Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from Indonesia
<i>Monochoria vaginalis</i> (Burm. f.) C. Presl, belonging to the family Pontederiaceae, is an aquatic herbaceous plant, native to temperate and tropical Asia. The species often occurs in paddy fields as a noxious weed in East Asia, and in the USA, and causes a significant reduction in r...
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MDPI AG
2022-01-01
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| author | Ryan Widianto Denny Kurniadie Dedi Widayat Uum Umiyati Ceppy Nasahi Santika Sari Abdul Shukor Juraimi Hisashi Kato-Noguchi |
| author_facet | Ryan Widianto Denny Kurniadie Dedi Widayat Uum Umiyati Ceppy Nasahi Santika Sari Abdul Shukor Juraimi Hisashi Kato-Noguchi |
| author_sort | Ryan Widianto |
| collection | DOAJ |
| description | <i>Monochoria vaginalis</i> (Burm. f.) C. Presl, belonging to the family Pontederiaceae, is an aquatic herbaceous plant, native to temperate and tropical Asia. The species often occurs in paddy fields as a noxious weed in East Asia, and in the USA, and causes a significant reduction in rice production. The objective of the present research was the evaluation of the resistance levels of <i>M. vaginalis</i> against three chemical groups of acetolactate synthase (ALS)-inhibitor herbicides and other two different groups of herbicides, and the investigation of the mutations in the <i>ALS</i> gene of the resistant biotype of <i>M. vaginalis</i>. Herbicide dose–response experiments showed that the resistant biotype of <i>M. vaginalis</i> was highly resistant to bensulfuron-methyl, moderately resistant to bispyribac-sodium, had low resistance to penoxsulam and 2,4-D dimethyl ammonium, and was susceptible to sulfentrazone. The nucleotide sequences of the <i>ALS gene</i> of resistant and susceptible biotypes showed 14 base substitutions, which caused two amino acid substitutions: Val-143-Ile and Val-148-Ile. It is the first report of the substitutions of amino acids Val-143-Ile and Val-148-Ile in ALS protein. Those mutations may give different resistance spectra against three ALS-inhibitor herbicides: bensulfuron-methyl, bispyribac-sodium, and penoxsulam. Further research is needed to elucidate the molecular basis of target-site resistance mechanisms such as the transformation of the <i>ALS</i> gene of <i>M. vaginalis</i>. It is also necessary to evaluate herbicide mixtures and/or the rotation of herbicide sites of action to control the resistant biotype of <i>M. vaginalis.</i> |
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| publishDate | 2022-01-01 |
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| spelling | doaj.art-2e04ca0f61af4c9299a1796a355b31832023-11-23T17:31:21ZengMDPI AGPlants2223-77472022-01-0111340010.3390/plants11030400Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from IndonesiaRyan Widianto0Denny Kurniadie1Dedi Widayat2Uum Umiyati3Ceppy Nasahi4Santika Sari5Abdul Shukor Juraimi6Hisashi Kato-Noguchi7Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya, Bandung Sumedang Km 21, Jatinangor, Sumedang 45363, Jawa Barat, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya, Bandung Sumedang Km 21, Jatinangor, Sumedang 45363, Jawa Barat, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya, Bandung Sumedang Km 21, Jatinangor, Sumedang 45363, Jawa Barat, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya, Bandung Sumedang Km 21, Jatinangor, Sumedang 45363, Jawa Barat, IndonesiaDepartment of Pest and Diseases, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya, Bandung Sumedang Km 21, Jatinangor, Sumedang 45363, Jawa Barat, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya, Bandung Sumedang Km 21, Jatinangor, Sumedang 45363, Jawa Barat, IndonesiaDepartment of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki 761-0795, Kagawa, Japan<i>Monochoria vaginalis</i> (Burm. f.) C. Presl, belonging to the family Pontederiaceae, is an aquatic herbaceous plant, native to temperate and tropical Asia. The species often occurs in paddy fields as a noxious weed in East Asia, and in the USA, and causes a significant reduction in rice production. The objective of the present research was the evaluation of the resistance levels of <i>M. vaginalis</i> against three chemical groups of acetolactate synthase (ALS)-inhibitor herbicides and other two different groups of herbicides, and the investigation of the mutations in the <i>ALS</i> gene of the resistant biotype of <i>M. vaginalis</i>. Herbicide dose–response experiments showed that the resistant biotype of <i>M. vaginalis</i> was highly resistant to bensulfuron-methyl, moderately resistant to bispyribac-sodium, had low resistance to penoxsulam and 2,4-D dimethyl ammonium, and was susceptible to sulfentrazone. The nucleotide sequences of the <i>ALS gene</i> of resistant and susceptible biotypes showed 14 base substitutions, which caused two amino acid substitutions: Val-143-Ile and Val-148-Ile. It is the first report of the substitutions of amino acids Val-143-Ile and Val-148-Ile in ALS protein. Those mutations may give different resistance spectra against three ALS-inhibitor herbicides: bensulfuron-methyl, bispyribac-sodium, and penoxsulam. Further research is needed to elucidate the molecular basis of target-site resistance mechanisms such as the transformation of the <i>ALS</i> gene of <i>M. vaginalis</i>. It is also necessary to evaluate herbicide mixtures and/or the rotation of herbicide sites of action to control the resistant biotype of <i>M. vaginalis.</i>https://www.mdpi.com/2223-7747/11/3/400acetolactate synthaseherbicide resistance<i>Monochoria vaginalis</i>DNAresistant spectrum |
| spellingShingle | Ryan Widianto Denny Kurniadie Dedi Widayat Uum Umiyati Ceppy Nasahi Santika Sari Abdul Shukor Juraimi Hisashi Kato-Noguchi Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from Indonesia Plants acetolactate synthase herbicide resistance <i>Monochoria vaginalis</i> DNA resistant spectrum |
| title | Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from Indonesia |
| title_full | Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from Indonesia |
| title_fullStr | Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from Indonesia |
| title_full_unstemmed | Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from Indonesia |
| title_short | Acetolactate Synthase-Inhibitor Resistance in <i>Monochoria vaginalis</i> (Burm. f.) C. Presl from Indonesia |
| title_sort | acetolactate synthase inhibitor resistance in i monochoria vaginalis i burm f c presl from indonesia |
| topic | acetolactate synthase herbicide resistance <i>Monochoria vaginalis</i> DNA resistant spectrum |
| url | https://www.mdpi.com/2223-7747/11/3/400 |
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