Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens
(1) Background: This study was aimed at determining the <i>in vitro</i> inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera <i>Alternaria</i>, <i>Colletotrichum</i>, <i>Fusarium&l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/11/2452 |
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| author | Soumia El boumlasy Federico La Spada Nunzio Tuccitto Giovanni Marletta Carlos Luz Mínguez Giuseppe Meca Ermes Ivan Rovetto Antonella Pane Abderrahmane Debdoubi Santa Olga Cacciola |
| author_facet | Soumia El boumlasy Federico La Spada Nunzio Tuccitto Giovanni Marletta Carlos Luz Mínguez Giuseppe Meca Ermes Ivan Rovetto Antonella Pane Abderrahmane Debdoubi Santa Olga Cacciola |
| author_sort | Soumia El boumlasy |
| collection | DOAJ |
| description | (1) Background: This study was aimed at determining the <i>in vitro</i> inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera <i>Alternaria</i>, <i>Colletotrichum</i>, <i>Fusarium</i>, <i>Penicillium</i>, <i>Plenodomus</i> and <i>Phytophthora</i>; the effectiveness of the substance with the highest <i>in vitro</i> activity in preventing citrus and apple fruit rot incited by <i>P. digitatum</i> and <i>P. expansum</i>, respectively, was also evaluated. (2) Methods: The four tested substances, water-extract, EtOAc-extract, MetOH-extract and nitric-extract, were analyzed by HPLC-ESI-MS-TOF; <i>in vitro</i> preliminary tests were carried out to determine the minimal inhibitory/fungicidal concentrations (MIC and MFC, respectively) of the raw dry powder, EtOAc-extract, MetOH-extract and nitric-extract for each pathogen. (3) Results: in the agar-diffusion-assay, nitric-extract showed an inhibitory effect on all pathogens, at all concentrations tested (100, 75, 50 and 25%); the maximum activity was on <i>Plenodomus tracheiphilus</i>, <i>C. gloeosporioides</i> and <i>Ph. nicotianae</i>; the diameters of inhibition halos were directly proportional to the extract concentration; values of MIC and MFC of this extract for all pathogens ranged from 2 to 3.5%; the highest concentrations (50 to 100%) tested <i>in vivo</i> were effective in preventing citrus and apple fruit molds. (4) Conclusions: This study contributes to the search for natural and ecofriendly substances for the control of pre- and post-harvest plant pathogens. |
| first_indexed | 2024-03-10T05:07:39Z |
| format | Article |
| id | doaj.art-2df40ca8f1bb40489b6b8242648ba61a |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T05:07:39Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-2df40ca8f1bb40489b6b8242648ba61a2023-11-23T01:05:59ZengMDPI AGPlants2223-77472021-11-011011245210.3390/plants10112452Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant PathogensSoumia El boumlasy0Federico La Spada1Nunzio Tuccitto2Giovanni Marletta3Carlos Luz Mínguez4Giuseppe Meca5Ermes Ivan Rovetto6Antonella Pane7Abderrahmane Debdoubi8Santa Olga Cacciola9Laboratory of Materials-Catalysis, Chemistry Department, Faculty of Science, University Abdelmalek Essaadi, Tetouan B.P. 2117, MoroccoDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyConsorzio per lo Sviluppo dei Sistemi a Grande Interfase, CSGI, Viale A. Doria 6, 95125 Catania, ItalyConsorzio per lo Sviluppo dei Sistemi a Grande Interfase, CSGI, Viale A. Doria 6, 95125 Catania, ItalyLaboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, SpainLaboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, SpainDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, ItalyLaboratory of Materials-Catalysis, Chemistry Department, Faculty of Science, University Abdelmalek Essaadi, Tetouan B.P. 2117, MoroccoDepartment of Agriculture, Food and Environment, University of Catania, 95123 Catania, Italy(1) Background: This study was aimed at determining the <i>in vitro</i> inhibitory effect of new natural substances obtained by minimal processing from shrimp wastes on fungi and oomycetes in the genera <i>Alternaria</i>, <i>Colletotrichum</i>, <i>Fusarium</i>, <i>Penicillium</i>, <i>Plenodomus</i> and <i>Phytophthora</i>; the effectiveness of the substance with the highest <i>in vitro</i> activity in preventing citrus and apple fruit rot incited by <i>P. digitatum</i> and <i>P. expansum</i>, respectively, was also evaluated. (2) Methods: The four tested substances, water-extract, EtOAc-extract, MetOH-extract and nitric-extract, were analyzed by HPLC-ESI-MS-TOF; <i>in vitro</i> preliminary tests were carried out to determine the minimal inhibitory/fungicidal concentrations (MIC and MFC, respectively) of the raw dry powder, EtOAc-extract, MetOH-extract and nitric-extract for each pathogen. (3) Results: in the agar-diffusion-assay, nitric-extract showed an inhibitory effect on all pathogens, at all concentrations tested (100, 75, 50 and 25%); the maximum activity was on <i>Plenodomus tracheiphilus</i>, <i>C. gloeosporioides</i> and <i>Ph. nicotianae</i>; the diameters of inhibition halos were directly proportional to the extract concentration; values of MIC and MFC of this extract for all pathogens ranged from 2 to 3.5%; the highest concentrations (50 to 100%) tested <i>in vivo</i> were effective in preventing citrus and apple fruit molds. (4) Conclusions: This study contributes to the search for natural and ecofriendly substances for the control of pre- and post-harvest plant pathogens.https://www.mdpi.com/2223-7747/10/11/2452metabolitesphenolic compoundsinhibitory effectcitrusappleHPLC-ESI-MS-TOF |
| spellingShingle | Soumia El boumlasy Federico La Spada Nunzio Tuccitto Giovanni Marletta Carlos Luz Mínguez Giuseppe Meca Ermes Ivan Rovetto Antonella Pane Abderrahmane Debdoubi Santa Olga Cacciola Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens Plants metabolites phenolic compounds inhibitory effect citrus apple HPLC-ESI-MS-TOF |
| title | Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens |
| title_full | Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens |
| title_fullStr | Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens |
| title_full_unstemmed | Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens |
| title_short | Inhibitory Activity of Shrimp Waste Extracts on Fungal and Oomycete Plant Pathogens |
| title_sort | inhibitory activity of shrimp waste extracts on fungal and oomycete plant pathogens |
| topic | metabolites phenolic compounds inhibitory effect citrus apple HPLC-ESI-MS-TOF |
| url | https://www.mdpi.com/2223-7747/10/11/2452 |
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