Upregulation of GLRs expression by light in Arabidopsis leaves
Abstract Background Glutamate receptor-like (GLR) channels are plant homologs of iGluRs, animal ionotropic glutamate receptors which participate in neurotransmission. GLRs mediate plant adaptive processes and photomorphogenesis. Despite their contribution to light-dependent processes, signaling mech...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-04-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03535-7 |
| _version_ | 1811303058223661056 |
|---|---|
| author | Anna Hebda Aleksandra Liszka Aleksandra Lewandowska Jan J. Lyczakowski Halina Gabryś Weronika Krzeszowiec |
| author_facet | Anna Hebda Aleksandra Liszka Aleksandra Lewandowska Jan J. Lyczakowski Halina Gabryś Weronika Krzeszowiec |
| author_sort | Anna Hebda |
| collection | DOAJ |
| description | Abstract Background Glutamate receptor-like (GLR) channels are plant homologs of iGluRs, animal ionotropic glutamate receptors which participate in neurotransmission. GLRs mediate plant adaptive processes and photomorphogenesis. Despite their contribution to light-dependent processes, signaling mechanisms that modulate GLR response to light remain unknown. Here we show that leaf expression of 7 out of 20 Arabidopsis GLRs is significantly up-regulated by monochromatic irradiation. Results Our data indicates that both red and blue light stimulate the expression of selected AtGLRs. Using a photosynthesis inhibitor and different irradiation regimes, we demonstrated that retrograde signaling from photosystem II is unlikely to be involved in light-dependent GLR up-regulation. Analysis of transcriptional patterns in mutants of key photoreceptors allowed us to observe that both phytochromes and cryptochromes are likely to be involved in the control of light-dependent up-regulation of AtGLR expression, with phytochromes playing a clearly dominating role in this process. Conclusions In mature Arabidopsis leaves, phytochromes, assisted by cryptochromes, mediate light-driven transcriptional up-regulation of several genes encoding GLR proteins. Since GLRs are known to be involved in a wide range of plant developmental processes our results provide mechanistic insight into how light may influence plant growth and development. |
| first_indexed | 2024-04-13T07:39:55Z |
| format | Article |
| id | doaj.art-2e0348aa0bb3412cbacdbabbb9a76fa2 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T07:39:55Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-2e0348aa0bb3412cbacdbabbb9a76fa22022-12-22T02:55:56ZengBMCBMC Plant Biology1471-22292022-04-0122111110.1186/s12870-022-03535-7Upregulation of GLRs expression by light in Arabidopsis leavesAnna Hebda0Aleksandra Liszka1Aleksandra Lewandowska2Jan J. Lyczakowski3Halina Gabryś4Weronika Krzeszowiec5Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in KrakówDepartment of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in KrakówDepartment of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in KrakówDepartment of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in KrakówDepartment of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in KrakówDepartment of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in KrakówAbstract Background Glutamate receptor-like (GLR) channels are plant homologs of iGluRs, animal ionotropic glutamate receptors which participate in neurotransmission. GLRs mediate plant adaptive processes and photomorphogenesis. Despite their contribution to light-dependent processes, signaling mechanisms that modulate GLR response to light remain unknown. Here we show that leaf expression of 7 out of 20 Arabidopsis GLRs is significantly up-regulated by monochromatic irradiation. Results Our data indicates that both red and blue light stimulate the expression of selected AtGLRs. Using a photosynthesis inhibitor and different irradiation regimes, we demonstrated that retrograde signaling from photosystem II is unlikely to be involved in light-dependent GLR up-regulation. Analysis of transcriptional patterns in mutants of key photoreceptors allowed us to observe that both phytochromes and cryptochromes are likely to be involved in the control of light-dependent up-regulation of AtGLR expression, with phytochromes playing a clearly dominating role in this process. Conclusions In mature Arabidopsis leaves, phytochromes, assisted by cryptochromes, mediate light-driven transcriptional up-regulation of several genes encoding GLR proteins. Since GLRs are known to be involved in a wide range of plant developmental processes our results provide mechanistic insight into how light may influence plant growth and development.https://doi.org/10.1186/s12870-022-03535-7Blue lightCryptochromeGLRPhototropinsPhytochromesRed/far red light |
| spellingShingle | Anna Hebda Aleksandra Liszka Aleksandra Lewandowska Jan J. Lyczakowski Halina Gabryś Weronika Krzeszowiec Upregulation of GLRs expression by light in Arabidopsis leaves BMC Plant Biology Blue light Cryptochrome GLR Phototropins Phytochromes Red/far red light |
| title | Upregulation of GLRs expression by light in Arabidopsis leaves |
| title_full | Upregulation of GLRs expression by light in Arabidopsis leaves |
| title_fullStr | Upregulation of GLRs expression by light in Arabidopsis leaves |
| title_full_unstemmed | Upregulation of GLRs expression by light in Arabidopsis leaves |
| title_short | Upregulation of GLRs expression by light in Arabidopsis leaves |
| title_sort | upregulation of glrs expression by light in arabidopsis leaves |
| topic | Blue light Cryptochrome GLR Phototropins Phytochromes Red/far red light |
| url | https://doi.org/10.1186/s12870-022-03535-7 |
| work_keys_str_mv | AT annahebda upregulationofglrsexpressionbylightinarabidopsisleaves AT aleksandraliszka upregulationofglrsexpressionbylightinarabidopsisleaves AT aleksandralewandowska upregulationofglrsexpressionbylightinarabidopsisleaves AT janjlyczakowski upregulationofglrsexpressionbylightinarabidopsisleaves AT halinagabrys upregulationofglrsexpressionbylightinarabidopsisleaves AT weronikakrzeszowiec upregulationofglrsexpressionbylightinarabidopsisleaves |