Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity
There is no standardized protocol for the assessment of microbial air contamination in museums and other cultural heritage sites. Therefore, most museums conduct such assessments based on their own guidelines or good practices. Usually, microbial air contamination is assessed using only classical mi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/10/20/7128 |
| _version_ | 1827704408940478464 |
|---|---|
| author | Mikolaj Dziurzynski Karol Ciuchcinski Magdalena Dyda Anna Szych Paulina Drabik Agnieszka Laudy Lukasz Dziewit |
| author_facet | Mikolaj Dziurzynski Karol Ciuchcinski Magdalena Dyda Anna Szych Paulina Drabik Agnieszka Laudy Lukasz Dziewit |
| author_sort | Mikolaj Dziurzynski |
| collection | DOAJ |
| description | There is no standardized protocol for the assessment of microbial air contamination in museums and other cultural heritage sites. Therefore, most museums conduct such assessments based on their own guidelines or good practices. Usually, microbial air contamination is assessed using only classical microbiology methods with the application of a single growth medium. Therefore, this medium should be carefully selected to limit any selective cultivation bias. Metabarcoding, i.e., a next-generation sequencing (NGS)-based method, combined with classical microbiological culturing was used to assess the effectiveness of various media applications in microbiological screening at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland). The obtained results indicated that when using a classical microbiology approach to assess the microbial air contamination at the museum, the selection of a proper growth medium was critical. It was shown that the use of rich media (commonly applied by museum conservators) introduced significant bias by severely underreporting putative human pathogens and the bacterial species involved in biodeterioration. Therefore, we recommend the use of other media, such as Frazier or Reasoner’s 2A (R2A) medium, as they could yield more diverse communities and recovered the highest number of genera containing human pathogens, which may be suitable for public health assessments. |
| first_indexed | 2024-03-10T15:39:16Z |
| format | Article |
| id | doaj.art-2e0f1b0394ab4a27a6a52e5b478f4705 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T15:39:16Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-2e0f1b0394ab4a27a6a52e5b478f47052023-11-20T16:56:19ZengMDPI AGApplied Sciences2076-34172020-10-011020712810.3390/app10207128Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria DiversityMikolaj Dziurzynski0Karol Ciuchcinski1Magdalena Dyda2Anna Szych3Paulina Drabik4Agnieszka Laudy5Lukasz Dziewit6Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandResearch and Development for Life Sciences Ltd., Miecznikowa 1/5a, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandResearch and Development for Life Sciences Ltd., Miecznikowa 1/5a, 02-096 Warsaw, PolandMuseum of King John III’s Palace at Wilanow, Laboratory of Environmental Analysis, Stanislawa Kostki Potockiego 10/16, 02-958 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandThere is no standardized protocol for the assessment of microbial air contamination in museums and other cultural heritage sites. Therefore, most museums conduct such assessments based on their own guidelines or good practices. Usually, microbial air contamination is assessed using only classical microbiology methods with the application of a single growth medium. Therefore, this medium should be carefully selected to limit any selective cultivation bias. Metabarcoding, i.e., a next-generation sequencing (NGS)-based method, combined with classical microbiological culturing was used to assess the effectiveness of various media applications in microbiological screening at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland). The obtained results indicated that when using a classical microbiology approach to assess the microbial air contamination at the museum, the selection of a proper growth medium was critical. It was shown that the use of rich media (commonly applied by museum conservators) introduced significant bias by severely underreporting putative human pathogens and the bacterial species involved in biodeterioration. Therefore, we recommend the use of other media, such as Frazier or Reasoner’s 2A (R2A) medium, as they could yield more diverse communities and recovered the highest number of genera containing human pathogens, which may be suitable for public health assessments.https://www.mdpi.com/2076-3417/10/20/7128air qualitycultural heritagemuseumnext-generation sequencingPCR amplicon |
| spellingShingle | Mikolaj Dziurzynski Karol Ciuchcinski Magdalena Dyda Anna Szych Paulina Drabik Agnieszka Laudy Lukasz Dziewit Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity Applied Sciences air quality cultural heritage museum next-generation sequencing PCR amplicon |
| title | Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity |
| title_full | Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity |
| title_fullStr | Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity |
| title_full_unstemmed | Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity |
| title_short | Assessment of Bacterial Contamination of Air at the Museum of King John III’s Palace at Wilanow (Warsaw, Poland): Selection of an Optimal Growth Medium for Analyzing Airborne Bacteria Diversity |
| title_sort | assessment of bacterial contamination of air at the museum of king john iii s palace at wilanow warsaw poland selection of an optimal growth medium for analyzing airborne bacteria diversity |
| topic | air quality cultural heritage museum next-generation sequencing PCR amplicon |
| url | https://www.mdpi.com/2076-3417/10/20/7128 |
| work_keys_str_mv | AT mikolajdziurzynski assessmentofbacterialcontaminationofairatthemuseumofkingjohniiispalaceatwilanowwarsawpolandselectionofanoptimalgrowthmediumforanalyzingairbornebacteriadiversity AT karolciuchcinski assessmentofbacterialcontaminationofairatthemuseumofkingjohniiispalaceatwilanowwarsawpolandselectionofanoptimalgrowthmediumforanalyzingairbornebacteriadiversity AT magdalenadyda assessmentofbacterialcontaminationofairatthemuseumofkingjohniiispalaceatwilanowwarsawpolandselectionofanoptimalgrowthmediumforanalyzingairbornebacteriadiversity AT annaszych assessmentofbacterialcontaminationofairatthemuseumofkingjohniiispalaceatwilanowwarsawpolandselectionofanoptimalgrowthmediumforanalyzingairbornebacteriadiversity AT paulinadrabik assessmentofbacterialcontaminationofairatthemuseumofkingjohniiispalaceatwilanowwarsawpolandselectionofanoptimalgrowthmediumforanalyzingairbornebacteriadiversity AT agnieszkalaudy assessmentofbacterialcontaminationofairatthemuseumofkingjohniiispalaceatwilanowwarsawpolandselectionofanoptimalgrowthmediumforanalyzingairbornebacteriadiversity AT lukaszdziewit assessmentofbacterialcontaminationofairatthemuseumofkingjohniiispalaceatwilanowwarsawpolandselectionofanoptimalgrowthmediumforanalyzingairbornebacteriadiversity |