Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper
To determine the mutagen agent potency and deduce an optimum dose, cytological analysis for induced chromosomal aberrations is considered as an accurate index in mutation breeding. Therefore, the present investigation was carried out to estimate the relative frequency and spectrum of meiotic abnorma...
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2019-09-01
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| Series: | Biotecnología Vegetal |
| Subjects: | |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/633 |
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| author | Sonu Goyal Mohammad Rafiq Wani Rafiul Amin Laskar Aamir Raina Samiullah Khan |
| author_facet | Sonu Goyal Mohammad Rafiq Wani Rafiul Amin Laskar Aamir Raina Samiullah Khan |
| author_sort | Sonu Goyal |
| collection | DOAJ |
| description | To determine the mutagen agent potency and deduce an optimum dose, cytological analysis for induced chromosomal aberrations is considered as an accurate index in mutation breeding. Therefore, the present investigation was carried out to estimate the relative frequency and spectrum of meiotic abnormalities at various stages of cell division using Gamma rays, EMS and their combination treatments in M1 generation of black gram (Vigna mungo L. Hepper) varieties Pant U-30 and T-9. The analysis of M1 plants revealed a wide range of induced meiotic abnormalities like univalents, multivalents, laggards, chromatin bridges, micronuclei and chromosome stickiness by different mutagen doses. In addition to these, behavioral changes like precocious separation of chromosomes at metaphase, unequal separation of bivalents at anaphase, disturbed polarity and cytomixis at telophase were observed at low frequency. A linear relationship between the frequency of chromosomal aberrations and the strength of mutagen doses was observed in both the varieties and mutagen types. It was found that the combined treatments induced meiotic abnormalities at a higher frequency as compared to individual treatments of Gamma rays and EMS. Comparative estimation of induced cytological abnormalities suggested higher mutagenic sensitivity of var. Pant U-30 than the var. T-9 towards the treatments used. This also confirmed the relatively broader genetic tolerance level of black gram var. T-9 in inducing a significant amount of viable mutations without much lethality at the mutagen doses recommended in this study. Therefore, it can be concluded that the combine treatments of Gamma rays and EMS (300Gy+0.2%, 200Gy+0.3%) are relatively more effective for induction mutations in var. T-9 at the genotypic tolerable level. |
| first_indexed | 2024-12-11T01:58:09Z |
| format | Article |
| id | doaj.art-2e09f89f6e574ee794b70b7cea26077b |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-11T01:58:09Z |
| publishDate | 2019-09-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-2e09f89f6e574ee794b70b7cea26077b2022-12-22T01:24:33ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472019-09-01193193204Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper Sonu Goyal0 Mohammad Rafiq Wani1Rafiul Amin Laskar2Aamir Raina3Samiullah Khan4 Instituto de Biotecnología de las Plantas. Universidad Central "Marta Abreu" de Las Villas; India Department of Botany, Abdul Ahad Azad Memorial Degree College. Bemina-190 018 Cluster University. Srinagar. Jammu and Kashmir. India.; India Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University. Aligarh-202 002. Aligarh. Uttar Pradesh. India.; India Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University. Aligarh-202 002. Aligarh. Uttar Pradesh. India. Botany Section, Women’s College, Aligarh Muslim University. Aligarh-202 002. Aligarh. Uttar Pradesh. India.; India Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University. Aligarh-202 002. Aligarh. Uttar Pradesh. India.; India To determine the mutagen agent potency and deduce an optimum dose, cytological analysis for induced chromosomal aberrations is considered as an accurate index in mutation breeding. Therefore, the present investigation was carried out to estimate the relative frequency and spectrum of meiotic abnormalities at various stages of cell division using Gamma rays, EMS and their combination treatments in M1 generation of black gram (Vigna mungo L. Hepper) varieties Pant U-30 and T-9. The analysis of M1 plants revealed a wide range of induced meiotic abnormalities like univalents, multivalents, laggards, chromatin bridges, micronuclei and chromosome stickiness by different mutagen doses. In addition to these, behavioral changes like precocious separation of chromosomes at metaphase, unequal separation of bivalents at anaphase, disturbed polarity and cytomixis at telophase were observed at low frequency. A linear relationship between the frequency of chromosomal aberrations and the strength of mutagen doses was observed in both the varieties and mutagen types. It was found that the combined treatments induced meiotic abnormalities at a higher frequency as compared to individual treatments of Gamma rays and EMS. Comparative estimation of induced cytological abnormalities suggested higher mutagenic sensitivity of var. Pant U-30 than the var. T-9 towards the treatments used. This also confirmed the relatively broader genetic tolerance level of black gram var. T-9 in inducing a significant amount of viable mutations without much lethality at the mutagen doses recommended in this study. Therefore, it can be concluded that the combine treatments of Gamma rays and EMS (300Gy+0.2%, 200Gy+0.3%) are relatively more effective for induction mutations in var. T-9 at the genotypic tolerable level.https://revista.ibp.co.cu/index.php/BV/article/view/633genotypic sensitivityinduced mutagenesismeiotic aberration frequencymutation breedingpollen mother cells |
| spellingShingle | Sonu Goyal Mohammad Rafiq Wani Rafiul Amin Laskar Aamir Raina Samiullah Khan Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper Biotecnología Vegetal genotypic sensitivity induced mutagenesis meiotic aberration frequency mutation breeding pollen mother cells |
| title | Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper |
| title_full | Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper |
| title_fullStr | Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper |
| title_full_unstemmed | Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper |
| title_short | Assessment on cytotoxic and mutagenic potency of Gamma rays and EMS in Vigna mungo L. Hepper |
| title_sort | assessment on cytotoxic and mutagenic potency of gamma rays and ems in vigna mungo l hepper |
| topic | genotypic sensitivity induced mutagenesis meiotic aberration frequency mutation breeding pollen mother cells |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/633 |
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