Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot Study
In this pilot study, we aim to determine differences in pathogenetic pathways between interstitial pneumonia with autoimmune features (IPAF), connective-tissue-disease-associated interstitial lung diseases (CTD-ILDs), and idiopathic interstitial pneumonias (IIPs). Forty participants were recruited:...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/15205 |
| _version_ | 1797462989264125952 |
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| author | Patrycja Rzepka-Wrona Szymon Skoczyński Adam Barczyk |
| author_facet | Patrycja Rzepka-Wrona Szymon Skoczyński Adam Barczyk |
| author_sort | Patrycja Rzepka-Wrona |
| collection | DOAJ |
| description | In this pilot study, we aim to determine differences in pathogenetic pathways between interstitial pneumonia with autoimmune features (IPAF), connective-tissue-disease-associated interstitial lung diseases (CTD-ILDs), and idiopathic interstitial pneumonias (IIPs). Forty participants were recruited: 9 with IPAF, 15 with CTD-ILDs, and 16 with IIPs. Concentration of transforming growth factor beta (TGF-β1), surfactant proteins A and D (SP-A, SP-D), interleukin 8 (IL-8), and chemokine 1 (CXCL1) were assessed with ELISA assay in bronchoalveolar lavage (BAL) fluid. We revealed that IL-8 and TGF-β1 concentrations were significantly lower in the IPAF group than in the CTD-ILD group (<i>p</i> = 0.008 and <i>p</i> = 0.019, respectively), but similar to the concentrations in the IIP group. There were significant correlations of IL-8 (rs = 0.46; <i>p</i> = 0.003) and CXCL1 (rs = 0.52; <i>p</i> = 0.001) and BAL total cell count (TCC). A multivariate regression model revealed that IL-8 (β = 0.32; <i>p</i> = 0.037) and CXCL1 (β = 0.45; <i>p</i> = 0.004) are significant predictors of BAL TCC. We revealed that IL-8 and TGF-β1 BAL concentrations vary in patients with different ILDs and found that IL-8 is a predictor of BAL TCC in IPAF. However, this needs to be confirmed in a multicenter cooperative study (ClinicalTrials.gov Identifier: NCT03870828). |
| first_indexed | 2024-03-09T17:44:18Z |
| format | Article |
| id | doaj.art-2def9c9e2dd442acb0257b4fbc395a7c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:44:18Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2def9c9e2dd442acb0257b4fbc395a7c2023-11-24T11:14:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123231520510.3390/ijms232315205Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot StudyPatrycja Rzepka-Wrona0Szymon Skoczyński1Adam Barczyk2Department of Pneumonology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Pneumonology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Pneumonology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-055 Katowice, PolandIn this pilot study, we aim to determine differences in pathogenetic pathways between interstitial pneumonia with autoimmune features (IPAF), connective-tissue-disease-associated interstitial lung diseases (CTD-ILDs), and idiopathic interstitial pneumonias (IIPs). Forty participants were recruited: 9 with IPAF, 15 with CTD-ILDs, and 16 with IIPs. Concentration of transforming growth factor beta (TGF-β1), surfactant proteins A and D (SP-A, SP-D), interleukin 8 (IL-8), and chemokine 1 (CXCL1) were assessed with ELISA assay in bronchoalveolar lavage (BAL) fluid. We revealed that IL-8 and TGF-β1 concentrations were significantly lower in the IPAF group than in the CTD-ILD group (<i>p</i> = 0.008 and <i>p</i> = 0.019, respectively), but similar to the concentrations in the IIP group. There were significant correlations of IL-8 (rs = 0.46; <i>p</i> = 0.003) and CXCL1 (rs = 0.52; <i>p</i> = 0.001) and BAL total cell count (TCC). A multivariate regression model revealed that IL-8 (β = 0.32; <i>p</i> = 0.037) and CXCL1 (β = 0.45; <i>p</i> = 0.004) are significant predictors of BAL TCC. We revealed that IL-8 and TGF-β1 BAL concentrations vary in patients with different ILDs and found that IL-8 is a predictor of BAL TCC in IPAF. However, this needs to be confirmed in a multicenter cooperative study (ClinicalTrials.gov Identifier: NCT03870828).https://www.mdpi.com/1422-0067/23/23/15205interstitial pneumoniabronchoalveolar lavageinterstitial lung diseaseIPAFCTD-ILDsIIPs |
| spellingShingle | Patrycja Rzepka-Wrona Szymon Skoczyński Adam Barczyk Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot Study International Journal of Molecular Sciences interstitial pneumonia bronchoalveolar lavage interstitial lung disease IPAF CTD-ILDs IIPs |
| title | Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot Study |
| title_full | Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot Study |
| title_fullStr | Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot Study |
| title_full_unstemmed | Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot Study |
| title_short | Are There Differences in Inflammatory and Fibrotic Pathways between IPAF, CTD-ILDs, and IIPs? A Single-Center Pilot Study |
| title_sort | are there differences in inflammatory and fibrotic pathways between ipaf ctd ilds and iips a single center pilot study |
| topic | interstitial pneumonia bronchoalveolar lavage interstitial lung disease IPAF CTD-ILDs IIPs |
| url | https://www.mdpi.com/1422-0067/23/23/15205 |
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