High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests
Microbial communities in the immediate environment of socialized invertebrates can help to suppress pathogens, in part by synthesizing bioactive natural products. Here we characterized the core microbiomes of three termite species (genus Coptotermes) and their nest material to gain more insight into...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.597628/full |
| _version_ | 1818407982796374016 |
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| author | Markus Oberpaul Celine M. Zumkeller Tanja Culver Marius Spohn Sanja Mihajlovic Benedikt Leis Stefanie P. Glaeser Rudy Plarre Dino P. McMahon Dino P. McMahon Peter Hammann Till F. Schäberle Till F. Schäberle Jens Glaeser Andreas Vilcinskas Andreas Vilcinskas |
| author_facet | Markus Oberpaul Celine M. Zumkeller Tanja Culver Marius Spohn Sanja Mihajlovic Benedikt Leis Stefanie P. Glaeser Rudy Plarre Dino P. McMahon Dino P. McMahon Peter Hammann Till F. Schäberle Till F. Schäberle Jens Glaeser Andreas Vilcinskas Andreas Vilcinskas |
| author_sort | Markus Oberpaul |
| collection | DOAJ |
| description | Microbial communities in the immediate environment of socialized invertebrates can help to suppress pathogens, in part by synthesizing bioactive natural products. Here we characterized the core microbiomes of three termite species (genus Coptotermes) and their nest material to gain more insight into the diversity of termite-associated bacteria. Sampling a healthy termite colony over time implicated a consolidated and highly stable microbiome, pointing toward the fact that beneficial bacterial phyla play a major role in termite fitness. In contrast, there was a significant shift in the composition of the core microbiome in one nest during a fungal infection, affecting the abundance of well-characterized Streptomyces species (phylum Actinobacteria) as well as less-studied bacterial phyla such as Acidobacteria. High-throughput cultivation in microplates was implemented to isolate and identify these less-studied bacterial phylogenetic group. Amplicon sequencing confirmed that our method maintained the bacterial diversity of the environmental samples, enabling the isolation of novel Acidobacteriaceae and expanding the list of cultivated species to include two strains that may define new species within the genera Terracidiphilus and Acidobacterium. |
| first_indexed | 2024-12-14T09:36:29Z |
| format | Article |
| id | doaj.art-2dcfdf1c8c4f4b15882b513dc9e01d16 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T09:36:29Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2dcfdf1c8c4f4b15882b513dc9e01d162022-12-21T23:07:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.597628597628High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite NestsMarkus Oberpaul0Celine M. Zumkeller1Tanja Culver2Marius Spohn3Sanja Mihajlovic4Benedikt Leis5Stefanie P. Glaeser6Rudy Plarre7Dino P. McMahon8Dino P. McMahon9Peter Hammann10Till F. Schäberle11Till F. Schäberle12Jens Glaeser13Andreas Vilcinskas14Andreas Vilcinskas15Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyInstitute of Applied Microbiology, Justus Liebig University Giessen, Giessen, GermanyBundesanstalt für Materialforschung und -prüfung, Berlin, GermanyBundesanstalt für Materialforschung und -prüfung, Berlin, GermanyInstitute of Biology, Free University of Berlin, Berlin, GermanySanofi-Aventis Deutschland GmbH, R&D Integrated Drug Discovery, Hoechst Industrial Park, Frankfurt am Main, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyInstitute for Insect Biotechnology, Justus Liebig University Giessen, Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, GermanyInstitute for Insect Biotechnology, Justus Liebig University Giessen, Giessen, GermanyMicrobial communities in the immediate environment of socialized invertebrates can help to suppress pathogens, in part by synthesizing bioactive natural products. Here we characterized the core microbiomes of three termite species (genus Coptotermes) and their nest material to gain more insight into the diversity of termite-associated bacteria. Sampling a healthy termite colony over time implicated a consolidated and highly stable microbiome, pointing toward the fact that beneficial bacterial phyla play a major role in termite fitness. In contrast, there was a significant shift in the composition of the core microbiome in one nest during a fungal infection, affecting the abundance of well-characterized Streptomyces species (phylum Actinobacteria) as well as less-studied bacterial phyla such as Acidobacteria. High-throughput cultivation in microplates was implemented to isolate and identify these less-studied bacterial phylogenetic group. Amplicon sequencing confirmed that our method maintained the bacterial diversity of the environmental samples, enabling the isolation of novel Acidobacteriaceae and expanding the list of cultivated species to include two strains that may define new species within the genera Terracidiphilus and Acidobacterium.https://www.frontiersin.org/articles/10.3389/fmicb.2020.597628/fulltermitesCoptotermescore microbiomenatural products discoveryAcidobacteriaunderexplored phyla |
| spellingShingle | Markus Oberpaul Celine M. Zumkeller Tanja Culver Marius Spohn Sanja Mihajlovic Benedikt Leis Stefanie P. Glaeser Rudy Plarre Dino P. McMahon Dino P. McMahon Peter Hammann Till F. Schäberle Till F. Schäberle Jens Glaeser Andreas Vilcinskas Andreas Vilcinskas High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests Frontiers in Microbiology termites Coptotermes core microbiome natural products discovery Acidobacteria underexplored phyla |
| title | High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests |
| title_full | High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests |
| title_fullStr | High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests |
| title_full_unstemmed | High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests |
| title_short | High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests |
| title_sort | high throughput cultivation for the selective isolation of acidobacteria from termite nests |
| topic | termites Coptotermes core microbiome natural products discovery Acidobacteria underexplored phyla |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.597628/full |
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