Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein
<p>Abstract</p> <p>Background</p> <p>Multiple HIV-1 intersubtype recombinants have been identified in human populations. Previous studies from our lab group have shown that the epidemic in Argentina is characterized by the high prevalence of a circulating recombinant fo...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-10-01
|
| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/7/1/259 |
| _version_ | 1830449750042738688 |
|---|---|
| author | Salomón Horacio Turk Gabriela Ghiglione Yanina Duette Gabriel Espada Constanza De Candia Cristian Carobene Mauricio |
| author_facet | Salomón Horacio Turk Gabriela Ghiglione Yanina Duette Gabriel Espada Constanza De Candia Cristian Carobene Mauricio |
| author_sort | Salomón Horacio |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Multiple HIV-1 intersubtype recombinants have been identified in human populations. Previous studies from our lab group have shown that the epidemic in Argentina is characterized by the high prevalence of a circulating recombinant form, CRF12_BF, and many related BF recombinant forms. In these genomic structures a recombination breakpoint frequently involved the <it>vpu </it>coding region. Due to the scarce knowledge of Vpu participation in the virion release process and its impact on pathogenesis and of the functional capacities of intersubtype recombinant Vpu proteins, the aim of this work was to perform a comparative analysis on virion release capacity and relative replication capacity among viral variants harboring either a BF recombinant Vpu or a subtype B Vpu.</p> <p>Results</p> <p>Our results showed that BF recombinant Vpu was associated to an increased viral particles production when compared to WT B variant in tetherin-expressing cell lines. This observation was tested in the context of a competition assay between the above mentioned variants. The results showed that the replication of the BF Vpu-harboring variant was more efficient in cell cultures than subtype B, reaching a higher frequency in the viral population in a short period of time.</p> <p>Conclusion</p> <p>This study showed that as a result of intersubtype recombination, a structurally re-organized HIV-1 Vpu has an improved <it>in vitro </it>capacity of enhancing viral replication, and provides evidence of the changes occurring in this protein function that could play an important role in the successful spread of intersubtype recombinant variants.</p> |
| first_indexed | 2024-12-21T08:04:11Z |
| format | Article |
| id | doaj.art-2dfdbd8ed8294719a685f6c9f5695e0a |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-21T08:04:11Z |
| publishDate | 2010-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-2dfdbd8ed8294719a685f6c9f5695e0a2022-12-21T19:10:50ZengBMCVirology Journal1743-422X2010-10-017125910.1186/1743-422X-7-259Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu proteinSalomón HoracioTurk GabrielaGhiglione YaninaDuette GabrielEspada ConstanzaDe Candia CristianCarobene Mauricio<p>Abstract</p> <p>Background</p> <p>Multiple HIV-1 intersubtype recombinants have been identified in human populations. Previous studies from our lab group have shown that the epidemic in Argentina is characterized by the high prevalence of a circulating recombinant form, CRF12_BF, and many related BF recombinant forms. In these genomic structures a recombination breakpoint frequently involved the <it>vpu </it>coding region. Due to the scarce knowledge of Vpu participation in the virion release process and its impact on pathogenesis and of the functional capacities of intersubtype recombinant Vpu proteins, the aim of this work was to perform a comparative analysis on virion release capacity and relative replication capacity among viral variants harboring either a BF recombinant Vpu or a subtype B Vpu.</p> <p>Results</p> <p>Our results showed that BF recombinant Vpu was associated to an increased viral particles production when compared to WT B variant in tetherin-expressing cell lines. This observation was tested in the context of a competition assay between the above mentioned variants. The results showed that the replication of the BF Vpu-harboring variant was more efficient in cell cultures than subtype B, reaching a higher frequency in the viral population in a short period of time.</p> <p>Conclusion</p> <p>This study showed that as a result of intersubtype recombination, a structurally re-organized HIV-1 Vpu has an improved <it>in vitro </it>capacity of enhancing viral replication, and provides evidence of the changes occurring in this protein function that could play an important role in the successful spread of intersubtype recombinant variants.</p>http://www.virologyj.com/content/7/1/259 |
| spellingShingle | Salomón Horacio Turk Gabriela Ghiglione Yanina Duette Gabriel Espada Constanza De Candia Cristian Carobene Mauricio Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein Virology Journal |
| title | Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein |
| title_full | Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein |
| title_fullStr | Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein |
| title_full_unstemmed | Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein |
| title_short | Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein |
| title_sort | viral replication is enhanced by an hiv 1 intersubtype recombination derived vpu protein |
| url | http://www.virologyj.com/content/7/1/259 |
| work_keys_str_mv | AT salomonhoracio viralreplicationisenhancedbyanhiv1intersubtyperecombinationderivedvpuprotein AT turkgabriela viralreplicationisenhancedbyanhiv1intersubtyperecombinationderivedvpuprotein AT ghiglioneyanina viralreplicationisenhancedbyanhiv1intersubtyperecombinationderivedvpuprotein AT duettegabriel viralreplicationisenhancedbyanhiv1intersubtyperecombinationderivedvpuprotein AT espadaconstanza viralreplicationisenhancedbyanhiv1intersubtyperecombinationderivedvpuprotein AT decandiacristian viralreplicationisenhancedbyanhiv1intersubtyperecombinationderivedvpuprotein AT carobenemauricio viralreplicationisenhancedbyanhiv1intersubtyperecombinationderivedvpuprotein |