Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress
Stress is the physical and psychological tension felt by an individual while adapting to difficult situations. Stress is known to alter the expression of stress hormones and cause neuroinflammation in the brain. In this study, miRNAs in serum-derived neuronal exosomes (nEVs) were analyzed to determi...
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MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/18/9960 |
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| author | Minkyoung Sung Soo-Eun Sung Kyung-Ku Kang Joo-Hee Choi Sijoon Lee KilSoo Kim Ju-Hyeon Lim Gun Woo Lee Hyo-Deog Rim Byung-Soo Kim Seunghee Won Kyungmin Kim Seoyoung Jang Min-Soo Seo Jungmin Woo |
| author_facet | Minkyoung Sung Soo-Eun Sung Kyung-Ku Kang Joo-Hee Choi Sijoon Lee KilSoo Kim Ju-Hyeon Lim Gun Woo Lee Hyo-Deog Rim Byung-Soo Kim Seunghee Won Kyungmin Kim Seoyoung Jang Min-Soo Seo Jungmin Woo |
| author_sort | Minkyoung Sung |
| collection | DOAJ |
| description | Stress is the physical and psychological tension felt by an individual while adapting to difficult situations. Stress is known to alter the expression of stress hormones and cause neuroinflammation in the brain. In this study, miRNAs in serum-derived neuronal exosomes (nEVs) were analyzed to determine whether differentially expressed miRNAs could be used as biomarkers of acute stress. Specifically, acute severe stress was induced in Sprague-Dawley rats via electric foot-shock treatment. In this acute severe-stress model, time-dependent changes in the expression levels of stress hormones and neuroinflammation-related markers were analyzed. In addition, nEVs were isolated from the serum of control mice and stressed mice at various time points to determine when brain damage was most prominent; this was found to be 7 days after foot shock. Next-generation sequencing was performed to compare neuronal exosomal miRNA at day 7 with the neuronal exosomal miRNA of the control group. From this analysis, 13 upregulated and 11 downregulated miRNAs were detected. These results show that specific miRNAs are differentially expressed in nEVs from an acute severe-stress animal model. Thus, this study provides novel insights into potential stress-related biomarkers. |
| first_indexed | 2024-03-10T07:35:32Z |
| format | Article |
| id | doaj.art-2dd1612892044b03903b821f2e2c05ce |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T07:35:32Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2dd1612892044b03903b821f2e2c05ce2023-11-22T13:30:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-012218996010.3390/ijms22189960Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe StressMinkyoung Sung0Soo-Eun Sung1Kyung-Ku Kang2Joo-Hee Choi3Sijoon Lee4KilSoo Kim5Ju-Hyeon Lim6Gun Woo Lee7Hyo-Deog Rim8Byung-Soo Kim9Seunghee Won10Kyungmin Kim11Seoyoung Jang12Min-Soo Seo13Jungmin Woo14Department of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, KoreaDepartment of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, KoreaDepartment of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, KoreaDepartment of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, KoreaDepartment of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, KoreaDepartment of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, KoreaNew Drug Development Center, Osong Medical Innovation Foundation, Chungbuk 28160, KoreaDepartment of Orthopedic Surgery, Yeungnam University College of Medicine, Yeungnam University Medical Center, 170 Hyonchung-ro, Namgu, Daegu 42415, KoreaDepartment of Psychiatry, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Psychiatry, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Psychiatry, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Psychiatry, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Psychiatry, School of Medicine, Kyungpook National University, Daegu 41944, KoreaDepartment of Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, KoreaDepartment of Psychiatry, School of Medicine, Kyungpook National University, Daegu 41944, KoreaStress is the physical and psychological tension felt by an individual while adapting to difficult situations. Stress is known to alter the expression of stress hormones and cause neuroinflammation in the brain. In this study, miRNAs in serum-derived neuronal exosomes (nEVs) were analyzed to determine whether differentially expressed miRNAs could be used as biomarkers of acute stress. Specifically, acute severe stress was induced in Sprague-Dawley rats via electric foot-shock treatment. In this acute severe-stress model, time-dependent changes in the expression levels of stress hormones and neuroinflammation-related markers were analyzed. In addition, nEVs were isolated from the serum of control mice and stressed mice at various time points to determine when brain damage was most prominent; this was found to be 7 days after foot shock. Next-generation sequencing was performed to compare neuronal exosomal miRNA at day 7 with the neuronal exosomal miRNA of the control group. From this analysis, 13 upregulated and 11 downregulated miRNAs were detected. These results show that specific miRNAs are differentially expressed in nEVs from an acute severe-stress animal model. Thus, this study provides novel insights into potential stress-related biomarkers.https://www.mdpi.com/1422-0067/22/18/9960acute severe stressneuroinflammationexosomesmiRNAbiomarkers |
| spellingShingle | Minkyoung Sung Soo-Eun Sung Kyung-Ku Kang Joo-Hee Choi Sijoon Lee KilSoo Kim Ju-Hyeon Lim Gun Woo Lee Hyo-Deog Rim Byung-Soo Kim Seunghee Won Kyungmin Kim Seoyoung Jang Min-Soo Seo Jungmin Woo Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress International Journal of Molecular Sciences acute severe stress neuroinflammation exosomes miRNA biomarkers |
| title | Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress |
| title_full | Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress |
| title_fullStr | Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress |
| title_full_unstemmed | Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress |
| title_short | Serum-Derived Neuronal Exosomal miRNAs as Biomarkers of Acute Severe Stress |
| title_sort | serum derived neuronal exosomal mirnas as biomarkers of acute severe stress |
| topic | acute severe stress neuroinflammation exosomes miRNA biomarkers |
| url | https://www.mdpi.com/1422-0067/22/18/9960 |
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