Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans
Studies have shown evidence of production of nitric oxide (NO) in adipose tissue, as well as inhibition of lipolysis by NO. We have analyzed nitric oxide synthase (NOS) expression in subcutaneous adipose tissue from 13 nonobese and 18 obese male subjects. Using a competitive reverse transcription po...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2000-08-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520334325 |
| _version_ | 1818602538813882368 |
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| author | Montserrat Elizalde Mikael Rydén Vanessa van Harmelen Peter Eneroth Hans Gyllenhammar Cecilia Holm Stig Ramel Anders Ölund Peter Arner Kurt Andersson |
| author_facet | Montserrat Elizalde Mikael Rydén Vanessa van Harmelen Peter Eneroth Hans Gyllenhammar Cecilia Holm Stig Ramel Anders Ölund Peter Arner Kurt Andersson |
| author_sort | Montserrat Elizalde |
| collection | DOAJ |
| description | Studies have shown evidence of production of nitric oxide (NO) in adipose tissue, as well as inhibition of lipolysis by NO. We have analyzed nitric oxide synthase (NOS) expression in subcutaneous adipose tissue from 13 nonobese and 18 obese male subjects. Using a competitive reverse transcription polymerase chain reaction method, endothelial (eNOS) and inducible (iNOS), but not neuronal (nNOS), nitric oxide synthase mRNA expression was detected in isolated fat cells and pieces of adipose tissue. Tissue mRNA levels for eNOS were 3,814 ± 825 and 5,956 ± 476 amol/mg RNA (P = 0.043), and for iNOS 306 ± 38 and 332 ± 48 amol/mg RNA, for nonobese and obese individuals, respectively. Western blotting revealed similar eNOS protein levels in isolated fat cells and adipose tissue pieces. Protein levels for eNOS in nonobese and obese individuals, respectively, were (in optical density [OD] units per mm2 per 100 μg of total protein) 0.11 ± 0.08 and 2.80 ± 1.30 (P = 0.043). iNOS protein was detectable, but not measurable, at low levels in a subset of obese patients (3 of 10). iNOS protein levels could not be detected in nonobese individuals. Hormone-sensitive lipase (HSL), the key regulating enzyme in lipolysis, is reduced in obesity. The expression of HSL protein in subcutaneous adipose tissue was studied in the same subset of patients; in agreement with previous results, HSL levels were reduced in obese subjects: 4.64 ± 1.10 and 1.27 ± 0.35 (P = 0.012) in nonobese and obese subjects, respectively. In conclusion, this study shows that eNOS and iNOS, but not nNOS, are present in human subcutaneous adipose tissue. Gene expression and protein levels of eNOS are increased, whereas HSL protein levels are decreased in obesity. It is speculated that increased NO production, preferably by eNOS, and decreased HSL levels may cause decreased subcutaneous adipose tissue lipolysis in obesity. —Elizalde, M., M. Rydén, V. van Harmelen, P. Eneroth, H. Gyllenhammar, C. Holm, S. Ramel, A. Ölund, P. Arner, and K. Andersson. Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans. J. Lipid Res. 2000. 41: 1244–1251. |
| first_indexed | 2024-12-16T13:08:52Z |
| format | Article |
| id | doaj.art-2de87170fe5a4954b5427dfa37c73b21 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T13:08:52Z |
| publishDate | 2000-08-01 |
| publisher | Elsevier |
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| series | Journal of Lipid Research |
| spelling | doaj.art-2de87170fe5a4954b5427dfa37c73b212022-12-21T22:30:40ZengElsevierJournal of Lipid Research0022-22752000-08-0141812441251Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humansMontserrat Elizalde0Mikael Rydén1Vanessa van Harmelen2Peter Eneroth3Hans Gyllenhammar4Cecilia Holm5Stig Ramel6Anders Ölund7Peter Arner8Kurt Andersson9Center for Metabolism and Endocrinology, Huddinge Hospital, 141 86 Huddinge, SwedenCenter for Metabolism and Endocrinology, Huddinge Hospital, 141 86 Huddinge, SwedenCenter for Metabolism and Endocrinology, Huddinge Hospital, 141 86 Huddinge, SwedenUnit for Applied Biochemistry, Huddinge Hospital, 141 86 Huddinge, SwedenDepartment of Hematology, Huddinge Hospital, 141 86 Huddinge, SwedenDepartment of Cell and Molecular Biology, Lund University, 22185 Lund, SwedenDepartment of Surgery, Huddinge Hospital, 141 86 Huddinge, SwedenDepartment of Gynecology, Huddinge Hospital, 141 86 Huddinge, SwedenCenter for Metabolism and Endocrinology, Huddinge Hospital, 141 86 Huddinge, SwedenCenter for Metabolism and Endocrinology, Huddinge Hospital, 141 86 Huddinge, Sweden; To whom correspondence should be addressed.Studies have shown evidence of production of nitric oxide (NO) in adipose tissue, as well as inhibition of lipolysis by NO. We have analyzed nitric oxide synthase (NOS) expression in subcutaneous adipose tissue from 13 nonobese and 18 obese male subjects. Using a competitive reverse transcription polymerase chain reaction method, endothelial (eNOS) and inducible (iNOS), but not neuronal (nNOS), nitric oxide synthase mRNA expression was detected in isolated fat cells and pieces of adipose tissue. Tissue mRNA levels for eNOS were 3,814 ± 825 and 5,956 ± 476 amol/mg RNA (P = 0.043), and for iNOS 306 ± 38 and 332 ± 48 amol/mg RNA, for nonobese and obese individuals, respectively. Western blotting revealed similar eNOS protein levels in isolated fat cells and adipose tissue pieces. Protein levels for eNOS in nonobese and obese individuals, respectively, were (in optical density [OD] units per mm2 per 100 μg of total protein) 0.11 ± 0.08 and 2.80 ± 1.30 (P = 0.043). iNOS protein was detectable, but not measurable, at low levels in a subset of obese patients (3 of 10). iNOS protein levels could not be detected in nonobese individuals. Hormone-sensitive lipase (HSL), the key regulating enzyme in lipolysis, is reduced in obesity. The expression of HSL protein in subcutaneous adipose tissue was studied in the same subset of patients; in agreement with previous results, HSL levels were reduced in obese subjects: 4.64 ± 1.10 and 1.27 ± 0.35 (P = 0.012) in nonobese and obese subjects, respectively. In conclusion, this study shows that eNOS and iNOS, but not nNOS, are present in human subcutaneous adipose tissue. Gene expression and protein levels of eNOS are increased, whereas HSL protein levels are decreased in obesity. It is speculated that increased NO production, preferably by eNOS, and decreased HSL levels may cause decreased subcutaneous adipose tissue lipolysis in obesity. —Elizalde, M., M. Rydén, V. van Harmelen, P. Eneroth, H. Gyllenhammar, C. Holm, S. Ramel, A. Ölund, P. Arner, and K. Andersson. Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans. J. Lipid Res. 2000. 41: 1244–1251.http://www.sciencedirect.com/science/article/pii/S0022227520334325fat cellsNOShormone-sensitive lipasemessenger RNAprotein |
| spellingShingle | Montserrat Elizalde Mikael Rydén Vanessa van Harmelen Peter Eneroth Hans Gyllenhammar Cecilia Holm Stig Ramel Anders Ölund Peter Arner Kurt Andersson Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans Journal of Lipid Research fat cells NOS hormone-sensitive lipase messenger RNA protein |
| title | Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans |
| title_full | Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans |
| title_fullStr | Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans |
| title_full_unstemmed | Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans |
| title_short | Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans |
| title_sort | expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans |
| topic | fat cells NOS hormone-sensitive lipase messenger RNA protein |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520334325 |
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