Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm
<i>Pleurotus ostreatus</i> (Jacq.) P. Kumm is the second most cultivated edible mushroom worldwide. Temperature is one of the main environmental factors affecting the growth and fructification of mushrooms. However, the effects of ambient temperature on the growth, development, and produ...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/1/95 |
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| author | Yanru Hu Fangfang Xue Yujie Chen Yuancheng Qi Wei Zhu Fengqin Wang Qing Wen Jinwen Shen |
| author_facet | Yanru Hu Fangfang Xue Yujie Chen Yuancheng Qi Wei Zhu Fengqin Wang Qing Wen Jinwen Shen |
| author_sort | Yanru Hu |
| collection | DOAJ |
| description | <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm is the second most cultivated edible mushroom worldwide. Temperature is one of the main environmental factors affecting the growth and fructification of mushrooms. However, the effects of ambient temperature on the growth, development, and production of bag-cultured <i>P. ostreatus</i> are still unclear. In this study, eight ambient temperatures (15 °C, 18 °C, 20 °C, 22 °C, 25 °C, 28 °C, 30 °C, and 32 °C) were used to cultivate <i>P. ostreatus</i> mycelia with cottonseed hull as substrate. Then, the agronomic traits and the activities of substrate-degrading and antioxidant enzymes were determined. The mycelial growth and fructification of <i>P. ostreatus</i> differed significantly under different temperatures, and 22 °C was the optimum temperature for mycelial culture when we comprehensively analyzed all the agronomic traits. The mycelial growth rate and the biological efficiency under 22 °C were 0.56 cm/d and 99.53%, respectively. Furthermore, there were also significant differences in the activities of substrate-degrading enzymes and antioxidant enzymes under different ambient temperatures. The highest activities of lignocellulose-degrading enzymes (laccase, xylanase, and cellulase), amylase, and proteinase at the vegetative growth stage occurred at 15–22 °C, 20–22 °C, and 22–30 °C, respectively. The highest activities of superoxide dismutase, peroxidase, and catalase at the vegetative growth stage occurred at 28–30 °C. This study provides a reference temperature for bag-cultured <i>P. ostreatus</i>. |
| first_indexed | 2024-03-09T12:29:27Z |
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| language | English |
| last_indexed | 2024-03-09T12:29:27Z |
| publishDate | 2023-01-01 |
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| series | Horticulturae |
| spelling | doaj.art-2dd6aa7238414e2d8bb9ee781fc057032023-11-30T22:30:51ZengMDPI AGHorticulturae2311-75242023-01-01919510.3390/horticulturae9010095Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. KummYanru Hu0Fangfang Xue1Yujie Chen2Yuancheng Qi3Wei Zhu4Fengqin Wang5Qing Wen6Jinwen Shen7Key Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaXinyang Academy of Agricultural Sciences, Xinyang 464000, ChinaKey Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, ChinaKey Laboratory of Agricultural Microbial Enzyme Engineering, Ministry of Agriculture, Rural Department, College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China<i>Pleurotus ostreatus</i> (Jacq.) P. Kumm is the second most cultivated edible mushroom worldwide. Temperature is one of the main environmental factors affecting the growth and fructification of mushrooms. However, the effects of ambient temperature on the growth, development, and production of bag-cultured <i>P. ostreatus</i> are still unclear. In this study, eight ambient temperatures (15 °C, 18 °C, 20 °C, 22 °C, 25 °C, 28 °C, 30 °C, and 32 °C) were used to cultivate <i>P. ostreatus</i> mycelia with cottonseed hull as substrate. Then, the agronomic traits and the activities of substrate-degrading and antioxidant enzymes were determined. The mycelial growth and fructification of <i>P. ostreatus</i> differed significantly under different temperatures, and 22 °C was the optimum temperature for mycelial culture when we comprehensively analyzed all the agronomic traits. The mycelial growth rate and the biological efficiency under 22 °C were 0.56 cm/d and 99.53%, respectively. Furthermore, there were also significant differences in the activities of substrate-degrading enzymes and antioxidant enzymes under different ambient temperatures. The highest activities of lignocellulose-degrading enzymes (laccase, xylanase, and cellulase), amylase, and proteinase at the vegetative growth stage occurred at 15–22 °C, 20–22 °C, and 22–30 °C, respectively. The highest activities of superoxide dismutase, peroxidase, and catalase at the vegetative growth stage occurred at 28–30 °C. This study provides a reference temperature for bag-cultured <i>P. ostreatus</i>.https://www.mdpi.com/2311-7524/9/1/95<i>Pleurotus ostreatus</i>temperaturemycelial growthbiological efficiencysubstrate-degrading enzymeantioxidant enzyme |
| spellingShingle | Yanru Hu Fangfang Xue Yujie Chen Yuancheng Qi Wei Zhu Fengqin Wang Qing Wen Jinwen Shen Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm Horticulturae <i>Pleurotus ostreatus</i> temperature mycelial growth biological efficiency substrate-degrading enzyme antioxidant enzyme |
| title | Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm |
| title_full | Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm |
| title_fullStr | Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm |
| title_full_unstemmed | Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm |
| title_short | Effects and Mechanism of the Mycelial Culture Temperature on the Growth and Development of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm |
| title_sort | effects and mechanism of the mycelial culture temperature on the growth and development of i pleurotus ostreatus i jacq p kumm |
| topic | <i>Pleurotus ostreatus</i> temperature mycelial growth biological efficiency substrate-degrading enzyme antioxidant enzyme |
| url | https://www.mdpi.com/2311-7524/9/1/95 |
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