Intraoperative Flow Cytometry for the Characterization of Gynecological Malignancies
Cell-cycle analysis has shown the presence of aneuploidy to be associated with poor prognosis. We developed an innovative rapid cell-cycle analysis protocol (the Ioannina protocol) that permitted the intraoperative identification of neoplastic cells in a plethora of malignancies. Herein, we aimed to...
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MDPI AG
2022-09-01
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| author | Zoi Anastasiadi Stefania Mantziou Christos Akrivis Minas Paschopoulos Eufemia Balasi Georgios D. Lianos George A. Alexiou Michail Mitsis George Vartholomatos Georgios S. Markopoulos |
| author_facet | Zoi Anastasiadi Stefania Mantziou Christos Akrivis Minas Paschopoulos Eufemia Balasi Georgios D. Lianos George A. Alexiou Michail Mitsis George Vartholomatos Georgios S. Markopoulos |
| author_sort | Zoi Anastasiadi |
| collection | DOAJ |
| description | Cell-cycle analysis has shown the presence of aneuploidy to be associated with poor prognosis. We developed an innovative rapid cell-cycle analysis protocol (the Ioannina protocol) that permitted the intraoperative identification of neoplastic cells in a plethora of malignancies. Herein, we aimed to investigate the potential role of cell-cycle analysis in the intraoperative characterization of gynecological malignancies. Women who underwent surgery for gynecological malignancies in our institution over a three-year period were included in this study. Permanent section pathology evaluation was used as the gold standard for malignancy evaluation. Total accordance was observed between flow cytometry and pathology evaluation. In total, 21 aneuploid cancers were detected following DNA index calculation. Of these, 20 were hyperploid and 1 was hypoploid. In addition, tumor samples were characterized by a significantly lower percentage of cells in G0/G1, as well as an induced tumor index. The response time for flow cytometry to obtain results was 5–6 min per sample. It seems that flow cytometry analyses for intraoperative tumor evaluation can be safely expanded to gynecological malignancies. This is a novel practical approach that has been proven valuable in several tumor types to date, and also seems to be reliable for gynecological malignancies. Intraoperative flow cytometry is expected to be crucial in decisions of lymph node dissection in endometrial cancers, due to its rapid response regarding the tumor invasion of part or all of the myometrial thickness. In this way, the surgeon can quickly modify the plane of dissection. Our results warrant the further investigation of applying iFC in larger, multicenter studies. |
| first_indexed | 2024-03-10T00:40:58Z |
| format | Article |
| id | doaj.art-2e28ecc78cab41319e706b75d695ec45 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T00:40:58Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-2e28ecc78cab41319e706b75d695ec452023-11-23T15:07:52ZengMDPI AGBiology2079-77372022-09-01119133910.3390/biology11091339Intraoperative Flow Cytometry for the Characterization of Gynecological MalignanciesZoi Anastasiadi0Stefania Mantziou1Christos Akrivis2Minas Paschopoulos3Eufemia Balasi4Georgios D. Lianos5George A. Alexiou6Michail Mitsis7George Vartholomatos8Georgios S. Markopoulos9Department of Obstetrics and Gynecology, ‘G. Chatzikosta’ General Hospital, 45001 Ioannina, GreeceHaematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, 45500 Ioannina, GreeceDepartment of Obstetrics and Gynecology, ‘G. Chatzikosta’ General Hospital, 45001 Ioannina, GreeceDepartment of Obstetrics and Gynecology, University Hospital of Ioannina, 45500 Ioannina, GreecePathology Department, ‘G. Chatzikosta’ General Hospital, 45001 Ioannina, GreeceDepartment of Surgery, University Hospital of Ioannina, 45500 Ioannina, GreeceDepartment of Neurosurgery, University Hospital of Ioannina, 45500 Ioannina, GreeceDepartment of Surgery, University Hospital of Ioannina, 45500 Ioannina, GreeceHaematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, 45500 Ioannina, GreeceHaematology Laboratory-Unit of Molecular Biology, University Hospital of Ioannina, 45500 Ioannina, GreeceCell-cycle analysis has shown the presence of aneuploidy to be associated with poor prognosis. We developed an innovative rapid cell-cycle analysis protocol (the Ioannina protocol) that permitted the intraoperative identification of neoplastic cells in a plethora of malignancies. Herein, we aimed to investigate the potential role of cell-cycle analysis in the intraoperative characterization of gynecological malignancies. Women who underwent surgery for gynecological malignancies in our institution over a three-year period were included in this study. Permanent section pathology evaluation was used as the gold standard for malignancy evaluation. Total accordance was observed between flow cytometry and pathology evaluation. In total, 21 aneuploid cancers were detected following DNA index calculation. Of these, 20 were hyperploid and 1 was hypoploid. In addition, tumor samples were characterized by a significantly lower percentage of cells in G0/G1, as well as an induced tumor index. The response time for flow cytometry to obtain results was 5–6 min per sample. It seems that flow cytometry analyses for intraoperative tumor evaluation can be safely expanded to gynecological malignancies. This is a novel practical approach that has been proven valuable in several tumor types to date, and also seems to be reliable for gynecological malignancies. Intraoperative flow cytometry is expected to be crucial in decisions of lymph node dissection in endometrial cancers, due to its rapid response regarding the tumor invasion of part or all of the myometrial thickness. In this way, the surgeon can quickly modify the plane of dissection. Our results warrant the further investigation of applying iFC in larger, multicenter studies.https://www.mdpi.com/2079-7737/11/9/1339cancersurgical treatmentflow cytometrygynecological malignanciessurgical oncology |
| spellingShingle | Zoi Anastasiadi Stefania Mantziou Christos Akrivis Minas Paschopoulos Eufemia Balasi Georgios D. Lianos George A. Alexiou Michail Mitsis George Vartholomatos Georgios S. Markopoulos Intraoperative Flow Cytometry for the Characterization of Gynecological Malignancies Biology cancer surgical treatment flow cytometry gynecological malignancies surgical oncology |
| title | Intraoperative Flow Cytometry for the Characterization of Gynecological Malignancies |
| title_full | Intraoperative Flow Cytometry for the Characterization of Gynecological Malignancies |
| title_fullStr | Intraoperative Flow Cytometry for the Characterization of Gynecological Malignancies |
| title_full_unstemmed | Intraoperative Flow Cytometry for the Characterization of Gynecological Malignancies |
| title_short | Intraoperative Flow Cytometry for the Characterization of Gynecological Malignancies |
| title_sort | intraoperative flow cytometry for the characterization of gynecological malignancies |
| topic | cancer surgical treatment flow cytometry gynecological malignancies surgical oncology |
| url | https://www.mdpi.com/2079-7737/11/9/1339 |
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