Characterization of CTX-M-14-producing Escherichia coli from food-producing animals
Bacterial resistance to the third-generation cephalosporin antibiotics has become a major concern for public health. This study was aimed to determine the characteristics and distribution of blaCTX-M-14, which encodes an extended-spectrum β-lactamase, in E. coli isolated from Guangdong Province, Chi...
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Language: | English |
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Frontiers Media S.A.
2015-10-01
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Series: | Frontiers in Microbiology |
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01136/full |
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author | Xiao-Ping eLiao Jing eXia Lei eYang Liang eLi Jian eSun Ya-Hong eLiu Ya-Hong eLiu Hong-Xia eJiang |
author_facet | Xiao-Ping eLiao Jing eXia Lei eYang Liang eLi Jian eSun Ya-Hong eLiu Ya-Hong eLiu Hong-Xia eJiang |
author_sort | Xiao-Ping eLiao |
collection | DOAJ |
description | Bacterial resistance to the third-generation cephalosporin antibiotics has become a major concern for public health. This study was aimed to determine the characteristics and distribution of blaCTX-M-14, which encodes an extended-spectrum β-lactamase, in E. coli isolated from Guangdong Province, China. A total of 979 E. coli isolates isolated from healthy or diseased food-producing animals including swine and avian were examined for blaCTX-M-14 and then the blaCTX-M-14 –positive isolates were detected by other resistance determinants (ESBLs, PMQR, rmtB and floR) and analyzed by phylogenetic grouping analysis, PCR-based plasmid replicon typing, multilocus sequence typing and plasmid analysis. The genetic environments of blaCTX-M-14 were also determined by PCR. The results showed that fourteen CTX-M-14-producing E. coli were identified, belonging to groups A (7/14), B1 (4/14) and D (3/14). The most predominant resistance gene was blaTEM (n= 8), followed by floR (n=7), oqxA (n=3), aac(6’)-1b-cr (n=2) and rmtB (n=1). Plasmids carrying blaCTX-M-14 were classified to IncK, IncHI2, IncHI1, IncN, IncFIB, IncF or IncI1, ranged from about 30kb to 200kb, and with insertion sequence of ISEcp1, IS26 or ORF513 located upstream and IS903 downstream of blaCTX-M-14. The result of MLST showed that 14 isolates had 11 STs, and the 11 STs belonged to 5 groups. Many of the identified STs are reported to be common in E. coli isolates associated with extraintestinal infections in humans, suggesting possible transmission of blaCTX-M-14 between animals and humans. The difference in the flanking sequences of blaCTX-M-14 between the 2009 isolates and the early ones suggests that the resistance gene context continues to evolve in E. coli of food producing animals. |
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institution | Directory Open Access Journal |
issn | 1664-302X |
language | English |
last_indexed | 2024-04-14T07:33:38Z |
publishDate | 2015-10-01 |
publisher | Frontiers Media S.A. |
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series | Frontiers in Microbiology |
spelling | doaj.art-2e23b62cc8f74a70829fc8733c5199812022-12-22T02:05:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-10-01610.3389/fmicb.2015.01136134106Characterization of CTX-M-14-producing Escherichia coli from food-producing animalsXiao-Ping eLiao0Jing eXia1Lei eYang2Liang eLi3Jian eSun4Ya-Hong eLiu5Ya-Hong eLiu6Hong-Xia eJiang7South China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversitySouth China Agricultural UniversityJiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and ZoonosesSouth China Agricultural UniversityBacterial resistance to the third-generation cephalosporin antibiotics has become a major concern for public health. This study was aimed to determine the characteristics and distribution of blaCTX-M-14, which encodes an extended-spectrum β-lactamase, in E. coli isolated from Guangdong Province, China. A total of 979 E. coli isolates isolated from healthy or diseased food-producing animals including swine and avian were examined for blaCTX-M-14 and then the blaCTX-M-14 –positive isolates were detected by other resistance determinants (ESBLs, PMQR, rmtB and floR) and analyzed by phylogenetic grouping analysis, PCR-based plasmid replicon typing, multilocus sequence typing and plasmid analysis. The genetic environments of blaCTX-M-14 were also determined by PCR. The results showed that fourteen CTX-M-14-producing E. coli were identified, belonging to groups A (7/14), B1 (4/14) and D (3/14). The most predominant resistance gene was blaTEM (n= 8), followed by floR (n=7), oqxA (n=3), aac(6’)-1b-cr (n=2) and rmtB (n=1). Plasmids carrying blaCTX-M-14 were classified to IncK, IncHI2, IncHI1, IncN, IncFIB, IncF or IncI1, ranged from about 30kb to 200kb, and with insertion sequence of ISEcp1, IS26 or ORF513 located upstream and IS903 downstream of blaCTX-M-14. The result of MLST showed that 14 isolates had 11 STs, and the 11 STs belonged to 5 groups. Many of the identified STs are reported to be common in E. coli isolates associated with extraintestinal infections in humans, suggesting possible transmission of blaCTX-M-14 between animals and humans. The difference in the flanking sequences of blaCTX-M-14 between the 2009 isolates and the early ones suggests that the resistance gene context continues to evolve in E. coli of food producing animals.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01136/fullEscherichia coliPlasmidsmlstCTX-M-14cephalosporin |
spellingShingle | Xiao-Ping eLiao Jing eXia Lei eYang Liang eLi Jian eSun Ya-Hong eLiu Ya-Hong eLiu Hong-Xia eJiang Characterization of CTX-M-14-producing Escherichia coli from food-producing animals Frontiers in Microbiology Escherichia coli Plasmids mlst CTX-M-14 cephalosporin |
title | Characterization of CTX-M-14-producing Escherichia coli from food-producing animals |
title_full | Characterization of CTX-M-14-producing Escherichia coli from food-producing animals |
title_fullStr | Characterization of CTX-M-14-producing Escherichia coli from food-producing animals |
title_full_unstemmed | Characterization of CTX-M-14-producing Escherichia coli from food-producing animals |
title_short | Characterization of CTX-M-14-producing Escherichia coli from food-producing animals |
title_sort | characterization of ctx m 14 producing escherichia coli from food producing animals |
topic | Escherichia coli Plasmids mlst CTX-M-14 cephalosporin |
url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01136/full |
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