Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine Diets
Pig health is impaired and growth performance is reduced when exposed to deoxynivalenol (DON). The measurement of DON in individual feedstuffs and complete swine diets is variable because of the inconsistent distribution of mycotoxins in feed and the difficulties in obtaining representative samples....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/2/120 |
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| author | Josiane C. Panisson Michael O. Wellington Michael A. Bosompem Veronika Nagl Heidi E. Schwartz-Zimmermann Daniel A. Columbus |
| author_facet | Josiane C. Panisson Michael O. Wellington Michael A. Bosompem Veronika Nagl Heidi E. Schwartz-Zimmermann Daniel A. Columbus |
| author_sort | Josiane C. Panisson |
| collection | DOAJ |
| description | Pig health is impaired and growth performance is reduced when exposed to deoxynivalenol (DON). The measurement of DON in individual feedstuffs and complete swine diets is variable because of the inconsistent distribution of mycotoxins in feed and the difficulties in obtaining representative samples. We investigated whether measuring DON and its metabolites in biological samples could be used as a predictor of DON ingestion by pigs. Blood samples were collected between 3 and 4 h after the morning meal and urine samples were quantitatively collected over a 24 h period on d 40 and 82 of the study to evaluate serum and urinary content of DON and DON metabolites (iso-deoxynivalenol, DON-3-glucuronide, DON-15-glcurunide, deepoxy-deoxynivalenol, iso-deepoxy-deoxynivalenol, deepoxy-deoxynivalenol-3-glucuronide, and deepoxy-deoxynivalenol-15-glucuronide). The intake of DON was positively correlated with urinary DON output. Similarly, there was an increase in serum DON level with increasing DON intake. Overall, it was found that DON intake correlated with DON concentration in urine and blood serum when samples were collected under controlled conditions. Analyzing DON levels in urine and blood serum could be used to predict a pig’s DON intake. |
| first_indexed | 2024-03-11T08:02:34Z |
| format | Article |
| id | doaj.art-2dfe4d339c9744b2be89b9f5f3370922 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-11T08:02:34Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-2dfe4d339c9744b2be89b9f5f33709222023-11-16T23:38:49ZengMDPI AGToxins2072-66512023-02-0115212010.3390/toxins15020120Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine DietsJosiane C. Panisson0Michael O. Wellington1Michael A. Bosompem2Veronika Nagl3Heidi E. Schwartz-Zimmermann4Daniel A. Columbus5Prairie Swine Centre Inc., Saskatoon, SK S7H 5N9, CanadaPrairie Swine Centre Inc., Saskatoon, SK S7H 5N9, CanadaPrairie Swine Centre Inc., Saskatoon, SK S7H 5N9, CanadaDSM-BIOMIN Research Centre, Technopark 1, 3430 Tulln, AustriaInstitute of Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna, 3430 Tulln, AustriaPrairie Swine Centre Inc., Saskatoon, SK S7H 5N9, CanadaPig health is impaired and growth performance is reduced when exposed to deoxynivalenol (DON). The measurement of DON in individual feedstuffs and complete swine diets is variable because of the inconsistent distribution of mycotoxins in feed and the difficulties in obtaining representative samples. We investigated whether measuring DON and its metabolites in biological samples could be used as a predictor of DON ingestion by pigs. Blood samples were collected between 3 and 4 h after the morning meal and urine samples were quantitatively collected over a 24 h period on d 40 and 82 of the study to evaluate serum and urinary content of DON and DON metabolites (iso-deoxynivalenol, DON-3-glucuronide, DON-15-glcurunide, deepoxy-deoxynivalenol, iso-deepoxy-deoxynivalenol, deepoxy-deoxynivalenol-3-glucuronide, and deepoxy-deoxynivalenol-15-glucuronide). The intake of DON was positively correlated with urinary DON output. Similarly, there was an increase in serum DON level with increasing DON intake. Overall, it was found that DON intake correlated with DON concentration in urine and blood serum when samples were collected under controlled conditions. Analyzing DON levels in urine and blood serum could be used to predict a pig’s DON intake.https://www.mdpi.com/2072-6651/15/2/120biomarkersdeoxynivalenoliso-DONDOMmetabolitesserum |
| spellingShingle | Josiane C. Panisson Michael O. Wellington Michael A. Bosompem Veronika Nagl Heidi E. Schwartz-Zimmermann Daniel A. Columbus Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine Diets Toxins biomarkers deoxynivalenol iso-DON DOM metabolites serum |
| title | Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine Diets |
| title_full | Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine Diets |
| title_fullStr | Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine Diets |
| title_full_unstemmed | Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine Diets |
| title_short | Urinary and Serum Concentration of Deoxynivalenol (DON) and DON Metabolites as an Indicator of DON Contamination in Swine Diets |
| title_sort | urinary and serum concentration of deoxynivalenol don and don metabolites as an indicator of don contamination in swine diets |
| topic | biomarkers deoxynivalenol iso-DON DOM metabolites serum |
| url | https://www.mdpi.com/2072-6651/15/2/120 |
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