Fusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene Accumulation
The gene Tri12 encodes a predicted major facilitator superfamily protein suggested to play a role in export of trichothecene mycotoxins produced by Fusarium spp. It is unclear, however, how the Tri12 protein (Tri12p) may influence trichothecene sensitivity and virulence of the wheat pathogen Fusariu...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2012-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-12-0081-R |
| _version_ | 1811291315963428864 |
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| author | Jon Menke Yanhong Dong H. Corby Kistler |
| author_facet | Jon Menke Yanhong Dong H. Corby Kistler |
| author_sort | Jon Menke |
| collection | DOAJ |
| description | The gene Tri12 encodes a predicted major facilitator superfamily protein suggested to play a role in export of trichothecene mycotoxins produced by Fusarium spp. It is unclear, however, how the Tri12 protein (Tri12p) may influence trichothecene sensitivity and virulence of the wheat pathogen Fusarium graminearum. In this study, we establish a role for Tri12 in toxin accumulation and sensitivity as well as in pathogenicity toward wheat. Tri12 deletion mutants (tri12) are reduced in virulence and result in decreased trichothecene accumulation when inoculated on wheat compared with the wild-type strain or an ectopic mutant. Reduced radial growth of tri12 mutants on trichothecene biosynthesis induction medium was observed relative to the wild type and the ectopic strains. Diminished trichothecene accumulation was observed in liquid medium cultures inoculated with tri12 mutants. Wild-type fungal cells grown under conditions that induce trichothecene biosynthesis develop distinct subapical swelling and form large vacuoles. A strain expressing Tri12p linked to green fluorescent protein shows localization of the protein consistent with the plasma membrane. Our results indicate Tri12 plays a role in self-protection and influences toxin production and virulence of the fungus in planta. |
| first_indexed | 2024-04-13T04:27:28Z |
| format | Article |
| id | doaj.art-2e06b8dcfc724ec1b97d28176771a88d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:27:28Z |
| publishDate | 2012-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-2e06b8dcfc724ec1b97d28176771a88d2022-12-22T03:02:26ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062012-11-0125111408141810.1094/MPMI-04-12-0081-RFusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene AccumulationJon MenkeYanhong DongH. Corby KistlerThe gene Tri12 encodes a predicted major facilitator superfamily protein suggested to play a role in export of trichothecene mycotoxins produced by Fusarium spp. It is unclear, however, how the Tri12 protein (Tri12p) may influence trichothecene sensitivity and virulence of the wheat pathogen Fusarium graminearum. In this study, we establish a role for Tri12 in toxin accumulation and sensitivity as well as in pathogenicity toward wheat. Tri12 deletion mutants (tri12) are reduced in virulence and result in decreased trichothecene accumulation when inoculated on wheat compared with the wild-type strain or an ectopic mutant. Reduced radial growth of tri12 mutants on trichothecene biosynthesis induction medium was observed relative to the wild type and the ectopic strains. Diminished trichothecene accumulation was observed in liquid medium cultures inoculated with tri12 mutants. Wild-type fungal cells grown under conditions that induce trichothecene biosynthesis develop distinct subapical swelling and form large vacuoles. A strain expressing Tri12p linked to green fluorescent protein shows localization of the protein consistent with the plasma membrane. Our results indicate Tri12 plays a role in self-protection and influences toxin production and virulence of the fungus in planta.https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-12-0081-R |
| spellingShingle | Jon Menke Yanhong Dong H. Corby Kistler Fusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene Accumulation Molecular Plant-Microbe Interactions |
| title | Fusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene Accumulation |
| title_full | Fusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene Accumulation |
| title_fullStr | Fusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene Accumulation |
| title_full_unstemmed | Fusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene Accumulation |
| title_short | Fusarium graminearum Tri12p Influences Virulence to Wheat and Trichothecene Accumulation |
| title_sort | fusarium graminearum tri12p influences virulence to wheat and trichothecene accumulation |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-12-0081-R |
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