Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level
Abstract The use of double‐barreled nanopipette (θ‐nanopipette) to electrically sample, manipulate, or detect biomaterials has recently seen strong growth in single‐cell studies, driven by the potential of the nanodevices and applications that they may enable. Considering the pivotal roles of Na/K r...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-10-01
|
| Series: | Exploration |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/EXP.20220025 |
| _version_ | 1811250337763295232 |
|---|---|
| author | Xiao‐Mei Shi Fang‐Qing Liu Bing Wang Si‐Yuan Yu Yi‐Tong Xu Wei‐Wei Zhao Dechen Jiang Hong‐Yuan Chen Jing‐Juan Xu |
| author_facet | Xiao‐Mei Shi Fang‐Qing Liu Bing Wang Si‐Yuan Yu Yi‐Tong Xu Wei‐Wei Zhao Dechen Jiang Hong‐Yuan Chen Jing‐Juan Xu |
| author_sort | Xiao‐Mei Shi |
| collection | DOAJ |
| description | Abstract The use of double‐barreled nanopipette (θ‐nanopipette) to electrically sample, manipulate, or detect biomaterials has recently seen strong growth in single‐cell studies, driven by the potential of the nanodevices and applications that they may enable. Considering the pivotal roles of Na/K ratio (RNa/K) at cellular level, herein we describe an engineered θ‐nanopipette for measuring single‐cell RNa/K. The two independently addressable nanopores, located within one nanotip, allow respective customization of functional nucleic acids but simultaneous deciphering of Na and K levels inside a single cell of a non‐Faradic manner. Two ionic current rectification signals, corresponding to the Na‐ and K‐specific smart DNA responses, could be easily used to derive the RNa/K. The applicability of this nanotool is validated by practical probing intracellular RNa/K during the drug‐induced primary stage of apoptotic volume decrease. Especially, the RNa/K has been shown by our nanotool to be different in cell lines with different metastatic potential. This work is expected to contribute to futuristic study of single‐cell RNa/K in various physiological and pathological processes. |
| first_indexed | 2024-04-12T16:03:08Z |
| format | Article |
| id | doaj.art-2dea6f948f1e4a50baae850062cc2382 |
| institution | Directory Open Access Journal |
| issn | 2766-8509 2766-2098 |
| language | English |
| last_indexed | 2024-04-12T16:03:08Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Exploration |
| spelling | doaj.art-2dea6f948f1e4a50baae850062cc23822022-12-22T03:26:09ZengWileyExploration2766-85092766-20982022-10-0125n/an/a10.1002/EXP.20220025Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell levelXiao‐Mei Shi0Fang‐Qing Liu1Bing Wang2Si‐Yuan Yu3Yi‐Tong Xu4Wei‐Wei Zhao5Dechen Jiang6Hong‐Yuan Chen7Jing‐Juan Xu8State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaState Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing P. R. ChinaAbstract The use of double‐barreled nanopipette (θ‐nanopipette) to electrically sample, manipulate, or detect biomaterials has recently seen strong growth in single‐cell studies, driven by the potential of the nanodevices and applications that they may enable. Considering the pivotal roles of Na/K ratio (RNa/K) at cellular level, herein we describe an engineered θ‐nanopipette for measuring single‐cell RNa/K. The two independently addressable nanopores, located within one nanotip, allow respective customization of functional nucleic acids but simultaneous deciphering of Na and K levels inside a single cell of a non‐Faradic manner. Two ionic current rectification signals, corresponding to the Na‐ and K‐specific smart DNA responses, could be easily used to derive the RNa/K. The applicability of this nanotool is validated by practical probing intracellular RNa/K during the drug‐induced primary stage of apoptotic volume decrease. Especially, the RNa/K has been shown by our nanotool to be different in cell lines with different metastatic potential. This work is expected to contribute to futuristic study of single‐cell RNa/K in various physiological and pathological processes.https://doi.org/10.1002/EXP.20220025θ‐nanopipetteionic current rectificationnucleic acidsingle cellsodium to potassium ratio |
| spellingShingle | Xiao‐Mei Shi Fang‐Qing Liu Bing Wang Si‐Yuan Yu Yi‐Tong Xu Wei‐Wei Zhao Dechen Jiang Hong‐Yuan Chen Jing‐Juan Xu Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level Exploration θ‐nanopipette ionic current rectification nucleic acid single cell sodium to potassium ratio |
| title | Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level |
| title_full | Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level |
| title_fullStr | Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level |
| title_full_unstemmed | Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level |
| title_short | Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level |
| title_sort | functional nucleic acid engineered double barreled nanopores for measuring sodium to potassium ratio at single cell level |
| topic | θ‐nanopipette ionic current rectification nucleic acid single cell sodium to potassium ratio |
| url | https://doi.org/10.1002/EXP.20220025 |
| work_keys_str_mv | AT xiaomeishi functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT fangqingliu functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT bingwang functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT siyuanyu functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT yitongxu functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT weiweizhao functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT dechenjiang functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT hongyuanchen functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel AT jingjuanxu functionalnucleicacidengineereddoublebarrelednanoporesformeasuringsodiumtopotassiumratioatsinglecelllevel |