Effect of magnesium chloride and guanidinum chloride on the extraction of components of extracellular matrix from chicken cartilage

Effect of magnesium chloride and guanidinum chloride on the extraction of components of extracellular matrix from chicken cartilage

In order to evaluate the effect of chaotropic agents on proteoglycan and non-collagenous proteins, chicken xiphoid cartilage was treated with guanidine-HCI and MgCl2 in different concentrations (1M to 5M), and different periods of time (12, 24, 48 and 72hr). The maximum yield of uronic acid was obta...

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Bibliographic Details
Main Authors: Kátia Denise Souza Arcanjo, Laurecir Gomes, Edson Rosa Pimentel
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 1994-03-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
proteoglycans
non-collagenous proteins
cartilage
extracelullar matrix
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000100016

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