Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalate
Di-(2-ethylhexyl) phthalate (DEHP) has become a ubiquitous contaminant in aquatic environments due to its increasing utilization in recent years. Although its environmental risks to aquatic organisms have attracted widespread attention, the ecotoxicological effects and toxic mechanism of DEHP on zeb...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Ecological Indicators |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1470160X22008615 |
| _version_ | 1798001133194575872 |
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| author | Xianxu Li Qian Wang Can Wang Zhongkang Yang Jinhua Wang Lusheng Zhu Dexin Zhang Jun Wang |
| author_facet | Xianxu Li Qian Wang Can Wang Zhongkang Yang Jinhua Wang Lusheng Zhu Dexin Zhang Jun Wang |
| author_sort | Xianxu Li |
| collection | DOAJ |
| description | Di-(2-ethylhexyl) phthalate (DEHP) has become a ubiquitous contaminant in aquatic environments due to its increasing utilization in recent years. Although its environmental risks to aquatic organisms have attracted widespread attention, the ecotoxicological effects and toxic mechanism of DEHP on zebrafish (Danio rerio) liver remain poorly understood. Herein, this study investigated the acute toxicity, oxidative damage, neurotoxicity, and DNA damage of DEHP on zebrafish at the individual, tissue, physiological and biochemical levels. Results showed that no evident lethal effects of DEHP on zebrafish were observed in all testing concentrations. But in the chronic toxicity test (28 days), superoxide dismutase (SOD) and catalase (CAT) activities in zebrafish liver mainly increased during the pre-and mid-exposure experiment and subsequently decreased after 28 days exposure, whereas the acetylcholinesterase (AChE) activities were always inhibited. By comparison, reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione S-transferase (GST) levels generally increased throughout the experiment, indicating that DEHP enhanced oxidative stress lipid peroxidation, and cytotoxicity of zebrafish liver. DEHP induced DNA damage in hepatocyte, which was positively correlated with changes in ROS levels (0.9874 ≥ R2 ≥ 0.7374). Based on the risk assessment results, the integrated biomarker response (IBR) values were positively correlated with the DHEP concentrations, suggesting that higher DHEP concentrations may cause much more serious detrimental effects to zebrafish liver. Overall, this study is of great significance for understanding the ecotoxicity and mechanism of DEHP on zebrafish. |
| first_indexed | 2024-04-11T11:32:17Z |
| format | Article |
| id | doaj.art-2dd4f3555e504f6089fc21c3240ff4f3 |
| institution | Directory Open Access Journal |
| issn | 1470-160X |
| language | English |
| last_indexed | 2024-04-11T11:32:17Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecological Indicators |
| spelling | doaj.art-2dd4f3555e504f6089fc21c3240ff4f32022-12-22T04:26:06ZengElsevierEcological Indicators1470-160X2022-10-01143109388Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalateXianxu Li0Qian Wang1Can Wang2Zhongkang Yang3Jinhua Wang4Lusheng Zhu5Dexin Zhang6Jun Wang7College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai′an 271000, ChinaCollege of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai′an 271000, ChinaCollege of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai′an 271000, ChinaCollege of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai′an 271000, ChinaCollege of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai′an 271000, ChinaCollege of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai′an 271000, ChinaBureau of Agriculture and Rural Affairs of Changle, Shandong Province, Changle 262400, ChinaCollege of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai′an 271000, China; Corresponding author at: College of Resources and Environment, Shandong Agricultural University, 61 Daizong Road, Taian 271018, China.Di-(2-ethylhexyl) phthalate (DEHP) has become a ubiquitous contaminant in aquatic environments due to its increasing utilization in recent years. Although its environmental risks to aquatic organisms have attracted widespread attention, the ecotoxicological effects and toxic mechanism of DEHP on zebrafish (Danio rerio) liver remain poorly understood. Herein, this study investigated the acute toxicity, oxidative damage, neurotoxicity, and DNA damage of DEHP on zebrafish at the individual, tissue, physiological and biochemical levels. Results showed that no evident lethal effects of DEHP on zebrafish were observed in all testing concentrations. But in the chronic toxicity test (28 days), superoxide dismutase (SOD) and catalase (CAT) activities in zebrafish liver mainly increased during the pre-and mid-exposure experiment and subsequently decreased after 28 days exposure, whereas the acetylcholinesterase (AChE) activities were always inhibited. By comparison, reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione S-transferase (GST) levels generally increased throughout the experiment, indicating that DEHP enhanced oxidative stress lipid peroxidation, and cytotoxicity of zebrafish liver. DEHP induced DNA damage in hepatocyte, which was positively correlated with changes in ROS levels (0.9874 ≥ R2 ≥ 0.7374). Based on the risk assessment results, the integrated biomarker response (IBR) values were positively correlated with the DHEP concentrations, suggesting that higher DHEP concentrations may cause much more serious detrimental effects to zebrafish liver. Overall, this study is of great significance for understanding the ecotoxicity and mechanism of DEHP on zebrafish.http://www.sciencedirect.com/science/article/pii/S1470160X22008615Di-(2-ethylhexyl) phthalate (DEHP)Zebrafish (Danio rerio)Reactive oxygen species (ROS)Oxidative stressDNA damageIntegrated Biomarker Response (IBR) |
| spellingShingle | Xianxu Li Qian Wang Can Wang Zhongkang Yang Jinhua Wang Lusheng Zhu Dexin Zhang Jun Wang Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalate Ecological Indicators Di-(2-ethylhexyl) phthalate (DEHP) Zebrafish (Danio rerio) Reactive oxygen species (ROS) Oxidative stress DNA damage Integrated Biomarker Response (IBR) |
| title | Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalate |
| title_full | Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalate |
| title_fullStr | Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalate |
| title_full_unstemmed | Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalate |
| title_short | Ecotoxicological response of zebrafish liver (Danio rerio) induced by di-(2-ethylhexyl) phthalate |
| title_sort | ecotoxicological response of zebrafish liver danio rerio induced by di 2 ethylhexyl phthalate |
| topic | Di-(2-ethylhexyl) phthalate (DEHP) Zebrafish (Danio rerio) Reactive oxygen species (ROS) Oxidative stress DNA damage Integrated Biomarker Response (IBR) |
| url | http://www.sciencedirect.com/science/article/pii/S1470160X22008615 |
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