Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis
Oxyresveratrol (ORes, trans-2,4,3′,5′-tetrahydroxy stilbene) naturally exists in mulberry, grapes, peanuts and other plants. It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol (Res). Hence, ORes has stronger antioxidant activity than r...
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| Format: | Article |
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Tsinghua University Press
2021-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301920 |
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| author | Guliang Yang Jianfeng Zhan Yiwen Yang Li Yuan Peilei Wang Chi-Tang Ho Shiming Li |
| author_facet | Guliang Yang Jianfeng Zhan Yiwen Yang Li Yuan Peilei Wang Chi-Tang Ho Shiming Li |
| author_sort | Guliang Yang |
| collection | DOAJ |
| description | Oxyresveratrol (ORes, trans-2,4,3′,5′-tetrahydroxy stilbene) naturally exists in mulberry, grapes, peanuts and other plants. It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol (Res). Hence, ORes has stronger antioxidant activity than resveratrol. In present study, we employed a rat hepatic fibrosis model induced by carbon tetrachloride (CCl4) and administrated ORes via gavage feeding to study the protective effects and potential mechanisms of ORes against hepatic fibrosis. We demonstrated that rat liver oxidative damage induced by CCl4 was significantly alleviated after ORes feeding. Furthermore, the mRNA transcription levels of α-smooth muscle actinn (α-SMA), desmin, and two MMPs (MMP2 and MMP9) were reduced and the expression levels of transforming growth factor β1 (TGF-β1), p- small mother against decapen-taplegic protein (Smad)1/2 and p-extracellular signal-regulated kinases (ERK)1/2 in the liver tissue down-regulated dramatically. In a parallel study with Res, ORes showed more efficacious protective effect than Res against rat liver fibrosis, which is attributed to extended conjugation system due to the extra hydroxyl group at 2-position on ORes making it more electron-rich and susceptible to oxidation than Res. Therefore, dietary consumption of mulberry and other fruits containing ORes may be beneficial in the prevention of liver fibrosis. |
| first_indexed | 2024-03-12T06:32:46Z |
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| id | doaj.art-2e1af60ffae14c7f9bc24a8f4e126378 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T06:32:46Z |
| publishDate | 2021-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-2e1af60ffae14c7f9bc24a8f4e1263782023-09-03T01:32:26ZengTsinghua University PressFood Science and Human Wellness2213-45302021-01-01101612Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosisGuliang Yang0Jianfeng Zhan1Yiwen Yang2Li Yuan3Peilei Wang4Chi-Tang Ho5Shiming Li6National Engineering Laboratory for Rice and By-Products Processing, Food Science and Engineering College, Central South University of Forestry and Technology, Changsha, Hunan 410004, ChinaHubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, China; Corresponding authors at: Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, China.Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, ChinaHubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, ChinaHubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, ChinaDepartment of Food Science, Rutgers University, New Brunswick, NJ, USAHubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, China; Department of Food Science, Rutgers University, New Brunswick, NJ, USA; Corresponding authors at: Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei, China.Oxyresveratrol (ORes, trans-2,4,3′,5′-tetrahydroxy stilbene) naturally exists in mulberry, grapes, peanuts and other plants. It belongs to stilbene polyphenolic family and has an extra hydroxyl group at 2-position comparing with resveratrol (Res). Hence, ORes has stronger antioxidant activity than resveratrol. In present study, we employed a rat hepatic fibrosis model induced by carbon tetrachloride (CCl4) and administrated ORes via gavage feeding to study the protective effects and potential mechanisms of ORes against hepatic fibrosis. We demonstrated that rat liver oxidative damage induced by CCl4 was significantly alleviated after ORes feeding. Furthermore, the mRNA transcription levels of α-smooth muscle actinn (α-SMA), desmin, and two MMPs (MMP2 and MMP9) were reduced and the expression levels of transforming growth factor β1 (TGF-β1), p- small mother against decapen-taplegic protein (Smad)1/2 and p-extracellular signal-regulated kinases (ERK)1/2 in the liver tissue down-regulated dramatically. In a parallel study with Res, ORes showed more efficacious protective effect than Res against rat liver fibrosis, which is attributed to extended conjugation system due to the extra hydroxyl group at 2-position on ORes making it more electron-rich and susceptible to oxidation than Res. Therefore, dietary consumption of mulberry and other fruits containing ORes may be beneficial in the prevention of liver fibrosis.http://www.sciencedirect.com/science/article/pii/S2213453020301920OxyresveratrolHepatic fibrosisOxidative stressTGF-β/Smad/ERK signaling pathway |
| spellingShingle | Guliang Yang Jianfeng Zhan Yiwen Yang Li Yuan Peilei Wang Chi-Tang Ho Shiming Li Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis Food Science and Human Wellness Oxyresveratrol Hepatic fibrosis Oxidative stress TGF-β/Smad/ERK signaling pathway |
| title | Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis |
| title_full | Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis |
| title_fullStr | Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis |
| title_full_unstemmed | Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis |
| title_short | Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis |
| title_sort | inhibitory effects of oxyresveratrol on erk and smad1 2 phosphorylation and hsc activation in preventing carbon tetrachloride induced rat liver fibrosis |
| topic | Oxyresveratrol Hepatic fibrosis Oxidative stress TGF-β/Smad/ERK signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301920 |
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