Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments
During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implement...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/12/2413 |
| _version_ | 1797499955678543872 |
|---|---|
| author | Martina Pavletić Marija Mazor Mate Lerga Tatjana Mileta Jelena Železnjak Tina Ružić Sanda Ravlić Dora Palčevski Jelena Kirinčić Silvestar Mežnarić Ana Mišković Maja Materljan Alan Šustić Berislav Lisnić Vanda Juranić Lisnić |
| author_facet | Martina Pavletić Marija Mazor Mate Lerga Tatjana Mileta Jelena Železnjak Tina Ružić Sanda Ravlić Dora Palčevski Jelena Kirinčić Silvestar Mežnarić Ana Mišković Maja Materljan Alan Šustić Berislav Lisnić Vanda Juranić Lisnić |
| author_sort | Martina Pavletić |
| collection | DOAJ |
| description | During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR. |
| first_indexed | 2024-03-10T03:54:55Z |
| format | Article |
| id | doaj.art-2e03856b8f434e16b3b8f72c0b81dfb1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T03:54:55Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-2e03856b8f434e16b3b8f72c0b81dfb12023-11-23T10:57:35ZengMDPI AGViruses1999-49152021-12-011312241310.3390/v13122413Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency DepartmentsMartina Pavletić0Marija Mazor1Mate Lerga2Tatjana Mileta3Jelena Železnjak4Tina Ružić5Sanda Ravlić6Dora Palčevski7Jelena Kirinčić8Silvestar Mežnarić9Ana Mišković10Maja Materljan11Alan Šustić12Berislav Lisnić13Vanda Juranić Lisnić14Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCentre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, 10000 Zagreb, CroatiaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, CroatiaDepartment of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, CroatiaDuring COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.https://www.mdpi.com/1999-4915/13/12/2413SARS-CoV-2qPCRdirect qPCRdqPCRpoint-of-care PCRemergency department |
| spellingShingle | Martina Pavletić Marija Mazor Mate Lerga Tatjana Mileta Jelena Železnjak Tina Ružić Sanda Ravlić Dora Palčevski Jelena Kirinčić Silvestar Mežnarić Ana Mišković Maja Materljan Alan Šustić Berislav Lisnić Vanda Juranić Lisnić Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments Viruses SARS-CoV-2 qPCR direct qPCR dqPCR point-of-care PCR emergency department |
| title | Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments |
| title_full | Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments |
| title_fullStr | Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments |
| title_full_unstemmed | Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments |
| title_short | Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments |
| title_sort | fast reliable and simple point of care like adaptation of rt qpcr for the detection of sars cov 2 for use in hospital emergency departments |
| topic | SARS-CoV-2 qPCR direct qPCR dqPCR point-of-care PCR emergency department |
| url | https://www.mdpi.com/1999-4915/13/12/2413 |
| work_keys_str_mv | AT martinapavletic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT marijamazor fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT matelerga fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT tatjanamileta fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT jelenazeleznjak fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT tinaruzic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT sandaravlic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT dorapalcevski fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT jelenakirincic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT silvestarmeznaric fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT anamiskovic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT majamaterljan fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT alansustic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT berislavlisnic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments AT vandajuraniclisnic fastreliableandsimplepointofcarelikeadaptationofrtqpcrforthedetectionofsarscov2foruseinhospitalemergencydepartments |