IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact
Periodontal ligament (PDL) cells help maintain tissue homeostasis by balancing PDL tissue inflammation and regeneration. However, the mechanisms by which interferon γ (IFNγ) modulate this process are not yet fully understood. The present study aimed to examine the effect of primed and non-primed PDL...
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| Format: | Article |
| Language: | English |
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The Royal Society
2022-07-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.220056 |
| _version_ | 1797840259323527168 |
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| author | Weerachai Singhatanadgit Setthawut Kitpakornsanti Montree Toso Prasit Pavasant |
| author_facet | Weerachai Singhatanadgit Setthawut Kitpakornsanti Montree Toso Prasit Pavasant |
| author_sort | Weerachai Singhatanadgit |
| collection | DOAJ |
| description | Periodontal ligament (PDL) cells help maintain tissue homeostasis by balancing PDL tissue inflammation and regeneration. However, the mechanisms by which interferon γ (IFNγ) modulate this process are not yet fully understood. The present study aimed to examine the effect of primed and non-primed PDL cells with IFNγ on the viability and differentiation of T lymphocytes and its functional consequences. The results showed that IFNγ-primed PDL cells possessed enhanced immunosuppression by suppressing T-lymphocyte viability and directing T-lymphocyte differentiation towards a higher T helper (Th) Th2/Th1 ratio. Suppression of T-cell viability was mainly mediated by IFNγ-inducible secreted mediators, which was prevented in the presence of direct cell contact, probably by intercellular adhesion molecule-1 (ICAM-1)-induced PI3 K-mediated transforming growth factor β1 expression in PDL cells. By contrast, ICAM-1 activation augmented IFNγ-induced IFNγ and interleukin-6 expression in PDL cells, which in turn modulated T-cell differentiation. The resulting interaction between these two cell types activated macrophage and suppressed osteoclast differentiation. In conclusion, the results have shown, for the first time to our knowledge, that primed and non-primed PDL cells with IFNγ differentially control T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact, suggesting the role of PDL cells in shifting an inflammatory phase towards a regenerative phase. |
| first_indexed | 2024-04-09T16:12:01Z |
| format | Article |
| id | doaj.art-2e2f710812ae4383b250279ddd349b96 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-04-09T16:12:01Z |
| publishDate | 2022-07-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-2e2f710812ae4383b250279ddd349b962023-04-24T09:20:17ZengThe Royal SocietyRoyal Society Open Science2054-57032022-07-019710.1098/rsos.220056IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contactWeerachai Singhatanadgit0Setthawut Kitpakornsanti1Montree Toso2Prasit Pavasant3Oral and Maxillofacial Surgery Unit, Faculty of Dentistry, Thammasat University, Rangsit Campus, Pathumthani, ThailandResearch Unit in Mineralized Tissue Reconstruction, Thammasat University, Rangsit Campus, Pathumthani, ThailandResearch Unit in Mineralized Tissue Reconstruction, Thammasat University, Rangsit Campus, Pathumthani, ThailandCenter of Excellence in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, ThailandPeriodontal ligament (PDL) cells help maintain tissue homeostasis by balancing PDL tissue inflammation and regeneration. However, the mechanisms by which interferon γ (IFNγ) modulate this process are not yet fully understood. The present study aimed to examine the effect of primed and non-primed PDL cells with IFNγ on the viability and differentiation of T lymphocytes and its functional consequences. The results showed that IFNγ-primed PDL cells possessed enhanced immunosuppression by suppressing T-lymphocyte viability and directing T-lymphocyte differentiation towards a higher T helper (Th) Th2/Th1 ratio. Suppression of T-cell viability was mainly mediated by IFNγ-inducible secreted mediators, which was prevented in the presence of direct cell contact, probably by intercellular adhesion molecule-1 (ICAM-1)-induced PI3 K-mediated transforming growth factor β1 expression in PDL cells. By contrast, ICAM-1 activation augmented IFNγ-induced IFNγ and interleukin-6 expression in PDL cells, which in turn modulated T-cell differentiation. The resulting interaction between these two cell types activated macrophage and suppressed osteoclast differentiation. In conclusion, the results have shown, for the first time to our knowledge, that primed and non-primed PDL cells with IFNγ differentially control T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact, suggesting the role of PDL cells in shifting an inflammatory phase towards a regenerative phase.https://royalsocietypublishing.org/doi/10.1098/rsos.220056interferon γperiodontal ligament cellsT lymphocytesintercellular adhesion molecule-1transforming growth factor β1immunosuppression |
| spellingShingle | Weerachai Singhatanadgit Setthawut Kitpakornsanti Montree Toso Prasit Pavasant IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact Royal Society Open Science interferon γ periodontal ligament cells T lymphocytes intercellular adhesion molecule-1 transforming growth factor β1 immunosuppression |
| title | IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact |
| title_full | IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact |
| title_fullStr | IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact |
| title_full_unstemmed | IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact |
| title_short | IFNγ-primed periodontal ligament cells regulate T-cell responses via IFNγ-inducible mediators and ICAM-1-mediated direct cell contact |
| title_sort | ifnγ primed periodontal ligament cells regulate t cell responses via ifnγ inducible mediators and icam 1 mediated direct cell contact |
| topic | interferon γ periodontal ligament cells T lymphocytes intercellular adhesion molecule-1 transforming growth factor β1 immunosuppression |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.220056 |
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