Characterization of CTX-M-14-producing Escherichia coli from food-producing animals

Bacterial resistance to the third-generation cephalosporin antibiotics has become a major concern for public health. This study was aimed to determine the characteristics and distribution of blaCTX-M-14, which encodes an extended-spectrum β-lactamase, in E. coli isolated from Guangdong Province, Chi...

Full description

Bibliographic Details
Main Authors: Xiao-Ping eLiao, Jing eXia, Lei eYang, Liang eLi, Jian eSun, Ya-Hong eLiu, Hong-Xia eJiang
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-10-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01136/full
_version_ 1818017962131456000
author Xiao-Ping eLiao
Jing eXia
Lei eYang
Liang eLi
Jian eSun
Ya-Hong eLiu
Ya-Hong eLiu
Hong-Xia eJiang
author_facet Xiao-Ping eLiao
Jing eXia
Lei eYang
Liang eLi
Jian eSun
Ya-Hong eLiu
Ya-Hong eLiu
Hong-Xia eJiang
author_sort Xiao-Ping eLiao
collection DOAJ
description Bacterial resistance to the third-generation cephalosporin antibiotics has become a major concern for public health. This study was aimed to determine the characteristics and distribution of blaCTX-M-14, which encodes an extended-spectrum β-lactamase, in E. coli isolated from Guangdong Province, China. A total of 979 E. coli isolates isolated from healthy or diseased food-producing animals including swine and avian were examined for blaCTX-M-14 and then the blaCTX-M-14 –positive isolates were detected by other resistance determinants (ESBLs, PMQR, rmtB and floR) and analyzed by phylogenetic grouping analysis, PCR-based plasmid replicon typing, multilocus sequence typing and plasmid analysis. The genetic environments of blaCTX-M-14 were also determined by PCR. The results showed that fourteen CTX-M-14-producing E. coli were identified, belonging to groups A (7/14), B1 (4/14) and D (3/14). The most predominant resistance gene was blaTEM (n= 8), followed by floR (n=7), oqxA (n=3), aac(6’)-1b-cr (n=2) and rmtB (n=1). Plasmids carrying blaCTX-M-14 were classified to IncK, IncHI2, IncHI1, IncN, IncFIB, IncF or IncI1, ranged from about 30kb to 200kb, and with insertion sequence of ISEcp1, IS26 or ORF513 located upstream and IS903 downstream of blaCTX-M-14. The result of MLST showed that 14 isolates had 11 STs, and the 11 STs belonged to 5 groups. Many of the identified STs are reported to be common in E. coli isolates associated with extraintestinal infections in humans, suggesting possible transmission of blaCTX-M-14 between animals and humans. The difference in the flanking sequences of blaCTX-M-14 between the 2009 isolates and the early ones suggests that the resistance gene context continues to evolve in E. coli of food producing animals.
first_indexed 2024-04-14T07:33:38Z
format Article
id doaj.art-2e23b62cc8f74a70829fc8733c519981
institution Directory Open Access Journal
issn 1664-302X
language English
last_indexed 2024-04-14T07:33:38Z
publishDate 2015-10-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Microbiology
spelling doaj.art-2e23b62cc8f74a70829fc8733c5199812022-12-22T02:05:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-10-01610.3389/fmicb.2015.01136134106Characterization of CTX-M-14-producing Escherichia coli from food-producing animalsXiao-Ping eLiao0Jing eXia1Lei eYang2Liang eLi3Jian eSun4Ya-Hong eLiu5Ya-Hong eLiu6Hong-Xia eJiang7South China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversityJiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and ZoonosesSouth China Agricultural UniversityBacterial resistance to the third-generation cephalosporin antibiotics has become a major concern for public health. This study was aimed to determine the characteristics and distribution of blaCTX-M-14, which encodes an extended-spectrum β-lactamase, in E. coli isolated from Guangdong Province, China. A total of 979 E. coli isolates isolated from healthy or diseased food-producing animals including swine and avian were examined for blaCTX-M-14 and then the blaCTX-M-14 –positive isolates were detected by other resistance determinants (ESBLs, PMQR, rmtB and floR) and analyzed by phylogenetic grouping analysis, PCR-based plasmid replicon typing, multilocus sequence typing and plasmid analysis. The genetic environments of blaCTX-M-14 were also determined by PCR. The results showed that fourteen CTX-M-14-producing E. coli were identified, belonging to groups A (7/14), B1 (4/14) and D (3/14). The most predominant resistance gene was blaTEM (n= 8), followed by floR (n=7), oqxA (n=3), aac(6’)-1b-cr (n=2) and rmtB (n=1). Plasmids carrying blaCTX-M-14 were classified to IncK, IncHI2, IncHI1, IncN, IncFIB, IncF or IncI1, ranged from about 30kb to 200kb, and with insertion sequence of ISEcp1, IS26 or ORF513 located upstream and IS903 downstream of blaCTX-M-14. The result of MLST showed that 14 isolates had 11 STs, and the 11 STs belonged to 5 groups. Many of the identified STs are reported to be common in E. coli isolates associated with extraintestinal infections in humans, suggesting possible transmission of blaCTX-M-14 between animals and humans. The difference in the flanking sequences of blaCTX-M-14 between the 2009 isolates and the early ones suggests that the resistance gene context continues to evolve in E. coli of food producing animals.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01136/fullEscherichia coliPlasmidsmlstCTX-M-14cephalosporin
spellingShingle Xiao-Ping eLiao
Jing eXia
Lei eYang
Liang eLi
Jian eSun
Ya-Hong eLiu
Ya-Hong eLiu
Hong-Xia eJiang
Characterization of CTX-M-14-producing Escherichia coli from food-producing animals
Frontiers in Microbiology
Escherichia coli
Plasmids
mlst
CTX-M-14
cephalosporin
title Characterization of CTX-M-14-producing Escherichia coli from food-producing animals
title_full Characterization of CTX-M-14-producing Escherichia coli from food-producing animals
title_fullStr Characterization of CTX-M-14-producing Escherichia coli from food-producing animals
title_full_unstemmed Characterization of CTX-M-14-producing Escherichia coli from food-producing animals
title_short Characterization of CTX-M-14-producing Escherichia coli from food-producing animals
title_sort characterization of ctx m 14 producing escherichia coli from food producing animals
topic Escherichia coli
Plasmids
mlst
CTX-M-14
cephalosporin
url http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01136/full
work_keys_str_mv AT xiaopingeliao characterizationofctxm14producingescherichiacolifromfoodproducinganimals
AT jingexia characterizationofctxm14producingescherichiacolifromfoodproducinganimals
AT leieyang characterizationofctxm14producingescherichiacolifromfoodproducinganimals
AT liangeli characterizationofctxm14producingescherichiacolifromfoodproducinganimals
AT jianesun characterizationofctxm14producingescherichiacolifromfoodproducinganimals
AT yahongeliu characterizationofctxm14producingescherichiacolifromfoodproducinganimals
AT yahongeliu characterizationofctxm14producingescherichiacolifromfoodproducinganimals
AT hongxiaejiang characterizationofctxm14producingescherichiacolifromfoodproducinganimals