Feasible choices in diagnostic methods of malaria
Background & objectives: The present study was undertaken to find out a new easy method in thediagnosis of malaria by centrifuged buffy coat smear, which was found to be a feasible and reasonableprocedure.Methods: Blood samples collected from 120 patients suspected of malaria were subjected to a...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2010-09-01
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| Series: | Journal of Vector Borne Diseases |
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| Online Access: | http://www.mrcindia.org/journal/issues/473151.pdf |
| _version_ | 1818537977158041600 |
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| author | Shamim Akhtar, Sabiha Maimoon, Anne Wilkinson, Vidula Gowardhan , Sadhana Mahore |
| author_facet | Shamim Akhtar, Sabiha Maimoon, Anne Wilkinson, Vidula Gowardhan , Sadhana Mahore |
| author_sort | Shamim Akhtar, Sabiha Maimoon, Anne Wilkinson, Vidula Gowardhan , Sadhana Mahore |
| collection | DOAJ |
| description | Background & objectives: The present study was undertaken to find out a new easy method in thediagnosis of malaria by centrifuged buffy coat smear, which was found to be a feasible and reasonableprocedure.Methods: Blood samples collected from 120 patients suspected of malaria were subjected to allthree diagnostic modalities—peripheral blood smear (PS), centrifuged buffy coat smear (CBCS)and antigen detection test using pLDH and aldolase (AG).Results: The results of various methods were compared. It was seen that addition of centrifugation(i.e. CBCS) to conventional method of PS (i.e. thick and thin smears) improved its sensitivityfrom 85 to 93.3%. Antigen detection and CBCS were found superior to PS in sensitivity. CBCSgives combined sensitivity and specificity of both antigen and PS.Conclusion: CBCS is as sensitive as antigen test and as specific as PS in species identification. Itis a reasonable and feasible procedure too. |
| first_indexed | 2024-12-11T18:57:39Z |
| format | Article |
| id | doaj.art-2e05a3afe94c4afa9fd745a9a618cfe1 |
| institution | Directory Open Access Journal |
| issn | 0972-9062 |
| language | English |
| last_indexed | 2024-12-11T18:57:39Z |
| publishDate | 2010-09-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Vector Borne Diseases |
| spelling | doaj.art-2e05a3afe94c4afa9fd745a9a618cfe12022-12-22T00:54:06ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90622010-09-01473151154Feasible choices in diagnostic methods of malariaShamim Akhtar, Sabiha Maimoon, Anne Wilkinson, Vidula Gowardhan , Sadhana MahoreBackground & objectives: The present study was undertaken to find out a new easy method in thediagnosis of malaria by centrifuged buffy coat smear, which was found to be a feasible and reasonableprocedure.Methods: Blood samples collected from 120 patients suspected of malaria were subjected to allthree diagnostic modalities—peripheral blood smear (PS), centrifuged buffy coat smear (CBCS)and antigen detection test using pLDH and aldolase (AG).Results: The results of various methods were compared. It was seen that addition of centrifugation(i.e. CBCS) to conventional method of PS (i.e. thick and thin smears) improved its sensitivityfrom 85 to 93.3%. Antigen detection and CBCS were found superior to PS in sensitivity. CBCSgives combined sensitivity and specificity of both antigen and PS.Conclusion: CBCS is as sensitive as antigen test and as specific as PS in species identification. Itis a reasonable and feasible procedure too.http://www.mrcindia.org/journal/issues/473151.pdfBuffy coat smeardiagnostic methodsmalariaperipheral blood smear |
| spellingShingle | Shamim Akhtar, Sabiha Maimoon, Anne Wilkinson, Vidula Gowardhan , Sadhana Mahore Feasible choices in diagnostic methods of malaria Journal of Vector Borne Diseases Buffy coat smear diagnostic methods malaria peripheral blood smear |
| title | Feasible choices in diagnostic methods of malaria |
| title_full | Feasible choices in diagnostic methods of malaria |
| title_fullStr | Feasible choices in diagnostic methods of malaria |
| title_full_unstemmed | Feasible choices in diagnostic methods of malaria |
| title_short | Feasible choices in diagnostic methods of malaria |
| title_sort | feasible choices in diagnostic methods of malaria |
| topic | Buffy coat smear diagnostic methods malaria peripheral blood smear |
| url | http://www.mrcindia.org/journal/issues/473151.pdf |
| work_keys_str_mv | AT shamimakhtarsabihamaimoonannewilkinsonvidulagowardhansadhanamahore feasiblechoicesindiagnosticmethodsofmalaria |