Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i>
A variety of organisms produce bioactive peptides that express inhibition activity against other organisms. <i>Saccharomyces cerevisiae</i> is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/3/324 |
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| author | Shayma Thyab Gddoa Al-sahlany Ammar B. Altemimi Alaa Jabbar Abd Al-Manhel Alaa Kareem Niamah Naoufal Lakhssassi Salam A. Ibrahim |
| author_facet | Shayma Thyab Gddoa Al-sahlany Ammar B. Altemimi Alaa Jabbar Abd Al-Manhel Alaa Kareem Niamah Naoufal Lakhssassi Salam A. Ibrahim |
| author_sort | Shayma Thyab Gddoa Al-sahlany |
| collection | DOAJ |
| description | A variety of organisms produce bioactive peptides that express inhibition activity against other organisms. <i>Saccharomyces cerevisiae</i> is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was produced and isolated from <i>Saccharomyces</i> <i>cerevisiae</i> (Baker’s yeast) by an ultrafiltration process (two membranes with cut-offs of 2 and 10 kDa) and purified using the ÄKTA Pure 25 system. Antibacterial peptide activity was characterized and examined against four bacterial strains including Gram-positive and Gram-negative bacteria. The optimum condition for yeast growth and antibacterial peptide production against both <i>Escherichia. coli</i> and <i>Klebsiella aerogenes</i> was 25−30 °C within a 48 h period. The isolated peptide had a molecular weight of 9770 Da, was thermostable at 50−90 °C for 30 min, and tolerated a pH range of 5−7 at 4 °C and 25 °C during the first 24 h, making this isolated antibacterial peptides suitable for use in sterilization and thermal processes, which are very important aspect in food production. The isolated antibacterial peptide caused a rapid and steady decline in the number of viable cells from 2 to 2.3 log units of gram-negative strains and from 1.5 to 1.8 log units of gram-positive strains during 24 h of incubation. The isolated antibacterial peptide from <i>Saccharomyces cerevisiae</i> may present a potential biopreservative compound in the food industry exhibiting inhibition activity against gram-negative and gram-positive bacteria. |
| first_indexed | 2024-12-22T03:16:48Z |
| format | Article |
| id | doaj.art-2dd2de04140a467da7b105b6399e5afc |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-12-22T03:16:48Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-2dd2de04140a467da7b105b6399e5afc2022-12-21T18:40:48ZengMDPI AGFoods2304-81582020-03-019332410.3390/foods9030324foods9030324Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i>Shayma Thyab Gddoa Al-sahlany0Ammar B. Altemimi1Alaa Jabbar Abd Al-Manhel2Alaa Kareem Niamah3Naoufal Lakhssassi4Salam A. Ibrahim5Department of Food Science, College of Agriculture, University of Basrah, Basrah 61004, IraqDepartment of Food Science, College of Agriculture, University of Basrah, Basrah 61004, IraqDepartment of Food Science, College of Agriculture, University of Basrah, Basrah 61004, IraqDepartment of Food Science, College of Agriculture, University of Basrah, Basrah 61004, IraqDepartment of Plant Soil and Agricultural Systems, Agriculture College, Southern Illinois University, Carbondale, IL 62901, USAFood and Nutritional Science Program, North Carolina A & T State University, Greensboro, NC 27411, USAA variety of organisms produce bioactive peptides that express inhibition activity against other organisms. <i>Saccharomyces cerevisiae</i> is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was produced and isolated from <i>Saccharomyces</i> <i>cerevisiae</i> (Baker’s yeast) by an ultrafiltration process (two membranes with cut-offs of 2 and 10 kDa) and purified using the ÄKTA Pure 25 system. Antibacterial peptide activity was characterized and examined against four bacterial strains including Gram-positive and Gram-negative bacteria. The optimum condition for yeast growth and antibacterial peptide production against both <i>Escherichia. coli</i> and <i>Klebsiella aerogenes</i> was 25−30 °C within a 48 h period. The isolated peptide had a molecular weight of 9770 Da, was thermostable at 50−90 °C for 30 min, and tolerated a pH range of 5−7 at 4 °C and 25 °C during the first 24 h, making this isolated antibacterial peptides suitable for use in sterilization and thermal processes, which are very important aspect in food production. The isolated antibacterial peptide caused a rapid and steady decline in the number of viable cells from 2 to 2.3 log units of gram-negative strains and from 1.5 to 1.8 log units of gram-positive strains during 24 h of incubation. The isolated antibacterial peptide from <i>Saccharomyces cerevisiae</i> may present a potential biopreservative compound in the food industry exhibiting inhibition activity against gram-negative and gram-positive bacteria.https://www.mdpi.com/2304-8158/9/3/324<i>saccharomyces cerevisiae</i>bioactive peptideantibacterialäkta pure |
| spellingShingle | Shayma Thyab Gddoa Al-sahlany Ammar B. Altemimi Alaa Jabbar Abd Al-Manhel Alaa Kareem Niamah Naoufal Lakhssassi Salam A. Ibrahim Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i> Foods <i>saccharomyces cerevisiae</i> bioactive peptide antibacterial äkta pure |
| title | Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i> |
| title_full | Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i> |
| title_short | Purification of Bioactive Peptide with Antimicrobial Properties Produced by <i>Saccharomyces cerevisiae</i> |
| title_sort | purification of bioactive peptide with antimicrobial properties produced by i saccharomyces cerevisiae i |
| topic | <i>saccharomyces cerevisiae</i> bioactive peptide antibacterial äkta pure |
| url | https://www.mdpi.com/2304-8158/9/3/324 |
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