Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia
Preeclampsia (PE) is a serious disease during pregnancy associated with the dysfunction of trophoblast cell invasion. DDX46 is a kind of RNA helicase that has been found to regulate cancer cell metastasis. However, the role of DDX46 in PE remains unclear. Our results showed that the mRNA levels of D...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2020-06-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2020-0043 |
| _version_ | 1819016136774123520 |
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| author | You Xin Cui Hongyan Yu Ning Li Qiuli |
| author_facet | You Xin Cui Hongyan Yu Ning Li Qiuli |
| author_sort | You Xin |
| collection | DOAJ |
| description | Preeclampsia (PE) is a serious disease during pregnancy associated with the dysfunction of trophoblast cell invasion. DDX46 is a kind of RNA helicase that has been found to regulate cancer cell metastasis. However, the role of DDX46 in PE remains unclear. Our results showed that the mRNA levels of DDX46 in placental tissues of pregnant women with PE were markedly lower than those in normal pregnancies. Loss-of-function assays showed that knockdown of DDX46 significantly suppressed cell proliferation of trophoblast cells. Besides, DDX46 knockdown decreased trophoblast cell migration and invasion capacity. In contrast, the overexpression of DDX46 promoted the migration and invasion of trophoblast cells. Furthermore, knockdown of DDX46 caused significant decrease in the levels of p-PI3K, p-Akt, and p-mTOR in HTR-8/SVneo cells. In addition, treatment with IGF-1 reversed the inhibitory effects of DDX46 knockdown on proliferation, migration, and invasion of HTR-8/SVneo cells. In conclusion, these data suggest that DDX46 might be involved in the progression of PE, which might be attributed to the regulation of PI3K/Akt/mTOR signaling pathway. Thus, DDX46 might serve as a therapeutic target for the treatment of PE. |
| first_indexed | 2024-12-21T02:42:50Z |
| format | Article |
| id | doaj.art-2e0d6aa93e7341cc836e6d73ab5c2a9a |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-21T02:42:50Z |
| publishDate | 2020-06-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-2e0d6aa93e7341cc836e6d73ab5c2a9a2022-12-21T19:18:39ZengDe GruyterOpen Life Sciences2391-54122020-06-0115140040810.1515/biol-2020-0043biol-2020-0043Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsiaYou Xin0Cui Hongyan1Yu Ning2Li Qiuli3Department of Obstetrics, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300052, ChinaDepartment of Obstetrics, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300052, ChinaDepartment of Obstetrics, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300052, ChinaDepartment of Laboratory, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300052, ChinaPreeclampsia (PE) is a serious disease during pregnancy associated with the dysfunction of trophoblast cell invasion. DDX46 is a kind of RNA helicase that has been found to regulate cancer cell metastasis. However, the role of DDX46 in PE remains unclear. Our results showed that the mRNA levels of DDX46 in placental tissues of pregnant women with PE were markedly lower than those in normal pregnancies. Loss-of-function assays showed that knockdown of DDX46 significantly suppressed cell proliferation of trophoblast cells. Besides, DDX46 knockdown decreased trophoblast cell migration and invasion capacity. In contrast, the overexpression of DDX46 promoted the migration and invasion of trophoblast cells. Furthermore, knockdown of DDX46 caused significant decrease in the levels of p-PI3K, p-Akt, and p-mTOR in HTR-8/SVneo cells. In addition, treatment with IGF-1 reversed the inhibitory effects of DDX46 knockdown on proliferation, migration, and invasion of HTR-8/SVneo cells. In conclusion, these data suggest that DDX46 might be involved in the progression of PE, which might be attributed to the regulation of PI3K/Akt/mTOR signaling pathway. Thus, DDX46 might serve as a therapeutic target for the treatment of PE.https://doi.org/10.1515/biol-2020-0043preeclampsiatrophoblast cellsrna helicasesddx46pi3k/akt/mtor pathway |
| spellingShingle | You Xin Cui Hongyan Yu Ning Li Qiuli Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia Open Life Sciences preeclampsia trophoblast cells rna helicases ddx46 pi3k/akt/mtor pathway |
| title | Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia |
| title_full | Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia |
| title_fullStr | Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia |
| title_full_unstemmed | Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia |
| title_short | Knockdown of DDX46 inhibits trophoblast cell proliferation and migration through the PI3K/Akt/mTOR signaling pathway in preeclampsia |
| title_sort | knockdown of ddx46 inhibits trophoblast cell proliferation and migration through the pi3k akt mtor signaling pathway in preeclampsia |
| topic | preeclampsia trophoblast cells rna helicases ddx46 pi3k/akt/mtor pathway |
| url | https://doi.org/10.1515/biol-2020-0043 |
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