Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.)
Plant growth promoting rhizobacteria (PGPR) are a group of useful bacteria that colonize the plant roots and significantly enhances the plant growth promotion. Keeping this in mind, an investigation was performed for the screening of potent PGPR strains for enhancing seed germination and vigour inde...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2022-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/molecular-identification-and-characterization-of-plant-growth-promoting-rhizobacteria-and-their-effect-on-seed-germination-and-vigour-index-of-barley-hordeum-vulgare-l/ |
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| author | Kumari Divyanshu Mukesh Yadav Vaishali Shukla Sunil Kumar Yashoda Nandan Tripathi Ram Sanmukh Upadhyay |
| author_facet | Kumari Divyanshu Mukesh Yadav Vaishali Shukla Sunil Kumar Yashoda Nandan Tripathi Ram Sanmukh Upadhyay |
| author_sort | Kumari Divyanshu |
| collection | DOAJ |
| description | Plant growth promoting rhizobacteria (PGPR) are a group of useful bacteria that colonize the plant roots and significantly enhances the plant growth promotion. Keeping this in mind, an investigation was performed for the screening of potent PGPR strains for enhancing seed germination and vigour index of Hordeum vulgare (commonly called barley). Rhizobacterial strains were isolated and screened for various plant growth promoting traits, their effect on seed germination and vigour index of barley plant through pot trial, and resistant ability under various temperature and pH range. Based upon 16S rRNA sequencing data, Pseudomonas punonensis LMT03 (R1), Pseudomonas plecoglossicida R4, Pseudomonas aeruginosa DSM50071 (R2) and Alcaligenes faecalis (DBHU5) isolates were selected and showed positive result for IAA production, Phosphate solubilization, ammonia production, catalase activity, siderophore production and MR-VP test. Barley plants treated with P. punonensis and P. plecoglossicida both showed 94.44% of highest seed germination %, while P. aeruginosa and A. faecalis showed 83.11% and 77.33% respectively in comparison to the control plant shows which 49.99% seed germination only. These respective isolates also showed 2.57, 2.37, 2.0 and 1.69 fold of increase in vigour index as compare to the control plants. The above increase in fold in vigour index and seed germination is much higher as compared to earlier reports. Collectively, the data of current study underpin that addition of these PGPRs to barley rhizosphere appears a promising strategy to enhance root and shoot biomass of this important agriculture crop. To the best of our knowledge this is the first report demonstrating the effect of P. punonensis and P. plecoglossicda on barley crop. |
| first_indexed | 2024-12-11T05:51:11Z |
| format | Article |
| id | doaj.art-2dcbe39e4b194f2890bd7a792a781d5f |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-11T05:51:11Z |
| publishDate | 2022-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
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| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-2dcbe39e4b194f2890bd7a792a781d5f2022-12-22T01:18:49ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-06-01162974989https://doi.org/10.22207/JPAM.16.2.21Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.)Kumari Divyanshuhttps://orcid.org/0000-0002-5934-257XMukesh Yadavhttps://orcid.org/0000-0003-2197-4413Vaishali Shuklahttps://orcid.org/0000-0002-7740-7015Sunil Kumarhttps://orcid.org/0000-0001-8784-1627Yashoda Nandan Tripathihttps://orcid.org/0000-0002-2974-2679Ram Sanmukh Upadhyayhttps://orcid.org/0000-0001-9738-0465Plant growth promoting rhizobacteria (PGPR) are a group of useful bacteria that colonize the plant roots and significantly enhances the plant growth promotion. Keeping this in mind, an investigation was performed for the screening of potent PGPR strains for enhancing seed germination and vigour index of Hordeum vulgare (commonly called barley). Rhizobacterial strains were isolated and screened for various plant growth promoting traits, their effect on seed germination and vigour index of barley plant through pot trial, and resistant ability under various temperature and pH range. Based upon 16S rRNA sequencing data, Pseudomonas punonensis LMT03 (R1), Pseudomonas plecoglossicida R4, Pseudomonas aeruginosa DSM50071 (R2) and Alcaligenes faecalis (DBHU5) isolates were selected and showed positive result for IAA production, Phosphate solubilization, ammonia production, catalase activity, siderophore production and MR-VP test. Barley plants treated with P. punonensis and P. plecoglossicida both showed 94.44% of highest seed germination %, while P. aeruginosa and A. faecalis showed 83.11% and 77.33% respectively in comparison to the control plant shows which 49.99% seed germination only. These respective isolates also showed 2.57, 2.37, 2.0 and 1.69 fold of increase in vigour index as compare to the control plants. The above increase in fold in vigour index and seed germination is much higher as compared to earlier reports. Collectively, the data of current study underpin that addition of these PGPRs to barley rhizosphere appears a promising strategy to enhance root and shoot biomass of this important agriculture crop. To the best of our knowledge this is the first report demonstrating the effect of P. punonensis and P. plecoglossicda on barley crop.https://microbiologyjournal.org/molecular-identification-and-characterization-of-plant-growth-promoting-rhizobacteria-and-their-effect-on-seed-germination-and-vigour-index-of-barley-hordeum-vulgare-l/bioprimingpgpr characterizationphosphate solubilizationrhizobacteriaseed germinationvigour index |
| spellingShingle | Kumari Divyanshu Mukesh Yadav Vaishali Shukla Sunil Kumar Yashoda Nandan Tripathi Ram Sanmukh Upadhyay Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.) Journal of Pure and Applied Microbiology biopriming pgpr characterization phosphate solubilization rhizobacteria seed germination vigour index |
| title | Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.) |
| title_full | Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.) |
| title_fullStr | Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.) |
| title_full_unstemmed | Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.) |
| title_short | Molecular Identification and Characterization of Plant Growth Promoting Rhizobacteria and their Effect on Seed Germination and Vigour Index of Barley (Hordeum vulgare L.) |
| title_sort | molecular identification and characterization of plant growth promoting rhizobacteria and their effect on seed germination and vigour index of barley hordeum vulgare l |
| topic | biopriming pgpr characterization phosphate solubilization rhizobacteria seed germination vigour index |
| url | https://microbiologyjournal.org/molecular-identification-and-characterization-of-plant-growth-promoting-rhizobacteria-and-their-effect-on-seed-germination-and-vigour-index-of-barley-hordeum-vulgare-l/ |
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