Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts
Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholestero...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2022-11-01
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| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e60 |
| _version_ | 1811188077949878272 |
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| author | Ziyi Pan Guoqing Du Guoyu Li Dongsheng Wu Xingyong Chen Zhaoyu Geng |
| author_facet | Ziyi Pan Guoqing Du Guoyu Li Dongsheng Wu Xingyong Chen Zhaoyu Geng |
| author_sort | Ziyi Pan |
| collection | DOAJ |
| description | Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway. This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction. |
| first_indexed | 2024-04-11T14:13:16Z |
| format | Article |
| id | doaj.art-2dd12c4867a74a5da48a3b49010a1f9e |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-04-11T14:13:16Z |
| publishDate | 2022-11-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-2dd12c4867a74a5da48a3b49010a1f9e2022-12-22T04:19:37ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912022-11-016461199121410.5187/jast.2022.e60Apolipoprotein H: a novel regulator of fat accumulation in duck myoblastsZiyi Pan0Guoqing Du1Guoyu Li2Dongsheng Wu3Xingyong Chen4Zhaoyu Geng5College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaApolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway. This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e60Apolipoprotein HMyoblastsLipid metabolismAKT/AMPK signalling pathway |
| spellingShingle | Ziyi Pan Guoqing Du Guoyu Li Dongsheng Wu Xingyong Chen Zhaoyu Geng Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts Journal of Animal Science and Technology Apolipoprotein H Myoblasts Lipid metabolism AKT/AMPK signalling pathway |
| title | Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts |
| title_full | Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts |
| title_fullStr | Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts |
| title_full_unstemmed | Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts |
| title_short | Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts |
| title_sort | apolipoprotein h a novel regulator of fat accumulation in duck myoblasts |
| topic | Apolipoprotein H Myoblasts Lipid metabolism AKT/AMPK signalling pathway |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e60 |
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