Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs
The laser treatment and hydroxyapatite coating of dental implants are supposed to enhance osseointegration, but prior to preclinical testing, any negative impact on cell viability should be ruled out. This study aimed to evaluate the response of murine osteogenic cell lineage MC3T3-E1 and the bone m...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/13/23/12646 |
| _version_ | 1797400440387665920 |
|---|---|
| author | Ana Flávia Piquera Santos Lara Cristina Cunha Cervantes Roberta Okamoto Antonio Carlos Guastaldi Thallita Pereira Queiroz Layla Panahipour Reinhard Gruber Francisley Ávila Souza |
| author_facet | Ana Flávia Piquera Santos Lara Cristina Cunha Cervantes Roberta Okamoto Antonio Carlos Guastaldi Thallita Pereira Queiroz Layla Panahipour Reinhard Gruber Francisley Ávila Souza |
| author_sort | Ana Flávia Piquera Santos |
| collection | DOAJ |
| description | The laser treatment and hydroxyapatite coating of dental implants are supposed to enhance osseointegration, but prior to preclinical testing, any negative impact on cell viability should be ruled out. This study aimed to evaluate the response of murine osteogenic cell lineage MC3T3-E1 and the bone marrow-derived stromal cells ST2 to surface modifications of machined titanium discs, e.g., laser treatment without and with hydroxyapatite coating, as well as sandblasting followed by acid etching. Scanning electron microscopy and the contact angle measurements revealed that laser treatment caused a honeycomb surface and higher wettability compared to a machined or sandblasting acid-etched surface. Hydroxyapatite coating, however, not only reduced the viability of MC3T3-E1 and ST2 cells but also provoked the expression and release of interleukin-6. These findings suggest that the laser treatment of titanium supports its hydrophilicity, but adding hydroxyapatite can reduce cell viability and induce the concomitant release of inflammatory cytokines. |
| first_indexed | 2024-03-09T01:55:31Z |
| format | Article |
| id | doaj.art-2ddc4fa9e0ea4402993a69330d5fde64 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T01:55:31Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-2ddc4fa9e0ea4402993a69330d5fde642023-12-08T15:11:16ZengMDPI AGApplied Sciences2076-34172023-11-0113231264610.3390/app132312646Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium DiscsAna Flávia Piquera Santos0Lara Cristina Cunha Cervantes1Roberta Okamoto2Antonio Carlos Guastaldi3Thallita Pereira Queiroz4Layla Panahipour5Reinhard Gruber6Francisley Ávila Souza7Department of Oral Biology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Aracatuba 16015-050, BrazilDepartment of Analytical, Physicochemical, and Inorganic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-060, BrazilDepartment of Health Science, University of Araraquara—UNIARA, Araraquara 14801-340, BrazilDepartment of Oral Biology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Aracatuba 16015-050, BrazilThe laser treatment and hydroxyapatite coating of dental implants are supposed to enhance osseointegration, but prior to preclinical testing, any negative impact on cell viability should be ruled out. This study aimed to evaluate the response of murine osteogenic cell lineage MC3T3-E1 and the bone marrow-derived stromal cells ST2 to surface modifications of machined titanium discs, e.g., laser treatment without and with hydroxyapatite coating, as well as sandblasting followed by acid etching. Scanning electron microscopy and the contact angle measurements revealed that laser treatment caused a honeycomb surface and higher wettability compared to a machined or sandblasting acid-etched surface. Hydroxyapatite coating, however, not only reduced the viability of MC3T3-E1 and ST2 cells but also provoked the expression and release of interleukin-6. These findings suggest that the laser treatment of titanium supports its hydrophilicity, but adding hydroxyapatite can reduce cell viability and induce the concomitant release of inflammatory cytokines.https://www.mdpi.com/2076-3417/13/23/12646biocompatible materialshydroxyapatitetitanium discsscanning electron microscopycell culture techniquesinflammation mediators |
| spellingShingle | Ana Flávia Piquera Santos Lara Cristina Cunha Cervantes Roberta Okamoto Antonio Carlos Guastaldi Thallita Pereira Queiroz Layla Panahipour Reinhard Gruber Francisley Ávila Souza Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs Applied Sciences biocompatible materials hydroxyapatite titanium discs scanning electron microscopy cell culture techniques inflammation mediators |
| title | Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs |
| title_full | Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs |
| title_fullStr | Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs |
| title_full_unstemmed | Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs |
| title_short | Interleukin-6 Expression of Osteogenic Cell Lines Grown on Laser-Treated and Hydroxyapatite-Coated Titanium Discs |
| title_sort | interleukin 6 expression of osteogenic cell lines grown on laser treated and hydroxyapatite coated titanium discs |
| topic | biocompatible materials hydroxyapatite titanium discs scanning electron microscopy cell culture techniques inflammation mediators |
| url | https://www.mdpi.com/2076-3417/13/23/12646 |
| work_keys_str_mv | AT anaflaviapiquerasantos interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs AT laracristinacunhacervantes interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs AT robertaokamoto interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs AT antoniocarlosguastaldi interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs AT thallitapereiraqueiroz interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs AT laylapanahipour interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs AT reinhardgruber interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs AT francisleyavilasouza interleukin6expressionofosteogeniccelllinesgrownonlasertreatedandhydroxyapatitecoatedtitaniumdiscs |