Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis
Propolis is a natural product proved to be efficient against Mycobacterium tuberculosis. Although it is produced by bees, its active alcoholic-aqueous fraction contains plant-derived molecules. To gain some insight into its mechanism of antimycobacterial activity, we studied the metabolic changes in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.929476/full |
| _version_ | 1811333563526676480 |
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| author | Rafał Sawicki Jarosław Widelski Piotr Okińczyc Wiesław Truszkiewicz Joanna Glous Elwira Sieniawska |
| author_facet | Rafał Sawicki Jarosław Widelski Piotr Okińczyc Wiesław Truszkiewicz Joanna Glous Elwira Sieniawska |
| author_sort | Rafał Sawicki |
| collection | DOAJ |
| description | Propolis is a natural product proved to be efficient against Mycobacterium tuberculosis. Although it is produced by bees, its active alcoholic-aqueous fraction contains plant-derived molecules. To gain some insight into its mechanism of antimycobacterial activity, we studied the metabolic changes in bacterial cells treated with extract of Trigona sp. propolis from Nepal. The detailed metabolomic and transcriptomic analysis performed in this study indicated target points in bacterial cells under propolis extract influence. The profile of lipids forming the outer and middle layer of the mycobacterial cell envelope was not changed by propolis treatment, however, fluctuations in the profiles of amphipathic glycerophospholipids were observed. The enrichment analysis revealed bacterial metabolic pathways affected by Trigona sp. propolis treatment. The early metabolic response involved much more pathways than observed after 48 h of incubation, however, the highest enrichment ratio was observed after 48 h, indicating the long-lasting influence of propolis. The early bacterial response was related to the increased demand for energy and upregulation of molecules involved in the formation of the cell membrane. The transcriptomic analysis confirmed that bacteria also suffered from oxidative stress, which was more pronounced on the second day of exposure. This was the first attempt to explain the action of Nepalese propolis extract against mycobacteria. |
| first_indexed | 2024-04-13T16:54:49Z |
| format | Article |
| id | doaj.art-2e2dada1265f4ec7ad52970209c7ecf5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T16:54:49Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2e2dada1265f4ec7ad52970209c7ecf52022-12-22T02:38:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.929476929476Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosisRafał Sawicki0Jarosław Widelski1Piotr Okińczyc2Wiesław Truszkiewicz3Joanna Glous4Elwira Sieniawska5Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, PolandDepartment of Pharmacognosy, Medical University of Lublin, Lublin, PolandDepartment of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Wrocław, PolandChair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, PolandChair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, PolandDepartment of Natural Products Chemistry, Medical University of Lublin, Lublin, PolandPropolis is a natural product proved to be efficient against Mycobacterium tuberculosis. Although it is produced by bees, its active alcoholic-aqueous fraction contains plant-derived molecules. To gain some insight into its mechanism of antimycobacterial activity, we studied the metabolic changes in bacterial cells treated with extract of Trigona sp. propolis from Nepal. The detailed metabolomic and transcriptomic analysis performed in this study indicated target points in bacterial cells under propolis extract influence. The profile of lipids forming the outer and middle layer of the mycobacterial cell envelope was not changed by propolis treatment, however, fluctuations in the profiles of amphipathic glycerophospholipids were observed. The enrichment analysis revealed bacterial metabolic pathways affected by Trigona sp. propolis treatment. The early metabolic response involved much more pathways than observed after 48 h of incubation, however, the highest enrichment ratio was observed after 48 h, indicating the long-lasting influence of propolis. The early bacterial response was related to the increased demand for energy and upregulation of molecules involved in the formation of the cell membrane. The transcriptomic analysis confirmed that bacteria also suffered from oxidative stress, which was more pronounced on the second day of exposure. This was the first attempt to explain the action of Nepalese propolis extract against mycobacteria.https://www.frontiersin.org/articles/10.3389/fmicb.2022.929476/fullflavonoidsmetabolomicsLC-MSsigma factorsoxidative stressnatural products |
| spellingShingle | Rafał Sawicki Jarosław Widelski Piotr Okińczyc Wiesław Truszkiewicz Joanna Glous Elwira Sieniawska Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis Frontiers in Microbiology flavonoids metabolomics LC-MS sigma factors oxidative stress natural products |
| title | Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis |
| title_full | Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis |
| title_fullStr | Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis |
| title_full_unstemmed | Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis |
| title_short | Exposure to Nepalese Propolis Alters the Metabolic State of Mycobacterium tuberculosis |
| title_sort | exposure to nepalese propolis alters the metabolic state of mycobacterium tuberculosis |
| topic | flavonoids metabolomics LC-MS sigma factors oxidative stress natural products |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.929476/full |
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