Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice

The contribution deals with thermal stability and re-solubility of potato tuber proteins isolated by ethanol precipitation from industrial potato fruit juice. The protein isolate was exposed to the temperatures ranging from 25°C to 70°C. Patatin, the tuber protein with a high nutritious value, was d...

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Main Authors: V. Bártová, J. Bárta
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2008-12-01
Series:Research in Agricultural Engineering
Subjects:
Online Access:https://rae.agriculturejournals.cz/artkey/rae-200804-0003_effect-of-heat-treatment-on-re-solubility-of-potato-proteins-isolated-from-industrial-potato-fruit-juice.php
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author V. Bártová
J. Bárta
author_facet V. Bártová
J. Bárta
author_sort V. Bártová
collection DOAJ
description The contribution deals with thermal stability and re-solubility of potato tuber proteins isolated by ethanol precipitation from industrial potato fruit juice. The protein isolate was exposed to the temperatures ranging from 25°C to 70°C. Patatin, the tuber protein with a high nutritious value, was detected by SDS-PAGE in the region of 39-43 kDa. Patatin was evaluated as thermal sensitive - temperatures above 30°C caused its strong insolubility. Potato protease inhibitors were detected in the region from 4.3 to 24 kDa. Thermal stability of potato protease inhibitors (region from 25 to 14 kDa) was higher, although the temperatures above 45°C caused denaturation and insolubility of most of the protease inhibitors. Extremely thermo-stable was potato carboxypeptidase inhibitor with molecular weight of 4.3 kDa that remained soluble even after having been exposed to the highest temperatures.
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spelling doaj.art-2e268526517b4359b2b0379e4f6de60b2023-02-23T03:47:20ZengCzech Academy of Agricultural SciencesResearch in Agricultural Engineering1212-91511805-93762008-12-0154417017510.17221/703-RAErae-200804-0003Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juiceV. Bártová0J. Bárta1Biotechnological centre, Department of Plant Production, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech RepublicBiotechnological centre, Department of Plant Production, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech RepublicThe contribution deals with thermal stability and re-solubility of potato tuber proteins isolated by ethanol precipitation from industrial potato fruit juice. The protein isolate was exposed to the temperatures ranging from 25°C to 70°C. Patatin, the tuber protein with a high nutritious value, was detected by SDS-PAGE in the region of 39-43 kDa. Patatin was evaluated as thermal sensitive - temperatures above 30°C caused its strong insolubility. Potato protease inhibitors were detected in the region from 4.3 to 24 kDa. Thermal stability of potato protease inhibitors (region from 25 to 14 kDa) was higher, although the temperatures above 45°C caused denaturation and insolubility of most of the protease inhibitors. Extremely thermo-stable was potato carboxypeptidase inhibitor with molecular weight of 4.3 kDa that remained soluble even after having been exposed to the highest temperatures.https://rae.agriculturejournals.cz/artkey/rae-200804-0003_effect-of-heat-treatment-on-re-solubility-of-potato-proteins-isolated-from-industrial-potato-fruit-juice.phppotato proteins thermo-stabilitypotato fruit waterpatatinprotease inhibitors
spellingShingle V. Bártová
J. Bárta
Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice
Research in Agricultural Engineering
potato proteins thermo-stability
potato fruit water
patatin
protease inhibitors
title Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice
title_full Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice
title_fullStr Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice
title_full_unstemmed Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice
title_short Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice
title_sort effect of heat treatment on re solubility of potato proteins isolated from industrial potato fruit juice
topic potato proteins thermo-stability
potato fruit water
patatin
protease inhibitors
url https://rae.agriculturejournals.cz/artkey/rae-200804-0003_effect-of-heat-treatment-on-re-solubility-of-potato-proteins-isolated-from-industrial-potato-fruit-juice.php
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