Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura Nephritis
Background: Purpuric nephritis is the most common secondary glomerular disease in childhood. Its prevalence in children has been steadily rising in recent years. Objective: To explore the characteristics and pathogenesis of changes in peripheral blood lymphocyte subsets and immune function in childr...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2021-09-01
|
| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | https://iji.sums.ac.ir/article_47856_07fe1bbdfc1ca900a37203dd6d6ed3f1.pdf |
| _version_ | 1811286233877315584 |
|---|---|
| author | QingXiao Su LiJun Jiang Jia Chai ZhiYan Dou ZanHua Rong Xue Zhao Bo Yu YuXue Wang XinLiang Wang |
| author_facet | QingXiao Su LiJun Jiang Jia Chai ZhiYan Dou ZanHua Rong Xue Zhao Bo Yu YuXue Wang XinLiang Wang |
| author_sort | QingXiao Su |
| collection | DOAJ |
| description | Background: Purpuric nephritis is the most common secondary glomerular disease in childhood. Its prevalence in children has been steadily rising in recent years. Objective: To explore the characteristics and pathogenesis of changes in peripheral blood lymphocyte subsets and immune function in children with Henoch-Schonlein purpura nephritis. Methods: The study included 104 children with Henoch-Schonlein purpura, divided into nephritis (HSPN) group (68 cases) and non-nephritis (NHSPN) group (36 cases), and 15 normal children. The rate-scatter turbidimetric method was utilized to determine the immunoglobulins IgA, IgG, IgM, C3 and C4, and the flow cytometry technique was employed to detect the levels of lymphocyte subsets including CD3+, CD4+, CD8+, CD4+/CD8+, CD19+, NK, etc. Results: Compared with the control group, the CD3+, CD4+, CD8+ and NK cell levels of peripheral blood mononuclear cells significantly decreased (p <0.05), and the CD19+ level significantly elevated (p <0.05) in the HSPN group and the NHSPN group whereas the HSPN group had a more significant change than the NHSPN group (p <0.05). Compared with the control group, the serum immunoglobulin IgA and IgG of the HSPN group and the NHSPN group significantly increased, and the IgM, C3, and C4 significantly decreased (p <0.05); while the HSPN group had a more significant change than the NHSPN group (p <0.05). Conclusion: Immune dysfunction in children with HSPN is specifically manifested as low cellular immune function, which leads to increased secretion of inflammatory mediators, activates B cells, and further increases the secretion of immunoglobulins, leading to the occurrence of small vasculitis. |
| first_indexed | 2024-04-13T02:55:42Z |
| format | Article |
| id | doaj.art-2dfff38e4eab48cbaf61ff45b75c1eef |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-04-13T02:55:42Z |
| publishDate | 2021-09-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-2dfff38e4eab48cbaf61ff45b75c1eef2022-12-22T03:05:38ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2021-09-0118325926710.22034/iji.2021.89742.196447856Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura NephritisQingXiao Su0LiJun Jiang1Jia Chai2ZhiYan Dou3ZanHua Rong4Xue Zhao5Bo Yu6YuXue Wang7XinLiang Wang8Department of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang, ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang , ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang , ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang , ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang, ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang, ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang , ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang 050000, ChinaDepartment of Peadiatrics, The Second Hospital of HeBei Medical University, ShiJiaZhuang, ChinaBackground: Purpuric nephritis is the most common secondary glomerular disease in childhood. Its prevalence in children has been steadily rising in recent years. Objective: To explore the characteristics and pathogenesis of changes in peripheral blood lymphocyte subsets and immune function in children with Henoch-Schonlein purpura nephritis. Methods: The study included 104 children with Henoch-Schonlein purpura, divided into nephritis (HSPN) group (68 cases) and non-nephritis (NHSPN) group (36 cases), and 15 normal children. The rate-scatter turbidimetric method was utilized to determine the immunoglobulins IgA, IgG, IgM, C3 and C4, and the flow cytometry technique was employed to detect the levels of lymphocyte subsets including CD3+, CD4+, CD8+, CD4+/CD8+, CD19+, NK, etc. Results: Compared with the control group, the CD3+, CD4+, CD8+ and NK cell levels of peripheral blood mononuclear cells significantly decreased (p <0.05), and the CD19+ level significantly elevated (p <0.05) in the HSPN group and the NHSPN group whereas the HSPN group had a more significant change than the NHSPN group (p <0.05). Compared with the control group, the serum immunoglobulin IgA and IgG of the HSPN group and the NHSPN group significantly increased, and the IgM, C3, and C4 significantly decreased (p <0.05); while the HSPN group had a more significant change than the NHSPN group (p <0.05). Conclusion: Immune dysfunction in children with HSPN is specifically manifested as low cellular immune function, which leads to increased secretion of inflammatory mediators, activates B cells, and further increases the secretion of immunoglobulins, leading to the occurrence of small vasculitis.https://iji.sums.ac.ir/article_47856_07fe1bbdfc1ca900a37203dd6d6ed3f1.pdfcomplementhenoch-schonlein purpuraimmunoglobulinlymphocyte subsetspurpuric nephritis |
| spellingShingle | QingXiao Su LiJun Jiang Jia Chai ZhiYan Dou ZanHua Rong Xue Zhao Bo Yu YuXue Wang XinLiang Wang Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura Nephritis Iranian Journal of Immunology complement henoch-schonlein purpura immunoglobulin lymphocyte subsets purpuric nephritis |
| title | Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura Nephritis |
| title_full | Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura Nephritis |
| title_fullStr | Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura Nephritis |
| title_full_unstemmed | Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura Nephritis |
| title_short | Changes of Peripheral Blood Lymphocyte Subsets and Immune Function in Children with Henoch-Schonlein Purpura Nephritis |
| title_sort | changes of peripheral blood lymphocyte subsets and immune function in children with henoch schonlein purpura nephritis |
| topic | complement henoch-schonlein purpura immunoglobulin lymphocyte subsets purpuric nephritis |
| url | https://iji.sums.ac.ir/article_47856_07fe1bbdfc1ca900a37203dd6d6ed3f1.pdf |
| work_keys_str_mv | AT qingxiaosu changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT lijunjiang changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT jiachai changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT zhiyandou changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT zanhuarong changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT xuezhao changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT boyu changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT yuxuewang changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis AT xinliangwang changesofperipheralbloodlymphocytesubsetsandimmunefunctioninchildrenwithhenochschonleinpurpuranephritis |