Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model
Abstract This study aimed to evaluate the efficacy of calcium carbonate nanoparticles (CCNPs) to induce new bone formation in a critical size segmental bone defect in rabbit’s radius when used alone, combined with silver nanoparticles (AgNPs) as a paste, or as a composite containing CCNPs, AgNPs, an...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42292-x |
| _version_ | 1797559817913499648 |
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| author | Mohamed Abd-Elkawi Ahmed Sharshar Tarek Misk Islam Elgohary Shaaban Gadallah |
| author_facet | Mohamed Abd-Elkawi Ahmed Sharshar Tarek Misk Islam Elgohary Shaaban Gadallah |
| author_sort | Mohamed Abd-Elkawi |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the efficacy of calcium carbonate nanoparticles (CCNPs) to induce new bone formation in a critical size segmental bone defect in rabbit’s radius when used alone, combined with silver nanoparticles (AgNPs) as a paste, or as a composite containing CCNPs, AgNPs, and advanced platelet-rich fibrin (A-PRF). Thirty-six adult apparently healthy male New Zealand White rabbits aging from 5 to 6 months and weighting 3.5 ± 0.5 kg were used. The animals were divided into four groups; control group, CCNPs group, CCNPs/AgNPs paste group, and CCNPs/AgNPs/A-PRF composite group. The animals were investigated at 4, 8, and 12 weeks post-implantation in which the healing was evaluated using computed tomographic (CT) and histopathological evaluation. The results revealed that CCNPs/AgNPs paste and CCNPs/AgNPs/A-PRF composite has a superior effect regarding the amount and the quality of the newly formed bone compared to the control and the CCNPs alone. In conclusion, addition of AgNPs and/or A-PRF to CCNPs has reduced its resorption rate and improved its osteogenic and osteoinductive properties. |
| first_indexed | 2024-03-10T17:50:40Z |
| format | Article |
| id | doaj.art-2dd172c4e93141829bf19e1f1752e394 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:50:40Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2dd172c4e93141829bf19e1f1752e3942023-11-20T09:22:15ZengNature PortfolioScientific Reports2045-23222023-09-0113111210.1038/s41598-023-42292-xEffect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit modelMohamed Abd-Elkawi0Ahmed Sharshar1Tarek Misk2Islam Elgohary3Shaaban Gadallah4Department of Surgery, Radiology and Anesthesiology, Faculty of Veterinary Medicine, New Valley UniversityDepartment of Surgery, Radiology and Anesthesiology, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Surgery, Radiology and Anesthesiology, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Pathology, Agricultural Research Center (ARC), Animal Health Research Institute (AHRI)Department of Surgery, Radiology and Anesthesiology, Faculty of Veterinary Medicine, University of Sadat CityAbstract This study aimed to evaluate the efficacy of calcium carbonate nanoparticles (CCNPs) to induce new bone formation in a critical size segmental bone defect in rabbit’s radius when used alone, combined with silver nanoparticles (AgNPs) as a paste, or as a composite containing CCNPs, AgNPs, and advanced platelet-rich fibrin (A-PRF). Thirty-six adult apparently healthy male New Zealand White rabbits aging from 5 to 6 months and weighting 3.5 ± 0.5 kg were used. The animals were divided into four groups; control group, CCNPs group, CCNPs/AgNPs paste group, and CCNPs/AgNPs/A-PRF composite group. The animals were investigated at 4, 8, and 12 weeks post-implantation in which the healing was evaluated using computed tomographic (CT) and histopathological evaluation. The results revealed that CCNPs/AgNPs paste and CCNPs/AgNPs/A-PRF composite has a superior effect regarding the amount and the quality of the newly formed bone compared to the control and the CCNPs alone. In conclusion, addition of AgNPs and/or A-PRF to CCNPs has reduced its resorption rate and improved its osteogenic and osteoinductive properties.https://doi.org/10.1038/s41598-023-42292-x |
| spellingShingle | Mohamed Abd-Elkawi Ahmed Sharshar Tarek Misk Islam Elgohary Shaaban Gadallah Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model Scientific Reports |
| title | Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model |
| title_full | Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model |
| title_fullStr | Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model |
| title_full_unstemmed | Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model |
| title_short | Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model |
| title_sort | effect of calcium carbonate nanoparticles silver nanoparticles and advanced platelet rich fibrin for enhancing bone healing in a rabbit model |
| url | https://doi.org/10.1038/s41598-023-42292-x |
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