Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patients
Background: The etiology of Multiple sclerosis (MS) is complicated and can be affected by several environmental factors, such as Mycobacterium avium subspecies paratuberculosis (MAP) infection in genetically predisposed individuals. The link between MAP and MS depends on host genetic and epigenetic...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023093453 |
| _version_ | 1797429678385922048 |
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| author | Negar Asgari Ezzat Allah Ghaemi Mohammad Hasan Naeimi Alireza Tahamtan Leonardo Antonio Sechi Samin Zamani |
| author_facet | Negar Asgari Ezzat Allah Ghaemi Mohammad Hasan Naeimi Alireza Tahamtan Leonardo Antonio Sechi Samin Zamani |
| author_sort | Negar Asgari |
| collection | DOAJ |
| description | Background: The etiology of Multiple sclerosis (MS) is complicated and can be affected by several environmental factors, such as Mycobacterium avium subspecies paratuberculosis (MAP) infection in genetically predisposed individuals. The link between MAP and MS depends on host genetic and epigenetic aspects and population-based features that require further investigation. We aimed to study the possible role of MAP in triggering MS using molecular and serological methods. Materials and methods: This case-control study examined 200 blood samples (100 MS patients and 100 HCs) to search for the MAP-specific IS900 gene. In addition, ELISA was conducted to determine the humoral response against MAP_402718-32 and its human IRF5424–434 peptide homolog. Results: The frequency of MAP detection based on the molecular method in MS patients and HCs was 48 % and 13 %, respectively (p < 0.0001). The presence of antibodies against MAP_402718-32 and IRF5424–434 was 55 % and 65 % in MS patients versus 9 % and 7 % in HCs, respectively (p < 0.0001). A good correlation was observed between MAP_4027 and IRF5 antibodies (r = 0.5782, p < 0.0001), indicating that the same antibodies recognized common peptide epitopes. Conclusion: Our research revealed a significant association between MAP and MS, highlighting the possible role of MAP as an important infection trigger factor of MS. It is hypothesized that cross-reactivity between MAP4027 and IRF5 may dysregulate immune homeostasis. |
| first_indexed | 2024-03-09T09:16:37Z |
| format | Article |
| id | doaj.art-2e07a1a7bc40438398839dd95a99fca4 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:16:37Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-2e07a1a7bc40438398839dd95a99fca42023-12-02T07:05:17ZengElsevierHeliyon2405-84402023-11-01911e22137Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patientsNegar Asgari0Ezzat Allah Ghaemi1Mohammad Hasan Naeimi2Alireza Tahamtan3Leonardo Antonio Sechi4Samin Zamani5Neuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranDepartment of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranDepartment of Neurology, Sayyad Hospital, Golestan University of Medical Sciences, Gorgan, IranDepartment of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranAzienda Ospedaliera Universitaria, Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43b, 07100, Sassari, ItalyNeuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran; Corresponding author. Dept. of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.Background: The etiology of Multiple sclerosis (MS) is complicated and can be affected by several environmental factors, such as Mycobacterium avium subspecies paratuberculosis (MAP) infection in genetically predisposed individuals. The link between MAP and MS depends on host genetic and epigenetic aspects and population-based features that require further investigation. We aimed to study the possible role of MAP in triggering MS using molecular and serological methods. Materials and methods: This case-control study examined 200 blood samples (100 MS patients and 100 HCs) to search for the MAP-specific IS900 gene. In addition, ELISA was conducted to determine the humoral response against MAP_402718-32 and its human IRF5424–434 peptide homolog. Results: The frequency of MAP detection based on the molecular method in MS patients and HCs was 48 % and 13 %, respectively (p < 0.0001). The presence of antibodies against MAP_402718-32 and IRF5424–434 was 55 % and 65 % in MS patients versus 9 % and 7 % in HCs, respectively (p < 0.0001). A good correlation was observed between MAP_4027 and IRF5 antibodies (r = 0.5782, p < 0.0001), indicating that the same antibodies recognized common peptide epitopes. Conclusion: Our research revealed a significant association between MAP and MS, highlighting the possible role of MAP as an important infection trigger factor of MS. It is hypothesized that cross-reactivity between MAP4027 and IRF5 may dysregulate immune homeostasis.http://www.sciencedirect.com/science/article/pii/S2405844023093453Mycobacterium avium subspecies paratuberculosisMultiple sclerosisInterferon regulatory factor 5 proteinHumanELISANested PCR |
| spellingShingle | Negar Asgari Ezzat Allah Ghaemi Mohammad Hasan Naeimi Alireza Tahamtan Leonardo Antonio Sechi Samin Zamani Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patients Heliyon Mycobacterium avium subspecies paratuberculosis Multiple sclerosis Interferon regulatory factor 5 protein Human ELISA Nested PCR |
| title | Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patients |
| title_full | Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patients |
| title_fullStr | Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patients |
| title_full_unstemmed | Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patients |
| title_short | Cross-reactivity between Mycobacterium avium subspecies paratuberculosis 4027 peptide and Human IRF5 may contribute to Multiple Sclerosis in Iranian patients |
| title_sort | cross reactivity between mycobacterium avium subspecies paratuberculosis 4027 peptide and human irf5 may contribute to multiple sclerosis in iranian patients |
| topic | Mycobacterium avium subspecies paratuberculosis Multiple sclerosis Interferon regulatory factor 5 protein Human ELISA Nested PCR |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023093453 |
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