Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay
Sensitivity of the molecular diagnostic tests of extrapulmonary tuberculosis largely depends upon the efficiency of DNA extraction methods. The objective of our study was to compare three methods of extracting DNA of Mycobacterium tuberculosis for testing by polymerase chain reaction. All three meth...
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| Format: | Article |
| Language: | English |
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Ibrahim Medical College
2012-01-01
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| Series: | IMC Journal of Medical Science |
| Subjects: | |
| Online Access: | http://www.imcjms.com/registration/journal_full_text/49 |
| _version_ | 1811262225998938112 |
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| author | Khandaker Shadia Shaheda Anwar Sayera Banu Ahmed Abu Saleh Md. Ruhul Amin Miah |
| author_facet | Khandaker Shadia Shaheda Anwar Sayera Banu Ahmed Abu Saleh Md. Ruhul Amin Miah |
| author_sort | Khandaker Shadia |
| collection | DOAJ |
| description | Sensitivity of the molecular diagnostic tests of extrapulmonary tuberculosis largely depends upon the efficiency of DNA extraction methods. The objective of our study was to compare three methods of extracting DNA of Mycobacterium tuberculosis for testing by polymerase chain reaction. All three methods; heating, heating with sonication and addition of lysis buffer with heating and sonication were implicated on 20 extrapulmonary samples. PCR positivity was 2 (10%), 4 (20%) and 7 (35%) in the samples extracted by heating, heat+sonication and heat+sonication+lysis buffer method respectively. Of the extraction methods evaluated, maximum PCR positive results were achieved by combined heat, sonication and lysis buffer method which can be applied in routine clinical practice.
Ibrahim Med. Coll. J. 2012; 6(1): 9-11 |
| first_indexed | 2024-04-12T19:21:20Z |
| format | Article |
| id | doaj.art-2defcd738c644679965eddbea19ce926 |
| institution | Directory Open Access Journal |
| issn | 2519-1721 2519-1586 |
| language | English |
| last_indexed | 2024-04-12T19:21:20Z |
| publishDate | 2012-01-01 |
| publisher | Ibrahim Medical College |
| record_format | Article |
| series | IMC Journal of Medical Science |
| spelling | doaj.art-2defcd738c644679965eddbea19ce9262022-12-22T03:19:35ZengIbrahim Medical CollegeIMC Journal of Medical Science2519-17212519-15862012-01-0161911Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assayKhandaker Shadia0Shaheda Anwar1Sayera Banu2Ahmed Abu Saleh3Md. Ruhul Amin Miah4Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, BangladeshDepartment of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, BangladeshLaboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDRB), Mohakhali, Dhaka, BangladeshDepartment of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, BangladeshDepartment of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka, BangladeshSensitivity of the molecular diagnostic tests of extrapulmonary tuberculosis largely depends upon the efficiency of DNA extraction methods. The objective of our study was to compare three methods of extracting DNA of Mycobacterium tuberculosis for testing by polymerase chain reaction. All three methods; heating, heating with sonication and addition of lysis buffer with heating and sonication were implicated on 20 extrapulmonary samples. PCR positivity was 2 (10%), 4 (20%) and 7 (35%) in the samples extracted by heating, heat+sonication and heat+sonication+lysis buffer method respectively. Of the extraction methods evaluated, maximum PCR positive results were achieved by combined heat, sonication and lysis buffer method which can be applied in routine clinical practice. Ibrahim Med. Coll. J. 2012; 6(1): 9-11http://www.imcjms.com/registration/journal_full_text/49TuberculosisextrapulmonaryDNAextraction |
| spellingShingle | Khandaker Shadia Shaheda Anwar Sayera Banu Ahmed Abu Saleh Md. Ruhul Amin Miah Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay IMC Journal of Medical Science Tuberculosis extrapulmonary DNA extraction |
| title | Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay |
| title_full | Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay |
| title_fullStr | Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay |
| title_full_unstemmed | Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay |
| title_short | Comparison of three mycobacterial DNA extraction methods from extrapulmonary samples for PCR assay |
| title_sort | comparison of three mycobacterial dna extraction methods from extrapulmonary samples for pcr assay |
| topic | Tuberculosis extrapulmonary DNA extraction |
| url | http://www.imcjms.com/registration/journal_full_text/49 |
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