Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil
Abstract Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with con...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2017-11-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017005027105&lng=en&tlng=en |
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| author | Ana Sheila Cypriano Gilda Alves Antonio Augusto Ornellas José Scheinkman Renata Almeida Luciano Scherrer Claudia Lage |
| author_facet | Ana Sheila Cypriano Gilda Alves Antonio Augusto Ornellas José Scheinkman Renata Almeida Luciano Scherrer Claudia Lage |
| author_sort | Ana Sheila Cypriano |
| collection | DOAJ |
| description | Abstract Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with confirmed prostate cancer and 200 unaffected men, from Rio de Janeiro, Brazil. XPD/Lys751Gln (rs13181), APEX1/Asp148Glu (rs1130409), and RAD51/G135C (rs1801320) SNPs were analyzed by PCR-RFLP. Allelic and genotypic frequencies were calculated and compared by Chi-Square test. The association between SNPs and clinical/epidemiological data was considered significant by Odds Ratio analysis, with IC95% and a p-value≤0.05. Only the XPD/Lys751Gln SNP significantly increased susceptibility to disease in southeastern Brazilian men, with p≤0.001 [OR=2.36 (1.46-3.84)], with no association with APEX1 or RAD51 SNPs. Combined XPD+RAD51 SNPs were highly associated with the disease, p≤0.005 [OR=3.40 (1.32-9.20)]. A Chi-Square significant association between XPD/Lys751Gln and Gleason score was also observed (OR=9.31; IC95%=1.19–428.0; p=0.022). Epidemiological inquiries revealed that exposure to pesticides significantly impacted the risk for prostate cancer in this population. DNA repair dysfunctions seem to prevail among workers exposed to chemical byproducts to cancer in this specific tissue. Non-invasive genotyping SNPs may help assessment of prostate cancer risk in environmentally exposed populations. |
| first_indexed | 2024-12-11T19:42:40Z |
| format | Article |
| id | doaj.art-2dddb01f54e84fbbb3ca26664902daf5 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-11T19:42:40Z |
| publishDate | 2017-11-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-2dddb01f54e84fbbb3ca26664902daf52022-12-22T00:52:59ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852017-11-01010.1590/1678-4685-gmb-2017-0039S1415-47572017005027105Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, BrazilAna Sheila CyprianoGilda AlvesAntonio Augusto OrnellasJosé ScheinkmanRenata AlmeidaLuciano ScherrerClaudia LageAbstract Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with confirmed prostate cancer and 200 unaffected men, from Rio de Janeiro, Brazil. XPD/Lys751Gln (rs13181), APEX1/Asp148Glu (rs1130409), and RAD51/G135C (rs1801320) SNPs were analyzed by PCR-RFLP. Allelic and genotypic frequencies were calculated and compared by Chi-Square test. The association between SNPs and clinical/epidemiological data was considered significant by Odds Ratio analysis, with IC95% and a p-value≤0.05. Only the XPD/Lys751Gln SNP significantly increased susceptibility to disease in southeastern Brazilian men, with p≤0.001 [OR=2.36 (1.46-3.84)], with no association with APEX1 or RAD51 SNPs. Combined XPD+RAD51 SNPs were highly associated with the disease, p≤0.005 [OR=3.40 (1.32-9.20)]. A Chi-Square significant association between XPD/Lys751Gln and Gleason score was also observed (OR=9.31; IC95%=1.19–428.0; p=0.022). Epidemiological inquiries revealed that exposure to pesticides significantly impacted the risk for prostate cancer in this population. DNA repair dysfunctions seem to prevail among workers exposed to chemical byproducts to cancer in this specific tissue. Non-invasive genotyping SNPs may help assessment of prostate cancer risk in environmentally exposed populations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017005027105&lng=en&tlng=enProstate cancersingle nucleotide polymorphismXPDDNA repairgene-environment interaction |
| spellingShingle | Ana Sheila Cypriano Gilda Alves Antonio Augusto Ornellas José Scheinkman Renata Almeida Luciano Scherrer Claudia Lage Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil Genetics and Molecular Biology Prostate cancer single nucleotide polymorphism XPD DNA repair gene-environment interaction |
| title | Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil |
| title_full | Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil |
| title_fullStr | Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil |
| title_full_unstemmed | Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil |
| title_short | Relationship between XPD, RAD51, and APEX1 DNA repair genotypes and prostate cancer risk in the male population of Rio de Janeiro, Brazil |
| title_sort | relationship between xpd rad51 and apex1 dna repair genotypes and prostate cancer risk in the male population of rio de janeiro brazil |
| topic | Prostate cancer single nucleotide polymorphism XPD DNA repair gene-environment interaction |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017005027105&lng=en&tlng=en |
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