Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules
The purpose of this paper was to evaluate the liquid culture media for the production of Metarhizium anisopliae (IBCB 425) and Beauveria bassiana (IBCB 66), as well as the tolerance of these seedlings to the ultraviolet action and to the temperature. Twelve treatments composed of combinations betwee...
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| Format: | Article |
| Language: | English |
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Instituto Biológico
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| Series: | Arquivos do Instituto Biológico |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572014000400342&lng=en&tlng=en |
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| author | Emma Luize Ottati-de-Lima Antonio Batista Filho José Eduardo Marcondes de Almeida Mariana Hollanda Gassen Inajá Marchizeli Wenzel Aline Maria Belasco de Almeida Luciano Olmos Zapellini |
| author_facet | Emma Luize Ottati-de-Lima Antonio Batista Filho José Eduardo Marcondes de Almeida Mariana Hollanda Gassen Inajá Marchizeli Wenzel Aline Maria Belasco de Almeida Luciano Olmos Zapellini |
| author_sort | Emma Luize Ottati-de-Lima |
| collection | DOAJ |
| description | The purpose of this paper was to evaluate the liquid culture media for the production of Metarhizium anisopliae (IBCB 425) and Beauveria bassiana (IBCB 66), as well as the tolerance of these seedlings to the ultraviolet action and to the temperature. Twelve treatments composed of combinations between carbon and nitrogen concentrations were assessed. In order to determine the effect of ultraviolet radiation, plates with blastos-pores were exposed to it for 25 and 50 seconds. To determine the temperature efect, blastospores from culture media were exposed to 20, 25, 30 and 35°. For the virulence experiments, caterpillars of Diatraea saccharalis were sprayed with 2 mL of fungal suspension with the aid of a Potter tower. Te best media for M. anisopliae are 16.00 g (carbon) + 7.00 g (nitrogen) and 14.40 g (carbon) + 7.00 g (nitrogen), whereas for B. bassiana: 20.00 g (carbon) + 6.30 g (nitrogen) and 20.00 g (carbon) + 7.00 g (nitrogen). Te longer the exposure to ultraviolet radiation, the smaller the number of colonies. At 35°, there is a significant decrease in the formation of colonies. Te produced seedlings of fungi are pathogenic to D. saccharalis. |
| first_indexed | 2024-12-20T04:30:03Z |
| format | Article |
| id | doaj.art-2deff27c5f20426db3eadcabaf86090f |
| institution | Directory Open Access Journal |
| issn | 1808-1657 |
| language | English |
| last_indexed | 2024-12-20T04:30:03Z |
| publisher | Instituto Biológico |
| record_format | Article |
| series | Arquivos do Instituto Biológico |
| spelling | doaj.art-2deff27c5f20426db3eadcabaf86090f2022-12-21T19:53:25ZengInstituto BiológicoArquivos do Instituto Biológico1808-165781434235010.1590/1808-1657001352012S1808-16572014000400342Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagulesEmma Luize Ottati-de-LimaAntonio Batista FilhoJosé Eduardo Marcondes de AlmeidaMariana Hollanda GassenInajá Marchizeli WenzelAline Maria Belasco de AlmeidaLuciano Olmos ZapelliniThe purpose of this paper was to evaluate the liquid culture media for the production of Metarhizium anisopliae (IBCB 425) and Beauveria bassiana (IBCB 66), as well as the tolerance of these seedlings to the ultraviolet action and to the temperature. Twelve treatments composed of combinations between carbon and nitrogen concentrations were assessed. In order to determine the effect of ultraviolet radiation, plates with blastos-pores were exposed to it for 25 and 50 seconds. To determine the temperature efect, blastospores from culture media were exposed to 20, 25, 30 and 35°. For the virulence experiments, caterpillars of Diatraea saccharalis were sprayed with 2 mL of fungal suspension with the aid of a Potter tower. Te best media for M. anisopliae are 16.00 g (carbon) + 7.00 g (nitrogen) and 14.40 g (carbon) + 7.00 g (nitrogen), whereas for B. bassiana: 20.00 g (carbon) + 6.30 g (nitrogen) and 20.00 g (carbon) + 7.00 g (nitrogen). Te longer the exposure to ultraviolet radiation, the smaller the number of colonies. At 35°, there is a significant decrease in the formation of colonies. Te produced seedlings of fungi are pathogenic to D. saccharalis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572014000400342&lng=en&tlng=enBeauveria bassianaMetarhizium anisopliaefungi productionmicrobial controlsugarcane borer |
| spellingShingle | Emma Luize Ottati-de-Lima Antonio Batista Filho José Eduardo Marcondes de Almeida Mariana Hollanda Gassen Inajá Marchizeli Wenzel Aline Maria Belasco de Almeida Luciano Olmos Zapellini Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules Arquivos do Instituto Biológico Beauveria bassiana Metarhizium anisopliae fungi production microbial control sugarcane borer |
| title | Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules |
| title_full | Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules |
| title_fullStr | Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules |
| title_full_unstemmed | Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules |
| title_short | Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules |
| title_sort | liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules |
| topic | Beauveria bassiana Metarhizium anisopliae fungi production microbial control sugarcane borer |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572014000400342&lng=en&tlng=en |
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