Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance
(1) Background: <i>Candida</i> is a genus of yeasts with notable pathogenicity and significant ability to develop antimicrobial resistance. <i>Gossypium hirsutum</i> L., a medicinal plant that is traditionally used due to its antimicrobial properties, has demonstrated signifi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/4/698 |
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| author | Luciene Ferreira de Lima Jacqueline Cosmo Andrade-Pinheiro Maria Audilene Freitas Adriely Idalina da Silva Victor Juno Alencar Fonseca Taís Gusmão da Silva Josefa Carolaine Pereira da Silva Rosilaine Honorato de Lima Débora Lima Sales Rejane Pereira Neves Edy Sousa de Brito Paulo Riceli Vasconcelos Ribeiro Kirley Marques Canuto Henrique Douglas Melo Coutinho Abolghasem Siyadatpanah Bonglee Kim Maria Flaviana Bezerra Morais-Braga |
| author_facet | Luciene Ferreira de Lima Jacqueline Cosmo Andrade-Pinheiro Maria Audilene Freitas Adriely Idalina da Silva Victor Juno Alencar Fonseca Taís Gusmão da Silva Josefa Carolaine Pereira da Silva Rosilaine Honorato de Lima Débora Lima Sales Rejane Pereira Neves Edy Sousa de Brito Paulo Riceli Vasconcelos Ribeiro Kirley Marques Canuto Henrique Douglas Melo Coutinho Abolghasem Siyadatpanah Bonglee Kim Maria Flaviana Bezerra Morais-Braga |
| author_sort | Luciene Ferreira de Lima |
| collection | DOAJ |
| description | (1) Background: <i>Candida</i> is a genus of yeasts with notable pathogenicity and significant ability to develop antimicrobial resistance. <i>Gossypium hirsutum</i> L., a medicinal plant that is traditionally used due to its antimicrobial properties, has demonstrated significant antifungal activity. Therefore, this study investigated the chemical composition and anti-<i>Candida</i> effects of aqueous (AELG) and hydroethanolic (HELG) extracts obtained from the leaves of this plant. (2) Methods: The extracts were chemically characterized by UPLC–QTOF-MS/MS, and their anti-<i>Candida</i> activities were investigated by analyzing cell viability, biofilm production, morphological transition, and enhancement of antifungal resistance. (3) Results: The UPLC–QTOF-MS/MS analysis revealed the presence of twenty-one compounds in both AELG and HELG, highlighting the predominance of flavonoids. The combination of the extracts with fluconazole significantly reduced its IC<sub>50</sub> values against <i>Candida albicans</i> INCQS 40006, <i>Candida tropicalis</i> INCQS 40042, and <i>C. tropicalis</i> URM 4262 strains, indicating enhanced antifungal activity. About biofilm production, significant inhibition was observed only for the AELG-treated <i>C. tropicalis</i> URM 4262 strain in comparison with the untreated control. Accordingly, this extract showed more significant inhibitory effects on the morphological transition of the INCQS 40006 and URM 4387 strains of <i>C. albicans</i> (4) Conclusions: <i>Gossypium hirsutum</i> L. presents promising antifungal effects, that may be potentially linked to the combined activity of chemical constituents identified in its extracts. |
| first_indexed | 2024-03-09T10:29:56Z |
| format | Article |
| id | doaj.art-2ddcc0cd3c924adda21f2e63633abc4d |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T10:29:56Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-2ddcc0cd3c924adda21f2e63633abc4d2023-12-01T21:18:24ZengMDPI AGPharmaceutics1999-49232022-03-0114469810.3390/pharmaceutics14040698Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal ResistanceLuciene Ferreira de Lima0Jacqueline Cosmo Andrade-Pinheiro1Maria Audilene Freitas2Adriely Idalina da Silva3Victor Juno Alencar Fonseca4Taís Gusmão da Silva5Josefa Carolaine Pereira da Silva6Rosilaine Honorato de Lima7Débora Lima Sales8Rejane Pereira Neves9Edy Sousa de Brito10Paulo Riceli Vasconcelos Ribeiro11Kirley Marques Canuto12Henrique Douglas Melo Coutinho13Abolghasem Siyadatpanah14Bonglee Kim15Maria Flaviana Bezerra Morais-Braga16Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, BrazilBioassay Laboratory (Labio), Federal University of Cariri (UFCA), Brejo Santo 63260-000, BrazilLaboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco (UFPE), Recife 50670-901, BrazilLaboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco (UFPE), Recife 50670-901, BrazilLaboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, BrazilLaboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, BrazilLaboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, BrazilLaboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, BrazilLaboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, BrazilLaboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco (UFPE), Recife 50670-901, BrazilLaboratory Multi-User Natural Product Chemistry (LMQPN), Embrapa Agroindústria Tropical, Fortaleza 60511-110, BrazilLaboratory Multi-User Natural Product Chemistry (LMQPN), Embrapa Agroindústria Tropical, Fortaleza 60511-110, BrazilLaboratory Multi-User Natural Product Chemistry (LMQPN), Embrapa Agroindústria Tropical, Fortaleza 60511-110, BrazilLaboratory of Microbiology and Molecular Biology (LMBM), University Regional of Cariri (URCA), Crato 63105-010, BrazilFerdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand 9717853577, IranDepartment of Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaLaboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil(1) Background: <i>Candida</i> is a genus of yeasts with notable pathogenicity and significant ability to develop antimicrobial resistance. <i>Gossypium hirsutum</i> L., a medicinal plant that is traditionally used due to its antimicrobial properties, has demonstrated significant antifungal activity. Therefore, this study investigated the chemical composition and anti-<i>Candida</i> effects of aqueous (AELG) and hydroethanolic (HELG) extracts obtained from the leaves of this plant. (2) Methods: The extracts were chemically characterized by UPLC–QTOF-MS/MS, and their anti-<i>Candida</i> activities were investigated by analyzing cell viability, biofilm production, morphological transition, and enhancement of antifungal resistance. (3) Results: The UPLC–QTOF-MS/MS analysis revealed the presence of twenty-one compounds in both AELG and HELG, highlighting the predominance of flavonoids. The combination of the extracts with fluconazole significantly reduced its IC<sub>50</sub> values against <i>Candida albicans</i> INCQS 40006, <i>Candida tropicalis</i> INCQS 40042, and <i>C. tropicalis</i> URM 4262 strains, indicating enhanced antifungal activity. About biofilm production, significant inhibition was observed only for the AELG-treated <i>C. tropicalis</i> URM 4262 strain in comparison with the untreated control. Accordingly, this extract showed more significant inhibitory effects on the morphological transition of the INCQS 40006 and URM 4387 strains of <i>C. albicans</i> (4) Conclusions: <i>Gossypium hirsutum</i> L. presents promising antifungal effects, that may be potentially linked to the combined activity of chemical constituents identified in its extracts.https://www.mdpi.com/1999-4923/14/4/698<i>Malvaceae</i>UPLC–QTOF-MS/MSbiofilm eradicationantimicrobial resistance |
| spellingShingle | Luciene Ferreira de Lima Jacqueline Cosmo Andrade-Pinheiro Maria Audilene Freitas Adriely Idalina da Silva Victor Juno Alencar Fonseca Taís Gusmão da Silva Josefa Carolaine Pereira da Silva Rosilaine Honorato de Lima Débora Lima Sales Rejane Pereira Neves Edy Sousa de Brito Paulo Riceli Vasconcelos Ribeiro Kirley Marques Canuto Henrique Douglas Melo Coutinho Abolghasem Siyadatpanah Bonglee Kim Maria Flaviana Bezerra Morais-Braga Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance Pharmaceutics <i>Malvaceae</i> UPLC–QTOF-MS/MS biofilm eradication antimicrobial resistance |
| title | Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance |
| title_full | Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance |
| title_fullStr | Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance |
| title_full_unstemmed | Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance |
| title_short | Anti-<i>Candida</i> Properties of <i>Gossypium hirsutum</i> L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance |
| title_sort | anti i candida i properties of i gossypium hirsutum i l enhancement of fungal growth biofilm production and antifungal resistance |
| topic | <i>Malvaceae</i> UPLC–QTOF-MS/MS biofilm eradication antimicrobial resistance |
| url | https://www.mdpi.com/1999-4923/14/4/698 |
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