Haq Nawaz Khan3Basharat Ali4Misbah Hussain5Fazli Rabbi Awan6Hamid Mustafa7Farah Nadia Sheikh8Department of Biotechnology, University of Sargodha, Sargodha 40100, PakistanDepartment of Biotechnology, University of Sargodha, Sargodha 40100, PakistanDepartment of Biotechnology, University of Sargodha, Sargodha 40100, PakistanDiabetes and Cardio-Metabolic Disorders Laboratory, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad 38000, PakistanDepartment of Family Medicine, University of Health Sciences, Lahore 42000, PakistanDepartment of Biotechnology, University of Sargodha, Sargodha 40100, PakistanDiabetes and Cardio-Metabolic Disorders Laboratory, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad 38000, PakistanDepartment of Animal Breeding & Genetics, University of Veterinary and Animal Sciences, Lahore 42000, PakistanServices Hospital, Jail Road, Lahore 42000, PakistanCardiac dysfunction accelerates the risk of heart failure, and its pathogenesis involves a complex interaction between genetic and environmental factors. Variations in myosin affect contractile abilities of cardiomyocytes and cause structural and functional abnormalities in myocardium. The study aims to find the association of <i>MYH7</i> rs121913642 (c.1594 T>C) and rs121913645 (c.667G>A) variants with cardiac dysfunction in the Punjabi Pakistani population. Patients with heart failure (<i>n</i> = 232) and healthy controls (<i>n</i> = 205) were enrolled in this study. <i>MYH7</i> variant genotyping was performed using tetra ARMS-PCR. <i>MYH7</i> rs121913642 TC genotype was significantly more prevalent in the patient group (<i>p</i> < 0.001). However, <i>MYH7</i> rs121913645 genotype frequencies were not significantly different between the patient and control groups (<i>p</i> < 0.666). Regression analysis also revealed that the rs121913642 C allele increases the risk of cardiac failure by ~2 [OR:1.98, CI: 1.31–2.98, <i>p</i> < 0.001] in comparison to the T allele. High levels of the cardiac enzymes cardiac troponin I (cTnI) and CK-MB were observed in patients. There was also an increase in total cholesterol, LDL cholesterol, and uric acid in patients compared to the healthy control group (<i>p</i> < 0.001). In conclusion, the <i>MYH7</i> gene variant rs121913642 is genetically associated with cardiac dysfunction and involved in the pathogenesis of HF.https://www.mdpi.com/2073-4425/13/9/1554cardiac dysfunction<i>MYH7</i>variantsrs121913642heart failure (HF) |
| spellingShingle | Memoona Yousaf Waqas Ahmed Khan Khurrum Shahzad Haq Nawaz Khan Basharat Ali Misbah Hussain Fazli Rabbi Awan Hamid Mustafa Farah Nadia Sheikh Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction Genes cardiac dysfunction <i>MYH7</i> variants rs121913642 heart failure (HF) |
| title | Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction |
| title_full | Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction |
| title_fullStr | Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction |
| title_full_unstemmed | Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction |
| title_short | Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction |
| title_sort | genetic association of beta myosin heavy chain gene myh7 with cardiac dysfunction |
| topic | cardiac dysfunction <i>MYH7</i> variants rs121913642 heart failure (HF) |
| url | https://www.mdpi.com/2073-4425/13/9/1554 |
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