Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effect
In recent years, methane concentration has increased rapidly due to agricultural production activities, in which livestock production is a significant source of methane emissions. Methane is the second-largest carbon compound in the atmosphere and 25 times more radiatively effective than carbon diox...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
2023-06-01
|
| Series: | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
| Subjects: | |
| Online Access: | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/2351 |
| _version_ | 1797653538003746816 |
|---|---|
| author | Nguyễn Văn Minh Dương Nhật Linh Đinh Thị Mai Anh Nguyễn Hoài Linh Trần Kiến Đức |
| author_facet | Nguyễn Văn Minh Dương Nhật Linh Đinh Thị Mai Anh Nguyễn Hoài Linh Trần Kiến Đức |
| author_sort | Nguyễn Văn Minh |
| collection | DOAJ |
| description | In recent years, methane concentration has increased rapidly due to agricultural production activities, in which livestock production is a significant source of methane emissions. Methane is the second-largest carbon compound in the atmosphere and 25 times more radiatively effective than carbon dioxide which is the main reason for ozone depletion. In this study, strains of bacteria capable of oxidizing methane (Methane Oxidizing Bacteria - MOB) were isolated and screened. From 13 samples of mud soil, 32 samples of rice land, 04 samples of biogas wastewater, 03 samples of the rumen, and 04 samples of river water, we isolated 370 bacterial strains capable of reducing CH4 on dAMS medium with CH4 being the sole carbon source. Quantitative results show that 19 bacterial strains can reduce CH4 including two strains TB18 and DC11 isolated from rice soil and rumen have the highest ability to oxidize methane (32.6 ± 0.25%, 30.2 ± 0.14%). The results of this study are the premise for the application of MOB bacteria in order to produce microbial products so that CH4 emissions can be reduced in Vietnam’s agriculture and raising cattle. |
| first_indexed | 2024-03-11T16:46:01Z |
| format | Article |
| id | doaj.art-2df342e8632545f6a096e564d30958e7 |
| institution | Directory Open Access Journal |
| issn | 2734-9322 2734-9594 |
| language | English |
| last_indexed | 2024-03-11T16:46:01Z |
| publishDate | 2023-06-01 |
| publisher | HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
| spelling | doaj.art-2df342e8632545f6a096e564d30958e72023-10-22T15:33:56ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCETạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ2734-93222734-95942023-06-01182899810.46223/HCMCOUJS.tech.vi.18.2.2351.20231889Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effectNguyễn Văn Minh0Dương Nhật Linh1Đinh Thị Mai Anh2Nguyễn Hoài Linh3Trần Kiến Đức4Trường Đại học Mở Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrường Đại học Mở Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrường Đại học Mở Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrường Đại học Mở Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhTrường Đại học Khoa học Tự Nhiên, Thành phố Hồ Chí Minh Đại học Quốc Gia Thành phố Hồ Chí Minh, Thành phố Hồ Chí MinhIn recent years, methane concentration has increased rapidly due to agricultural production activities, in which livestock production is a significant source of methane emissions. Methane is the second-largest carbon compound in the atmosphere and 25 times more radiatively effective than carbon dioxide which is the main reason for ozone depletion. In this study, strains of bacteria capable of oxidizing methane (Methane Oxidizing Bacteria - MOB) were isolated and screened. From 13 samples of mud soil, 32 samples of rice land, 04 samples of biogas wastewater, 03 samples of the rumen, and 04 samples of river water, we isolated 370 bacterial strains capable of reducing CH4 on dAMS medium with CH4 being the sole carbon source. Quantitative results show that 19 bacterial strains can reduce CH4 including two strains TB18 and DC11 isolated from rice soil and rumen have the highest ability to oxidize methane (32.6 ± 0.25%, 30.2 ± 0.14%). The results of this study are the premise for the application of MOB bacteria in order to produce microbial products so that CH4 emissions can be reduced in Vietnam’s agriculture and raising cattle.https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/2351enzyme methane monooxygenasegiảm hiệu ứng nhà kínhmethanotrophsphân lậpvi khuẩn oxy hóa methane |
| spellingShingle | Nguyễn Văn Minh Dương Nhật Linh Đinh Thị Mai Anh Nguyễn Hoài Linh Trần Kiến Đức Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effect Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ enzyme methane monooxygenase giảm hiệu ứng nhà kính methanotrophs phân lập vi khuẩn oxy hóa methane |
| title | Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effect |
| title_full | Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effect |
| title_fullStr | Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effect |
| title_full_unstemmed | Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effect |
| title_short | Isolation and screening of bacteria capable of oxidizing methane - causing greenhouse effect |
| title_sort | isolation and screening of bacteria capable of oxidizing methane causing greenhouse effect |
| topic | enzyme methane monooxygenase giảm hiệu ứng nhà kính methanotrophs phân lập vi khuẩn oxy hóa methane |
| url | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/2351 |
| work_keys_str_mv | AT nguyenvanminh isolationandscreeningofbacteriacapableofoxidizingmethanecausinggreenhouseeffect AT duongnhatlinh isolationandscreeningofbacteriacapableofoxidizingmethanecausinggreenhouseeffect AT đinhthimaianh isolationandscreeningofbacteriacapableofoxidizingmethanecausinggreenhouseeffect AT nguyenhoailinh isolationandscreeningofbacteriacapableofoxidizingmethanecausinggreenhouseeffect AT trankienđuc isolationandscreeningofbacteriacapableofoxidizingmethanecausinggreenhouseeffect |