Multiplexed evaluation of mouse wound tissue using oligonucleotide barcoding with single-cell RNA sequencing

Multiplexed evaluation of mouse wound tissue using oligonucleotide barcoding with single-cell RNA sequencing

Summary: Despite its rapidly increased availability for the study of complex tissue, single-cell RNA sequencing remains prohibitively expensive for large studies. Here, we present a protocol using oligonucleotide barcoding for the tagging and pooling of multiple samples from healing wounds, which ar...

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Bibliographic Details
Main Authors: Michael Januszyk, Michelle Griffin, Shamik Mascharak, Heather E. Talbott, Kellen Chen, Dominic Henn, Amanda F. Spielman, Jennifer B.L. Parker, Norah E. Liang, Asha Cotterell, Nicholas Guardino, Deshka S. Foster, Dhananjay Wagh, John Coller, Geoffrey C. Gurtner, Derrick C. Wan, Michael T. Longaker
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Single Cell
RNAseq
Model Organisms
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008267

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