Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats
Abstract Background Libidibia ferrea (L. ferrea) has been used in folk medicine to treat several conditions and to prevent cancer. This study performed a chromatographic analysis of the crude aqueous extract of Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz (LfAE) leaves and evaluated its in vivo an...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-02-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-019-2454-3 |
| _version_ | 1811214980346806272 |
|---|---|
| author | Tamires Rocha Falcão Cássio Alexandre Oliveira Rodrigues Aurigena Antunes de Araújo Caroline Addison Carvalho Xavier de Medeiros Luiz Alberto Lira Soares Magda Rhayanny Assunção Ferreira Roseane Carvalho Vasconcelos Raimundo Fernandes de Araújo Júnior Maria Luiza Diniz de Sousa Lopes Gerlane Coelho Bernardo Guerra |
| author_facet | Tamires Rocha Falcão Cássio Alexandre Oliveira Rodrigues Aurigena Antunes de Araújo Caroline Addison Carvalho Xavier de Medeiros Luiz Alberto Lira Soares Magda Rhayanny Assunção Ferreira Roseane Carvalho Vasconcelos Raimundo Fernandes de Araújo Júnior Maria Luiza Diniz de Sousa Lopes Gerlane Coelho Bernardo Guerra |
| author_sort | Tamires Rocha Falcão |
| collection | DOAJ |
| description | Abstract Background Libidibia ferrea (L. ferrea) has been used in folk medicine to treat several conditions and to prevent cancer. This study performed a chromatographic analysis of the crude aqueous extract of Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz (LfAE) leaves and evaluated its in vivo antioxidant and anti-inflammatory potential. Methods Polyphenols present in LfAE were characterized by high performance liquid chromatography (HPLC). Anti-inflammatory activity was studied in an experimental model of zymosan-induced intra-articular inflammation, conducted in Wistar rats treated with LfAE at the doses of 100, 200 and 300 mg/kg by gavage. Synovial fluid was collected for global leukocyte count, for spectrocopical UV/VIS analysis of myeloperoxidase (MPO) activity, total glutathione and malondialdehyde (MDA), and for quantification of inflammatory cytokines IL1-β and TNF-α by enzyme-linked immunosorbent assay. Synovial membrane was collected for histological analysis. The level of statistical significance was p < 0.05. Results HPLC detected concentrations of 1.56 (0.77) %m/m for ellagic acid and 1.20 (1.38) %m/m for gallic acid in LfAE leaves. Treatment with LfAE at all doses significantly decreased the leukocyte influx into the synovial fluid (p < 0.001) and myeloperoxidase activity (p < 0.001), an important marker of neutrophils. LfAE at doses of 100 (p < 0.05), 200 and 300 mg/kg (p < 0.001) also reduced the levels of MDA. LfAE at doses of 200 and 300 mg/kg significantly decreased the levels of IL-1β (p < 0.05) and TNF-α (p < 0.001). All doses of LfAE resulted in increased levels of total glutathione (p < 0.001). Histopathological findings confirmed a reduction of the inflammatory infiltrate in the rats treated with LfAE at a dose of 200 mg/kg (p < 0.05). Conclusion LfAE has an important anti-oxidant and anti-inflammatory effect on intra-articular inflammation. |
| first_indexed | 2024-04-12T06:14:42Z |
| format | Article |
| id | doaj.art-2df2e922756a40f9954a57eece13f3d3 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-04-12T06:14:42Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-2df2e922756a40f9954a57eece13f3d32022-12-22T03:44:32ZengBMCBMC Complementary and Alternative Medicine1472-68822019-02-0119111010.1186/s12906-019-2454-3Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in ratsTamires Rocha Falcão0Cássio Alexandre Oliveira Rodrigues1Aurigena Antunes de Araújo2Caroline Addison Carvalho Xavier de Medeiros3Luiz Alberto Lira Soares4Magda Rhayanny Assunção Ferreira5Roseane Carvalho Vasconcelos6Raimundo Fernandes de Araújo Júnior7Maria Luiza Diniz de Sousa Lopes8Gerlane Coelho Bernardo Guerra9Postgraduate program in Pharmaceutical Science / Department of Biophysics and Pharmacology, UFRNDepartment of Biophysics and Pharmacology, UFRNPostgraduate program in Oral Science / Postgraduate program in Pharmaceutical Science/ Department of Biophysics and Pharmacology, Centro de Biociências/UFRNPostgraduate in Biological Science / Postgraduate program in RENORBIO /Department of Biophysics and Pharmacology, UFRNLaboratory of Pharmacognosy / Postgraduate program in Therapeutic Innovation/ Department of Pharmaceutical Sciences, UFPELaboratory of Pharmacognosy / Postgraduate program in Therapeutic Innovation/ Department of Pharmaceutical Sciences, UFPEPostdoctoral in the Postgraduate program in Public Health. Department of Dentistry, UFRNPost graduation program in Functional and Structural Biology/Post graduation program Health Science/Department of Morphology, UFRNPostdoctoral in the Postgraduate program in Public Health. Department of Dentistry, UFRNPost Graduation in Biological Science/Post graduation program in Pharmaceutical Science/Department of Biophysics and Pharmacology, UFRNAbstract Background Libidibia ferrea (L. ferrea) has been used in folk medicine to treat several conditions and to prevent cancer. This study performed a chromatographic analysis of the crude aqueous extract of Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz (LfAE) leaves and evaluated its in vivo antioxidant and anti-inflammatory potential. Methods Polyphenols present in LfAE were characterized by high performance liquid chromatography (HPLC). Anti-inflammatory activity was studied in an experimental model of zymosan-induced intra-articular inflammation, conducted in Wistar rats treated with LfAE at the doses of 100, 200 and 300 mg/kg by gavage. Synovial fluid was collected for global leukocyte count, for spectrocopical UV/VIS analysis of myeloperoxidase (MPO) activity, total glutathione and malondialdehyde (MDA), and for quantification of inflammatory cytokines IL1-β and TNF-α by enzyme-linked immunosorbent assay. Synovial membrane was collected for histological analysis. The level of statistical significance was p < 0.05. Results HPLC detected concentrations of 1.56 (0.77) %m/m for ellagic acid and 1.20 (1.38) %m/m for gallic acid in LfAE leaves. Treatment with LfAE at all doses significantly decreased the leukocyte influx into the synovial fluid (p < 0.001) and myeloperoxidase activity (p < 0.001), an important marker of neutrophils. LfAE at doses of 100 (p < 0.05), 200 and 300 mg/kg (p < 0.001) also reduced the levels of MDA. LfAE at doses of 200 and 300 mg/kg significantly decreased the levels of IL-1β (p < 0.05) and TNF-α (p < 0.001). All doses of LfAE resulted in increased levels of total glutathione (p < 0.001). Histopathological findings confirmed a reduction of the inflammatory infiltrate in the rats treated with LfAE at a dose of 200 mg/kg (p < 0.05). Conclusion LfAE has an important anti-oxidant and anti-inflammatory effect on intra-articular inflammation.http://link.springer.com/article/10.1186/s12906-019-2454-3Libidibia ferreaIntra-articularInflammationOxidative stress |
| spellingShingle | Tamires Rocha Falcão Cássio Alexandre Oliveira Rodrigues Aurigena Antunes de Araújo Caroline Addison Carvalho Xavier de Medeiros Luiz Alberto Lira Soares Magda Rhayanny Assunção Ferreira Roseane Carvalho Vasconcelos Raimundo Fernandes de Araújo Júnior Maria Luiza Diniz de Sousa Lopes Gerlane Coelho Bernardo Guerra Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats BMC Complementary and Alternative Medicine Libidibia ferrea Intra-articular Inflammation Oxidative stress |
| title | Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats |
| title_full | Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats |
| title_fullStr | Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats |
| title_full_unstemmed | Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats |
| title_short | Crude extract from Libidibia ferrea (Mart. ex. Tul.) L.P. Queiroz leaves decreased intra articular inflammation induced by zymosan in rats |
| title_sort | crude extract from libidibia ferrea mart ex tul l p queiroz leaves decreased intra articular inflammation induced by zymosan in rats |
| topic | Libidibia ferrea Intra-articular Inflammation Oxidative stress |
| url | http://link.springer.com/article/10.1186/s12906-019-2454-3 |
| work_keys_str_mv | AT tamiresrochafalcao crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT cassioalexandreoliveirarodrigues crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT aurigenaantunesdearaujo crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT carolineaddisoncarvalhoxavierdemedeiros crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT luizalbertolirasoares crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT magdarhayannyassuncaoferreira crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT roseanecarvalhovasconcelos crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT raimundofernandesdearaujojunior crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT marialuizadinizdesousalopes crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats AT gerlanecoelhobernardoguerra crudeextractfromlibidibiaferreamartextullpqueirozleavesdecreasedintraarticularinflammationinducedbyzymosaninrats |