Factors influencing vitamin B6 status in domestic cats: age, disease, and body condition score
Abstract Limited studies are available on vitamin B6 status in domestic cats. To this end, we evaluated glutamate–oxaloacetate transaminase (GOT) activity in hemolysates with and without pyridoxal 5′-phosphate addition in two feline populations: a cohort of 60 healthy, domestic (sexually intact and...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52367-y |
| _version_ | 1797276596413923328 |
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| author | Vy Chu Andrea J. Fascetti Jennifer A. Larsen Maria Montano Cecilia Giulivi |
| author_facet | Vy Chu Andrea J. Fascetti Jennifer A. Larsen Maria Montano Cecilia Giulivi |
| author_sort | Vy Chu |
| collection | DOAJ |
| description | Abstract Limited studies are available on vitamin B6 status in domestic cats. To this end, we evaluated glutamate–oxaloacetate transaminase (GOT) activity in hemolysates with and without pyridoxal 5′-phosphate addition in two feline populations: a cohort of 60 healthy, domestic (sexually intact and specific pathogen-free) cats maintained under strictly controlled conditions with appropriate diets housed at the Feline Nutrition and Pet Care Center, and a cohort of 57 cats randomly selected between December 2022 to January 2023 that visited the Veterinary Medicine Teaching Hospital to seek care under different circumstances. The GOT activity expressed as the ratio with and without pyridoxal 5′-phosphate addition (primary activation ratio; PAR) decreased significantly with age in the healthy cohort. The PAR values normalized to age established a cut-off for vitamin B6 deficiency in both cohorts, identifying 17 of 101 animals as vitamin B6 deficient. Using machine learning, a partition-based model (decision tree) was built to identify the most important factors that predicted vitamin B6 deficiency while using the resulting tree to make predictions for new observations. This analysis, performed with all 101 cats, revealed that the diagnosis of an infectious, chronic or acute condition (0.55) was the main contributor, followed by age (0.26), and body condition score (optimal-overweight; 0.19). Thus, our study supports that vitamin B6 supplementation may be indicated in junior to adult animals diagnosed with an infectious, chronic, or acute conditions or healthy cats with body weight ranging from optimal to overweight. In older cats, even if healthy, underweight to optimal cats appear to be at risk of vitamin B6 deficiency. |
| first_indexed | 2024-03-07T15:30:28Z |
| format | Article |
| id | doaj.art-2ded62cd7da5442fa8614675d8a2e4ad |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:30:28Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-2ded62cd7da5442fa8614675d8a2e4ad2024-03-05T16:25:36ZengNature PortfolioScientific Reports2045-23222024-01-0114111510.1038/s41598-024-52367-yFactors influencing vitamin B6 status in domestic cats: age, disease, and body condition scoreVy Chu0Andrea J. Fascetti1Jennifer A. Larsen2Maria Montano3Cecilia Giulivi4Department of Molecular Biosciences, University of California Davis, School of Veterinary MedicineDepartment of Molecular Biosciences, University of California Davis, School of Veterinary MedicineDepartment of Molecular Biosciences, University of California Davis, School of Veterinary MedicineDepartment of Molecular Biosciences, University of California Davis, School of Veterinary MedicineDepartment of Molecular Biosciences, University of California Davis, School of Veterinary MedicineAbstract Limited studies are available on vitamin B6 status in domestic cats. To this end, we evaluated glutamate–oxaloacetate transaminase (GOT) activity in hemolysates with and without pyridoxal 5′-phosphate addition in two feline populations: a cohort of 60 healthy, domestic (sexually intact and specific pathogen-free) cats maintained under strictly controlled conditions with appropriate diets housed at the Feline Nutrition and Pet Care Center, and a cohort of 57 cats randomly selected between December 2022 to January 2023 that visited the Veterinary Medicine Teaching Hospital to seek care under different circumstances. The GOT activity expressed as the ratio with and without pyridoxal 5′-phosphate addition (primary activation ratio; PAR) decreased significantly with age in the healthy cohort. The PAR values normalized to age established a cut-off for vitamin B6 deficiency in both cohorts, identifying 17 of 101 animals as vitamin B6 deficient. Using machine learning, a partition-based model (decision tree) was built to identify the most important factors that predicted vitamin B6 deficiency while using the resulting tree to make predictions for new observations. This analysis, performed with all 101 cats, revealed that the diagnosis of an infectious, chronic or acute condition (0.55) was the main contributor, followed by age (0.26), and body condition score (optimal-overweight; 0.19). Thus, our study supports that vitamin B6 supplementation may be indicated in junior to adult animals diagnosed with an infectious, chronic, or acute conditions or healthy cats with body weight ranging from optimal to overweight. In older cats, even if healthy, underweight to optimal cats appear to be at risk of vitamin B6 deficiency.https://doi.org/10.1038/s41598-024-52367-y |
| spellingShingle | Vy Chu Andrea J. Fascetti Jennifer A. Larsen Maria Montano Cecilia Giulivi Factors influencing vitamin B6 status in domestic cats: age, disease, and body condition score Scientific Reports |
| title | Factors influencing vitamin B6 status in domestic cats: age, disease, and body condition score |
| title_full | Factors influencing vitamin B6 status in domestic cats: age, disease, and body condition score |
| title_fullStr | Factors influencing vitamin B6 status in domestic cats: age, disease, and body condition score |
| title_full_unstemmed | Factors influencing vitamin B6 status in domestic cats: age, disease, and body condition score |
| title_short | Factors influencing vitamin B6 status in domestic cats: age, disease, and body condition score |
| title_sort | factors influencing vitamin b6 status in domestic cats age disease and body condition score |
| url | https://doi.org/10.1038/s41598-024-52367-y |
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