Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice
The contribution deals with thermal stability and re-solubility of potato tuber proteins isolated by ethanol precipitation from industrial potato fruit juice. The protein isolate was exposed to the temperatures ranging from 25°C to 70°C. Patatin, the tuber protein with a high nutritious value, was d...
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Format: | Article |
Language: | English |
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Czech Academy of Agricultural Sciences
2008-12-01
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Series: | Research in Agricultural Engineering |
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Online Access: | https://rae.agriculturejournals.cz/artkey/rae-200804-0003_effect-of-heat-treatment-on-re-solubility-of-potato-proteins-isolated-from-industrial-potato-fruit-juice.php |
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author | V. Bártová J. Bárta |
author_facet | V. Bártová J. Bárta |
author_sort | V. Bártová |
collection | DOAJ |
description | The contribution deals with thermal stability and re-solubility of potato tuber proteins isolated by ethanol precipitation from industrial potato fruit juice. The protein isolate was exposed to the temperatures ranging from 25°C to 70°C. Patatin, the tuber protein with a high nutritious value, was detected by SDS-PAGE in the region of 39-43 kDa. Patatin was evaluated as thermal sensitive - temperatures above 30°C caused its strong insolubility. Potato protease inhibitors were detected in the region from 4.3 to 24 kDa. Thermal stability of potato protease inhibitors (region from 25 to 14 kDa) was higher, although the temperatures above 45°C caused denaturation and insolubility of most of the protease inhibitors. Extremely thermo-stable was potato carboxypeptidase inhibitor with molecular weight of 4.3 kDa that remained soluble even after having been exposed to the highest temperatures. |
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format | Article |
id | doaj.art-2e268526517b4359b2b0379e4f6de60b |
institution | Directory Open Access Journal |
issn | 1212-9151 1805-9376 |
language | English |
last_indexed | 2024-04-10T08:07:13Z |
publishDate | 2008-12-01 |
publisher | Czech Academy of Agricultural Sciences |
record_format | Article |
series | Research in Agricultural Engineering |
spelling | doaj.art-2e268526517b4359b2b0379e4f6de60b2023-02-23T03:47:20ZengCzech Academy of Agricultural SciencesResearch in Agricultural Engineering1212-91511805-93762008-12-0154417017510.17221/703-RAErae-200804-0003Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juiceV. Bártová0J. Bárta1Biotechnological centre, Department of Plant Production, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech RepublicBiotechnological centre, Department of Plant Production, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech RepublicThe contribution deals with thermal stability and re-solubility of potato tuber proteins isolated by ethanol precipitation from industrial potato fruit juice. The protein isolate was exposed to the temperatures ranging from 25°C to 70°C. Patatin, the tuber protein with a high nutritious value, was detected by SDS-PAGE in the region of 39-43 kDa. Patatin was evaluated as thermal sensitive - temperatures above 30°C caused its strong insolubility. Potato protease inhibitors were detected in the region from 4.3 to 24 kDa. Thermal stability of potato protease inhibitors (region from 25 to 14 kDa) was higher, although the temperatures above 45°C caused denaturation and insolubility of most of the protease inhibitors. Extremely thermo-stable was potato carboxypeptidase inhibitor with molecular weight of 4.3 kDa that remained soluble even after having been exposed to the highest temperatures.https://rae.agriculturejournals.cz/artkey/rae-200804-0003_effect-of-heat-treatment-on-re-solubility-of-potato-proteins-isolated-from-industrial-potato-fruit-juice.phppotato proteins thermo-stabilitypotato fruit waterpatatinprotease inhibitors |
spellingShingle | V. Bártová J. Bárta Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice Research in Agricultural Engineering potato proteins thermo-stability potato fruit water patatin protease inhibitors |
title | Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice |
title_full | Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice |
title_fullStr | Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice |
title_full_unstemmed | Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice |
title_short | Effect of heat treatment on re-solubility of potato proteins isolated from industrial potato fruit juice |
title_sort | effect of heat treatment on re solubility of potato proteins isolated from industrial potato fruit juice |
topic | potato proteins thermo-stability potato fruit water patatin protease inhibitors |
url | https://rae.agriculturejournals.cz/artkey/rae-200804-0003_effect-of-heat-treatment-on-re-solubility-of-potato-proteins-isolated-from-industrial-potato-fruit-juice.php |
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