Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker
SARS-CoV-2 and its mutant strains continue to rapidly spread with high infection and fatality. Large-scale SARS-CoV-2 vaccination provides an important guarantee for effective resistance to existing or mutated SARS-CoV-2 virus infection. However, whether the host metabolite levels respond to SARS-Co...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.954801/full |
| _version_ | 1798000237255589888 |
|---|---|
| author | Maozhang He Yixuan Huang Yun Wang Jiling Liu Maozhen Han Yixuan Xiao Na Zhang Hongya Gui Huan Qiu Liqing Cao Weihua Jia Shenghai Huang Shenghai Huang |
| author_facet | Maozhang He Yixuan Huang Yun Wang Jiling Liu Maozhen Han Yixuan Xiao Na Zhang Hongya Gui Huan Qiu Liqing Cao Weihua Jia Shenghai Huang Shenghai Huang |
| author_sort | Maozhang He |
| collection | DOAJ |
| description | SARS-CoV-2 and its mutant strains continue to rapidly spread with high infection and fatality. Large-scale SARS-CoV-2 vaccination provides an important guarantee for effective resistance to existing or mutated SARS-CoV-2 virus infection. However, whether the host metabolite levels respond to SARS-CoV-2 vaccine-influenced host immunity remains unclear. To help delineate the serum metabolome profile of SARS-CoV-2 vaccinated volunteers and determine that the metabolites tightly respond to host immune antibodies and cytokines, in this study, a total of 59 sera samples were collected from 30 individuals before SARS-CoV-2 vaccination and from 29 COVID-19 vaccines 2 weeks after the two-dose vaccination. Next, untargeted metabolomics was performed and a distinct metabolic composition was revealed between the pre-vaccination (VB) group and two-dose vaccination (SV) group by partial least squares-discriminant and principal component analyses. Based on the criteria: FDR < 0.05, absolute log2 fold change greater than 0.25, and VIP >1, we found that L-glutamic acid, gamma-aminobutyric acid (GABA), succinic acid, and taurine showed increasing trends from SV to VB. Furthermore, SV-associated metabolites were mainly annotated to butanoate metabolism and glutamate metabolism pathways. Moreover, two metabolite biomarkers classified SV from VB individuals with an area under the curve (AUC) of 0.96. Correlation analysis identified a positive association between four metabolites enriched in glutamate metabolism and serum antibodies in relation to IgG, IgM, and IgA. These results suggest that the contents of gamma-aminobutyric acid and indole in serum could be applied as biomarkers in distinguishing vaccinated volunteers from the unvaccinated. What’s more, metabolites such as GABA and taurine may serve as a metabolic target for adjuvant vaccines to boost the ability of the individuals to improve immunity. |
| first_indexed | 2024-04-11T11:17:17Z |
| format | Article |
| id | doaj.art-2e011100a7c6462cab3c1d642203c665 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T11:17:17Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-2e011100a7c6462cab3c1d642203c6652022-12-22T04:27:10ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-09-011310.3389/fimmu.2022.954801954801Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarkerMaozhang He0Yixuan Huang1Yun Wang2Jiling Liu3Maozhen Han4Yixuan Xiao5Na Zhang6Hongya Gui7Huan Qiu8Liqing Cao9Weihua Jia10Shenghai Huang11Shenghai Huang12Department of Microbiology, The Key Laboratory of Microbiology and Parasitology of Anhui Province, The Key Laboratory of Zoonoses of High Institutions in Anhui, School of Basic Medical Sciences, Anhui Medical University, Hefei, ChinaDepartment of Clinical Medicine, The First School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Nosocomial Infection Control, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of Microbiology, The Key Laboratory of Microbiology and Parasitology of Anhui Province, The Key Laboratory of Zoonoses of High Institutions in Anhui, School of Basic Medical Sciences, Anhui Medical University, Hefei, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, ChinaDepartment of Microbiology, The Key Laboratory of Microbiology and Parasitology of Anhui Province, The Key Laboratory of Zoonoses of High Institutions in Anhui, School of Basic Medical Sciences, Anhui Medical University, Hefei, ChinaDepartment of Nosocomial Infection Control, Anhui No.2 Provincial People’s Hospital, Hefei, ChinaDepartment of Microbiology, The Key Laboratory of Microbiology and Parasitology of Anhui Province, The Key Laboratory of Zoonoses of High Institutions in Anhui, School of Basic Medical Sciences, Anhui Medical University, Hefei, ChinaSchool of Nursing, Anhui Medical University, Hefei, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, ChinaDepartment of Microbiology, The Key Laboratory of Microbiology and Parasitology of Anhui Province, The Key Laboratory of Zoonoses of High Institutions in Anhui, School of Basic Medical Sciences, Anhui Medical University, Hefei, ChinaSchool of Life Sciences, Anhui Medical University, Hefei, ChinaSARS-CoV-2 and its mutant strains continue to rapidly spread with high infection and fatality. Large-scale SARS-CoV-2 vaccination provides an important guarantee for effective resistance to existing or mutated SARS-CoV-2 virus infection. However, whether the host metabolite levels respond to SARS-CoV-2 vaccine-influenced host immunity remains unclear. To help delineate the serum metabolome profile of SARS-CoV-2 vaccinated volunteers and determine that the metabolites tightly respond to host immune antibodies and cytokines, in this study, a total of 59 sera samples were collected from 30 individuals before SARS-CoV-2 vaccination and from 29 COVID-19 vaccines 2 weeks after the two-dose vaccination. Next, untargeted metabolomics was performed and a distinct metabolic composition was revealed between the pre-vaccination (VB) group and two-dose vaccination (SV) group by partial least squares-discriminant and principal component analyses. Based on the criteria: FDR < 0.05, absolute log2 fold change greater than 0.25, and VIP >1, we found that L-glutamic acid, gamma-aminobutyric acid (GABA), succinic acid, and taurine showed increasing trends from SV to VB. Furthermore, SV-associated metabolites were mainly annotated to butanoate metabolism and glutamate metabolism pathways. Moreover, two metabolite biomarkers classified SV from VB individuals with an area under the curve (AUC) of 0.96. Correlation analysis identified a positive association between four metabolites enriched in glutamate metabolism and serum antibodies in relation to IgG, IgM, and IgA. These results suggest that the contents of gamma-aminobutyric acid and indole in serum could be applied as biomarkers in distinguishing vaccinated volunteers from the unvaccinated. What’s more, metabolites such as GABA and taurine may serve as a metabolic target for adjuvant vaccines to boost the ability of the individuals to improve immunity.https://www.frontiersin.org/articles/10.3389/fimmu.2022.954801/fullCOVID-19SARS-CoV-2 vaccinationserumantibodies and cytokinesmetabolomic analysis |
| spellingShingle | Maozhang He Yixuan Huang Yun Wang Jiling Liu Maozhen Han Yixuan Xiao Na Zhang Hongya Gui Huan Qiu Liqing Cao Weihua Jia Shenghai Huang Shenghai Huang Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker Frontiers in Immunology COVID-19 SARS-CoV-2 vaccination serum antibodies and cytokines metabolomic analysis |
| title | Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker |
| title_full | Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker |
| title_fullStr | Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker |
| title_full_unstemmed | Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker |
| title_short | Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker |
| title_sort | metabolomics based investigation of sars cov 2 vaccination sinovac reveals an immune dependent metabolite biomarker |
| topic | COVID-19 SARS-CoV-2 vaccination serum antibodies and cytokines metabolomic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.954801/full |
| work_keys_str_mv | AT maozhanghe metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT yixuanhuang metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT yunwang metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT jilingliu metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT maozhenhan metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT yixuanxiao metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT nazhang metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT hongyagui metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT huanqiu metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT liqingcao metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT weihuajia metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT shenghaihuang metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker AT shenghaihuang metabolomicsbasedinvestigationofsarscov2vaccinationsinovacrevealsanimmunedependentmetabolitebiomarker |