The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture
The aim of this study was to investigate the relationship between the resistance of Verticillium wilt and the amount of phytoalexin, which is the defense molecules synthesized in plant tissues. In this study, Long Purple eggplant variety (disease susceptible) and S. sisymbriifolium wild type (diseas...
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Format: | Article |
Language: | English |
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Bati Akdeniz Agricultural Research Institute
2019-12-01
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Series: | Derim |
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Online Access: | http://www.derim.com.tr/tr/download/article-file/878731 |
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author | Sevinç KIRAN S.Şebnem ELLİALTIOĞLU |
author_facet | Sevinç KIRAN S.Şebnem ELLİALTIOĞLU |
author_sort | Sevinç KIRAN |
collection | DOAJ |
description | The aim of this study was to investigate the relationship between the resistance of Verticillium wilt and the amount of phytoalexin, which is the defense molecules synthesized in plant tissues. In this study, Long Purple eggplant variety (disease susceptible) and S. sisymbriifolium wild type (disease resistant) were used as plant material. As elicitor, arachidonic acid (5 and 10 mM) was included in the study. Stimulation with elicitor lasted 24, 48 and 72 hours. Extracted substances were analyzed in gas liquid chromatography (GLC). The formation of phytoalexin was not adequately stimulated in the callus suspension culture of the resistant species. The results showed that arachidonic acid was effective in stimulating the formation of phytoalexins in the callus suspension culture in Long Purple variety, and in the in vitro conditions solavetivone was formed in the tissue phase and lubimin substances in the filtrate phase. The highest material accumulations were obtained in both phases: ’10 mM arachidonic acid x 72 hours’ ‘elicitor dose x stimulation period’ interaction was found to be significant. |
first_indexed | 2024-04-10T13:39:54Z |
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id | doaj.art-2e03e82274514417ac7d4d726d0af0f8 |
institution | Directory Open Access Journal |
issn | 1300-3496 1300-3496 |
language | English |
last_indexed | 2024-04-10T13:39:54Z |
publishDate | 2019-12-01 |
publisher | Bati Akdeniz Agricultural Research Institute |
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series | Derim |
spelling | doaj.art-2e03e82274514417ac7d4d726d0af0f82023-02-15T16:11:14ZengBati Akdeniz Agricultural Research InstituteDerim1300-34961300-34962019-12-0136214114510.16882/derim.2019.611536The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension cultureSevinç KIRANS.Şebnem ELLİALTIOĞLUThe aim of this study was to investigate the relationship between the resistance of Verticillium wilt and the amount of phytoalexin, which is the defense molecules synthesized in plant tissues. In this study, Long Purple eggplant variety (disease susceptible) and S. sisymbriifolium wild type (disease resistant) were used as plant material. As elicitor, arachidonic acid (5 and 10 mM) was included in the study. Stimulation with elicitor lasted 24, 48 and 72 hours. Extracted substances were analyzed in gas liquid chromatography (GLC). The formation of phytoalexin was not adequately stimulated in the callus suspension culture of the resistant species. The results showed that arachidonic acid was effective in stimulating the formation of phytoalexins in the callus suspension culture in Long Purple variety, and in the in vitro conditions solavetivone was formed in the tissue phase and lubimin substances in the filtrate phase. The highest material accumulations were obtained in both phases: ’10 mM arachidonic acid x 72 hours’ ‘elicitor dose x stimulation period’ interaction was found to be significant.http://www.derim.com.tr/tr/download/article-file/878731callus cultureeggplantlubiminresistancesolavetivone |
spellingShingle | Sevinç KIRAN S.Şebnem ELLİALTIOĞLU The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture Derim callus culture eggplant lubimin resistance solavetivone |
title | The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture |
title_full | The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture |
title_fullStr | The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture |
title_full_unstemmed | The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture |
title_short | The effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture |
title_sort | effect of arachidonic acid elicitor on phytoalexin stimulation in eggplant callus suspension culture |
topic | callus culture eggplant lubimin resistance solavetivone |
url | http://www.derim.com.tr/tr/download/article-file/878731 |
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