Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches
Biofilms are assemblages of microbial cells, extracellular polymeric substances (EPS), and other components extracted from the environment in which they develop. Within biofilms, the spatial distribution of these components can vary. Here we present a fundamental characterization study to show diffe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/13/3890 |
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| author | Samuel Cheeseman Z. L. Shaw Jitraporn Vongsvivut Russell J. Crawford Madeleine F. Dupont Kylie J. Boyce Sheeana Gangadoo Saffron J. Bryant Gary Bryant Daniel Cozzolino James Chapman Aaron Elbourne Vi Khanh Truong |
| author_facet | Samuel Cheeseman Z. L. Shaw Jitraporn Vongsvivut Russell J. Crawford Madeleine F. Dupont Kylie J. Boyce Sheeana Gangadoo Saffron J. Bryant Gary Bryant Daniel Cozzolino James Chapman Aaron Elbourne Vi Khanh Truong |
| author_sort | Samuel Cheeseman |
| collection | DOAJ |
| description | Biofilms are assemblages of microbial cells, extracellular polymeric substances (EPS), and other components extracted from the environment in which they develop. Within biofilms, the spatial distribution of these components can vary. Here we present a fundamental characterization study to show differences between biofilms formed by Gram-positive methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), Gram-negative <i>Pseudomonas aeruginosa</i>, and the yeast-type <i>Candida albicans</i> using synchrotron macro attenuated total reflectance-Fourier transform infrared (ATR-FTIR) microspectroscopy. We were able to characterise the pathogenic biofilms’ heterogeneous distribution, which is challenging to do using traditional techniques. Multivariate analyses revealed that the polysaccharides area (1200–950 cm<sup>−1</sup>) accounted for the most significant variance between biofilm samples, and other spectral regions corresponding to amides, lipids, and polysaccharides all contributed to sample variation. In general, this study will advance our understanding of microbial biofilms and serve as a model for future research on how to use synchrotron source ATR-FTIR microspectroscopy to analyse their variations and spatial arrangements. |
| first_indexed | 2024-03-10T10:02:25Z |
| format | Article |
| id | doaj.art-2e26bd5ee97c4690b5317d058aff334b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:02:25Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-2e26bd5ee97c4690b5317d058aff334b2023-11-22T01:46:42ZengMDPI AGMolecules1420-30492021-06-012613389010.3390/molecules26133890Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric ApproachesSamuel Cheeseman0Z. L. Shaw1Jitraporn Vongsvivut2Russell J. Crawford3Madeleine F. Dupont4Kylie J. Boyce5Sheeana Gangadoo6Saffron J. Bryant7Gary Bryant8Daniel Cozzolino9James Chapman10Aaron Elbourne11Vi Khanh Truong12Nanobiotechnology Laboratory, School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaNanobiotechnology Laboratory, School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaInfrared Microspectroscopy Beamline, ANSTO Australian Synchrotron, Clayton, VIC 3168, AustraliaNanobiotechnology Laboratory, School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaNanobiotechnology Laboratory, School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaCentre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Brisbane, QLD 4072, AustraliaNanobiotechnology Laboratory, School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaNanobiotechnology Laboratory, School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaNanobiotechnology Laboratory, School of Science, College of Science, Engineering and Health, RMIT University, Melbourne, VIC 3001, AustraliaBiofilms are assemblages of microbial cells, extracellular polymeric substances (EPS), and other components extracted from the environment in which they develop. Within biofilms, the spatial distribution of these components can vary. Here we present a fundamental characterization study to show differences between biofilms formed by Gram-positive methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), Gram-negative <i>Pseudomonas aeruginosa</i>, and the yeast-type <i>Candida albicans</i> using synchrotron macro attenuated total reflectance-Fourier transform infrared (ATR-FTIR) microspectroscopy. We were able to characterise the pathogenic biofilms’ heterogeneous distribution, which is challenging to do using traditional techniques. Multivariate analyses revealed that the polysaccharides area (1200–950 cm<sup>−1</sup>) accounted for the most significant variance between biofilm samples, and other spectral regions corresponding to amides, lipids, and polysaccharides all contributed to sample variation. In general, this study will advance our understanding of microbial biofilms and serve as a model for future research on how to use synchrotron source ATR-FTIR microspectroscopy to analyse their variations and spatial arrangements.https://www.mdpi.com/1420-3049/26/13/3890biofilmssynchrotroninfraredchemometricsATRspatial heterogeneity |
| spellingShingle | Samuel Cheeseman Z. L. Shaw Jitraporn Vongsvivut Russell J. Crawford Madeleine F. Dupont Kylie J. Boyce Sheeana Gangadoo Saffron J. Bryant Gary Bryant Daniel Cozzolino James Chapman Aaron Elbourne Vi Khanh Truong Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches Molecules biofilms synchrotron infrared chemometrics ATR spatial heterogeneity |
| title | Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches |
| title_full | Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches |
| title_fullStr | Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches |
| title_full_unstemmed | Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches |
| title_short | Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches |
| title_sort | analysis of pathogenic bacterial and yeast biofilms using the combination of synchrotron atr ftir microspectroscopy and chemometric approaches |
| topic | biofilms synchrotron infrared chemometrics ATR spatial heterogeneity |
| url | https://www.mdpi.com/1420-3049/26/13/3890 |
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