Sperm quality in frozen beef and dairy bull semen
Abstract Background There is speculation that beef bull semen quality is inferior to that of dairy bulls although few scientific studies are available in the literature. The aim of this study was to evaluate sperm quality in beef bull semen and to determine which parameters could be indicative of fe...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13028-018-0396-2 |
| _version_ | 1818621475904552960 |
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| author | Jane Margaret Morrell Andra Sabina Valeanu Nils Lundeheim Anders Johannisson |
| author_facet | Jane Margaret Morrell Andra Sabina Valeanu Nils Lundeheim Anders Johannisson |
| author_sort | Jane Margaret Morrell |
| collection | DOAJ |
| description | Abstract Background There is speculation that beef bull semen quality is inferior to that of dairy bulls although few scientific studies are available in the literature. The aim of this study was to evaluate sperm quality in beef bull semen and to determine which parameters could be indicative of fertility after insemination. Sperm quality, assessed by computer assisted sperm motility analysis and flow cytometric evaluation of membrane integrity, levels of reactive oxygen species, mitochondrial membrane potential, acrosome status and DNA fragmentation index, was evaluated in beef and dairy bull semen. Results For beef bulls, normal morphology (r = 0.62, P < 0.05) and WOBBLE (r = 0.57, P < 0.05) were significantly correlated with 56-day non-return rate, whereas sperm quality was not significantly correlated with the fertility index score for dairy bulls. Membrane integrity (46 ± 8.0% versus 40 ± 11%, P < 0.05), normal morphology (87 ± 6% versus 76 ± 8%; P < 0.05), and high respiratory activity (52 ± 13 versus 12 ± 4%; P < 0.001) were higher for dairy bulls than for beef bulls. The DNA fragmentation index was lower for dairy bull spermatozoa than beef (3.8 ± 1.1% versus 6.1 ± 2.9%; P < 0.01), whereas some sperm kinematics were higher. Multivariate analysis indicated that type of bull (beef versus dairy) had an impact on sperm quality. Conclusions Different assays of sperm quality may be needed for appropriate analysis of beef and dairy bull semen. These finding could be important for cattle breeding stations when evaluating semen quality. |
| first_indexed | 2024-12-16T18:09:52Z |
| format | Article |
| id | doaj.art-2df6707d265e4bf28e3f6aaab4d9e3a8 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-12-16T18:09:52Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-2df6707d265e4bf28e3f6aaab4d9e3a82022-12-21T22:21:48ZengBMCActa Veterinaria Scandinavica1751-01472018-07-0160111010.1186/s13028-018-0396-2Sperm quality in frozen beef and dairy bull semenJane Margaret Morrell0Andra Sabina Valeanu1Nils Lundeheim2Anders Johannisson3Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU)Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU)Department of Animal Breeding and Genetics, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural SciencesDivision of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU)Abstract Background There is speculation that beef bull semen quality is inferior to that of dairy bulls although few scientific studies are available in the literature. The aim of this study was to evaluate sperm quality in beef bull semen and to determine which parameters could be indicative of fertility after insemination. Sperm quality, assessed by computer assisted sperm motility analysis and flow cytometric evaluation of membrane integrity, levels of reactive oxygen species, mitochondrial membrane potential, acrosome status and DNA fragmentation index, was evaluated in beef and dairy bull semen. Results For beef bulls, normal morphology (r = 0.62, P < 0.05) and WOBBLE (r = 0.57, P < 0.05) were significantly correlated with 56-day non-return rate, whereas sperm quality was not significantly correlated with the fertility index score for dairy bulls. Membrane integrity (46 ± 8.0% versus 40 ± 11%, P < 0.05), normal morphology (87 ± 6% versus 76 ± 8%; P < 0.05), and high respiratory activity (52 ± 13 versus 12 ± 4%; P < 0.001) were higher for dairy bulls than for beef bulls. The DNA fragmentation index was lower for dairy bull spermatozoa than beef (3.8 ± 1.1% versus 6.1 ± 2.9%; P < 0.01), whereas some sperm kinematics were higher. Multivariate analysis indicated that type of bull (beef versus dairy) had an impact on sperm quality. Conclusions Different assays of sperm quality may be needed for appropriate analysis of beef and dairy bull semen. These finding could be important for cattle breeding stations when evaluating semen quality.http://link.springer.com/article/10.1186/s13028-018-0396-2Chromatin integrityMembrane integrityMitochondrial membrane potentialMotilityReactive oxygen species |
| spellingShingle | Jane Margaret Morrell Andra Sabina Valeanu Nils Lundeheim Anders Johannisson Sperm quality in frozen beef and dairy bull semen Acta Veterinaria Scandinavica Chromatin integrity Membrane integrity Mitochondrial membrane potential Motility Reactive oxygen species |
| title | Sperm quality in frozen beef and dairy bull semen |
| title_full | Sperm quality in frozen beef and dairy bull semen |
| title_fullStr | Sperm quality in frozen beef and dairy bull semen |
| title_full_unstemmed | Sperm quality in frozen beef and dairy bull semen |
| title_short | Sperm quality in frozen beef and dairy bull semen |
| title_sort | sperm quality in frozen beef and dairy bull semen |
| topic | Chromatin integrity Membrane integrity Mitochondrial membrane potential Motility Reactive oxygen species |
| url | http://link.springer.com/article/10.1186/s13028-018-0396-2 |
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